Genome In Silico and In Vitro Analysis of the Probiotic Properties of a Bacterial Endophyte, Bacillus Paranthracis Strain MHSD3

Spore-forming Bacillus species are gaining interest in human health recently, due to their ability to withstand the harsh environment of the gastrointestinal tract. The present study explores probiotic features of Bacillus paranthracis strain MHSD3 through genomic analysis and in vitro probiotic ass...

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Autores principales: Mamonokane Olga Diale, Eugenie Kayitesi, Mahloro Hope Serepa-Dlamini
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Publicado: Frontiers Media S.A. 2021
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Acceso en línea:https://doaj.org/article/628a85a044d740d28093fde01df38d7c
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spelling oai:doaj.org-article:628a85a044d740d28093fde01df38d7c2021-11-11T05:33:18ZGenome In Silico and In Vitro Analysis of the Probiotic Properties of a Bacterial Endophyte, Bacillus Paranthracis Strain MHSD31664-802110.3389/fgene.2021.672149https://doaj.org/article/628a85a044d740d28093fde01df38d7c2021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fgene.2021.672149/fullhttps://doaj.org/toc/1664-8021Spore-forming Bacillus species are gaining interest in human health recently, due to their ability to withstand the harsh environment of the gastrointestinal tract. The present study explores probiotic features of Bacillus paranthracis strain MHSD3 through genomic analysis and in vitro probiotic assays. The draft genome of strain MHSD3 contained genes associated with tolerance to gastrointestinal stress and adhesion. Cluster genes responsible for the synthesis of antimicrobial non-ribosomal peptide synthetases, bacteriocins, and linear azole-containing peptides were identified. Additionally, strain MHSD3 was able to survive in an acidic environment, had the tolerance to bile salt, and exhibited the capability to tolerate gastric juices. Moreover, the isolate was found to possess strong cell surface traits such as high auto-aggregation and hydrophobicity indices of 79 and 54%, respectively. Gas chromatography–mass spectrometry analysis showed that the strain produced secondary metabolites such as amino acids, phenolic compounds, and organic acid, known to exert health-promoting properties, including the improvement of gastrointestinal tract health.Mamonokane Olga DialeEugenie KayitesiMahloro Hope Serepa-DlaminiFrontiers Media S.A.articleBacillus paranthracisprobioticsbiosynthetic gene clustersendophytessecondary metabolitesGeneticsQH426-470ENFrontiers in Genetics, Vol 12 (2021)
institution DOAJ
collection DOAJ
language EN
topic Bacillus paranthracis
probiotics
biosynthetic gene clusters
endophytes
secondary metabolites
Genetics
QH426-470
spellingShingle Bacillus paranthracis
probiotics
biosynthetic gene clusters
endophytes
secondary metabolites
Genetics
QH426-470
Mamonokane Olga Diale
Eugenie Kayitesi
Mahloro Hope Serepa-Dlamini
Genome In Silico and In Vitro Analysis of the Probiotic Properties of a Bacterial Endophyte, Bacillus Paranthracis Strain MHSD3
description Spore-forming Bacillus species are gaining interest in human health recently, due to their ability to withstand the harsh environment of the gastrointestinal tract. The present study explores probiotic features of Bacillus paranthracis strain MHSD3 through genomic analysis and in vitro probiotic assays. The draft genome of strain MHSD3 contained genes associated with tolerance to gastrointestinal stress and adhesion. Cluster genes responsible for the synthesis of antimicrobial non-ribosomal peptide synthetases, bacteriocins, and linear azole-containing peptides were identified. Additionally, strain MHSD3 was able to survive in an acidic environment, had the tolerance to bile salt, and exhibited the capability to tolerate gastric juices. Moreover, the isolate was found to possess strong cell surface traits such as high auto-aggregation and hydrophobicity indices of 79 and 54%, respectively. Gas chromatography–mass spectrometry analysis showed that the strain produced secondary metabolites such as amino acids, phenolic compounds, and organic acid, known to exert health-promoting properties, including the improvement of gastrointestinal tract health.
format article
author Mamonokane Olga Diale
Eugenie Kayitesi
Mahloro Hope Serepa-Dlamini
author_facet Mamonokane Olga Diale
Eugenie Kayitesi
Mahloro Hope Serepa-Dlamini
author_sort Mamonokane Olga Diale
title Genome In Silico and In Vitro Analysis of the Probiotic Properties of a Bacterial Endophyte, Bacillus Paranthracis Strain MHSD3
title_short Genome In Silico and In Vitro Analysis of the Probiotic Properties of a Bacterial Endophyte, Bacillus Paranthracis Strain MHSD3
title_full Genome In Silico and In Vitro Analysis of the Probiotic Properties of a Bacterial Endophyte, Bacillus Paranthracis Strain MHSD3
title_fullStr Genome In Silico and In Vitro Analysis of the Probiotic Properties of a Bacterial Endophyte, Bacillus Paranthracis Strain MHSD3
title_full_unstemmed Genome In Silico and In Vitro Analysis of the Probiotic Properties of a Bacterial Endophyte, Bacillus Paranthracis Strain MHSD3
title_sort genome in silico and in vitro analysis of the probiotic properties of a bacterial endophyte, bacillus paranthracis strain mhsd3
publisher Frontiers Media S.A.
publishDate 2021
url https://doaj.org/article/628a85a044d740d28093fde01df38d7c
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AT eugeniekayitesi genomeinsilicoandinvitroanalysisoftheprobioticpropertiesofabacterialendophytebacillusparanthracisstrainmhsd3
AT mahlorohopeserepadlamini genomeinsilicoandinvitroanalysisoftheprobioticpropertiesofabacterialendophytebacillusparanthracisstrainmhsd3
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