Genome In Silico and In Vitro Analysis of the Probiotic Properties of a Bacterial Endophyte, Bacillus Paranthracis Strain MHSD3

Spore-forming Bacillus species are gaining interest in human health recently, due to their ability to withstand the harsh environment of the gastrointestinal tract. The present study explores probiotic features of Bacillus paranthracis strain MHSD3 through genomic analysis and in vitro probiotic ass...

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Autores principales:	Mamonokane Olga Diale, Eugenie Kayitesi, Mahloro Hope Serepa-Dlamini
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	Bacillus paranthracis probiotics biosynthetic gene clusters endophytes secondary metabolites Genetics QH426-470
Acceso en línea:	https://doaj.org/article/628a85a044d740d28093fde01df38d7c
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spelling	oai:doaj.org-article:628a85a044d740d28093fde01df38d7c2021-11-11T05:33:18ZGenome In Silico and In Vitro Analysis of the Probiotic Properties of a Bacterial Endophyte, Bacillus Paranthracis Strain MHSD31664-802110.3389/fgene.2021.672149https://doaj.org/article/628a85a044d740d28093fde01df38d7c2021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fgene.2021.672149/fullhttps://doaj.org/toc/1664-8021Spore-forming Bacillus species are gaining interest in human health recently, due to their ability to withstand the harsh environment of the gastrointestinal tract. The present study explores probiotic features of Bacillus paranthracis strain MHSD3 through genomic analysis and in vitro probiotic assays. The draft genome of strain MHSD3 contained genes associated with tolerance to gastrointestinal stress and adhesion. Cluster genes responsible for the synthesis of antimicrobial non-ribosomal peptide synthetases, bacteriocins, and linear azole-containing peptides were identified. Additionally, strain MHSD3 was able to survive in an acidic environment, had the tolerance to bile salt, and exhibited the capability to tolerate gastric juices. Moreover, the isolate was found to possess strong cell surface traits such as high auto-aggregation and hydrophobicity indices of 79 and 54%, respectively. Gas chromatography–mass spectrometry analysis showed that the strain produced secondary metabolites such as amino acids, phenolic compounds, and organic acid, known to exert health-promoting properties, including the improvement of gastrointestinal tract health.Mamonokane Olga DialeEugenie KayitesiMahloro Hope Serepa-DlaminiFrontiers Media S.A.articleBacillus paranthracisprobioticsbiosynthetic gene clustersendophytessecondary metabolitesGeneticsQH426-470ENFrontiers in Genetics, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Bacillus paranthracis probiotics biosynthetic gene clusters endophytes secondary metabolites Genetics QH426-470
spellingShingle	Bacillus paranthracis probiotics biosynthetic gene clusters endophytes secondary metabolites Genetics QH426-470 Mamonokane Olga Diale Eugenie Kayitesi Mahloro Hope Serepa-Dlamini Genome In Silico and In Vitro Analysis of the Probiotic Properties of a Bacterial Endophyte, Bacillus Paranthracis Strain MHSD3
description	Spore-forming Bacillus species are gaining interest in human health recently, due to their ability to withstand the harsh environment of the gastrointestinal tract. The present study explores probiotic features of Bacillus paranthracis strain MHSD3 through genomic analysis and in vitro probiotic assays. The draft genome of strain MHSD3 contained genes associated with tolerance to gastrointestinal stress and adhesion. Cluster genes responsible for the synthesis of antimicrobial non-ribosomal peptide synthetases, bacteriocins, and linear azole-containing peptides were identified. Additionally, strain MHSD3 was able to survive in an acidic environment, had the tolerance to bile salt, and exhibited the capability to tolerate gastric juices. Moreover, the isolate was found to possess strong cell surface traits such as high auto-aggregation and hydrophobicity indices of 79 and 54%, respectively. Gas chromatography–mass spectrometry analysis showed that the strain produced secondary metabolites such as amino acids, phenolic compounds, and organic acid, known to exert health-promoting properties, including the improvement of gastrointestinal tract health.
format	article
author	Mamonokane Olga Diale Eugenie Kayitesi Mahloro Hope Serepa-Dlamini
author_facet	Mamonokane Olga Diale Eugenie Kayitesi Mahloro Hope Serepa-Dlamini
author_sort	Mamonokane Olga Diale
title	Genome In Silico and In Vitro Analysis of the Probiotic Properties of a Bacterial Endophyte, Bacillus Paranthracis Strain MHSD3
title_short	Genome In Silico and In Vitro Analysis of the Probiotic Properties of a Bacterial Endophyte, Bacillus Paranthracis Strain MHSD3
title_full	Genome In Silico and In Vitro Analysis of the Probiotic Properties of a Bacterial Endophyte, Bacillus Paranthracis Strain MHSD3
title_fullStr	Genome In Silico and In Vitro Analysis of the Probiotic Properties of a Bacterial Endophyte, Bacillus Paranthracis Strain MHSD3
title_full_unstemmed	Genome In Silico and In Vitro Analysis of the Probiotic Properties of a Bacterial Endophyte, Bacillus Paranthracis Strain MHSD3
title_sort	genome in silico and in vitro analysis of the probiotic properties of a bacterial endophyte, bacillus paranthracis strain mhsd3
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/628a85a044d740d28093fde01df38d7c
work_keys_str_mv	AT mamonokaneolgadiale genomeinsilicoandinvitroanalysisoftheprobioticpropertiesofabacterialendophytebacillusparanthracisstrainmhsd3 AT eugeniekayitesi genomeinsilicoandinvitroanalysisoftheprobioticpropertiesofabacterialendophytebacillusparanthracisstrainmhsd3 AT mahlorohopeserepadlamini genomeinsilicoandinvitroanalysisoftheprobioticpropertiesofabacterialendophytebacillusparanthracisstrainmhsd3
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Genome In Silico and In Vitro Analysis of the Probiotic Properties of a Bacterial Endophyte, Bacillus Paranthracis Strain MHSD3

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