Photothermal effects of NaYF4:Yb,Er@PE3@Fe3O4 superparamagnetic nanoprobes in the treatment of melanoma

Xue Wang,1 Chunyang Kang,2 Ying Pan,1 Rihua Jiang31Department of Obstetrics & Gynecology, China–Japan Union Hospital of Jilin University, Changchun, People’s Republic of China; 2Department of Neurology, China–Japan Union Hospital of Jilin University, Changchun,...

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Autores principales:	Wang X, Kang C, Pan Y, Jiang R
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2019
Materias:	upconversion nanoparticles near infrared photothermal therapy melanoma heat shock protein 70 (HSP70) Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/628fbc29e1ce4b379d9742a0bcd0c545
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spelling	oai:doaj.org-article:628fbc29e1ce4b379d9742a0bcd0c5452021-12-02T05:43:36ZPhotothermal effects of NaYF4:Yb,Er@PE3@Fe3O4 superparamagnetic nanoprobes in the treatment of melanoma1178-2013https://doaj.org/article/628fbc29e1ce4b379d9742a0bcd0c5452019-06-01T00:00:00Zhttps://www.dovepress.com/photothermal-effects-of-nayf4yberpe3fe3o4-superparamagnetic-nanoprobes-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Xue Wang,1 Chunyang Kang,2 Ying Pan,1 Rihua Jiang31Department of Obstetrics & Gynecology, China–Japan Union Hospital of Jilin University, Changchun, People’s Republic of China; 2Department of Neurology, China–Japan Union Hospital of Jilin University, Changchun, People’s Republic of China; 3Department of Dermatology, China–Japan Union Hospital of Jilin University, Changchun, People’s Republic of ChinaObjective: The study aimed to synthesize superparamagnetic NaYF4:Yb,Er@PE3@Fe3O4 upconversion nanoprobes and to study their photothermal effects for the treatment of malignant melanoma.Methods: Morphological characteristics of the synthesized nanoprobes were examined by scanning electron microscopy. Their biocompatibility and biodistribution profiles were assessed through blood routine/biochemistry tests and the inductively coupled plasma/optical emission spectrometry-based analysis of tissue metal elements. Their photothermal conversion efficiency and their potential as contrast agents for upconversion luminescence (UCL)/magnetic resonance imaging (MRI) dual-modal imaging were tested. Efficacy in photothermal therapy, which was achieved by combining nanoprobes with near-infrared (NIR) irradiation, was evaluated in both A375 cell line and BALB/c mice models. The underlying mechanisms were interrogated by molecular approaches including the MTT assay, flow cytometry, semiquantitative PCR, western blot, and immunohistochemistry.Results: 1) Our synthesized NaYF4:Yb,Er@PE3@Fe3O4 nanoprobes exhibited a uniform cubic morphology with a diameter of &sim;50 nm. Subcutaneous administration led to no severe, long-lasting adverse effects in mice, possibly due to complete removal of these nanomaterials within one month. 2) Our nanoprobes possessed superior photothermal conversion efficiency and strong contrasting effects during UCL/MRI dual-modal imaging, corroborating their applications in imaging-guided photothermal therapy. 3) Combinatorial treatment of these nanoprobes with NIR irradiation induced profound apoptosis/necrosis in A375 cells. Similarly, the same treatment modality led to strong therapeutic effects in BALB/c mice implanted with A375 tumor xenografts. Mechanistic studies suggested an involvement of heat shock protein 70 in mediating the observed antitumor effects of our nanoprobes.Conclusion: Our study describes a convenient method to synthesize a new type of superparamagnetic upconversion nanoprobes, which possess high biocompatibility and can be used in imaging-guided photothermal therapy for the treatment of malignant melanoma. Importantly, our findings will promote clinical applications of NaYF4:Yb,Er@PE3@Fe3O4 as novel theranostic agents in treating melanoma and many other tumors.Keywords: upconversion nanoparticles, near infrared, photothermal therapy, melanoma, heat shock protein 70Wang XKang CPan YJiang RDove Medical Pressarticleupconversion nanoparticlesnear infraredphotothermal therapymelanomaheat shock protein 70 (HSP70)Medicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 14, Pp 4319-4331 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	upconversion nanoparticles near infrared photothermal therapy melanoma heat shock protein 70 (HSP70) Medicine (General) R5-920
spellingShingle	upconversion nanoparticles near infrared photothermal therapy melanoma heat shock protein 70 (HSP70) Medicine (General) R5-920 Wang X Kang C Pan Y Jiang R Photothermal effects of NaYF4:Yb,Er@PE3@Fe3O4 superparamagnetic nanoprobes in the treatment of melanoma
description	Xue Wang,1 Chunyang Kang,2 Ying Pan,1 Rihua Jiang31Department of Obstetrics & Gynecology, China–Japan Union Hospital of Jilin University, Changchun, People’s Republic of China; 2Department of Neurology, China–Japan Union Hospital of Jilin University, Changchun, People’s Republic of China; 3Department of Dermatology, China–Japan Union Hospital of Jilin University, Changchun, People’s Republic of ChinaObjective: The study aimed to synthesize superparamagnetic NaYF4:Yb,Er@PE3@Fe3O4 upconversion nanoprobes and to study their photothermal effects for the treatment of malignant melanoma.Methods: Morphological characteristics of the synthesized nanoprobes were examined by scanning electron microscopy. Their biocompatibility and biodistribution profiles were assessed through blood routine/biochemistry tests and the inductively coupled plasma/optical emission spectrometry-based analysis of tissue metal elements. Their photothermal conversion efficiency and their potential as contrast agents for upconversion luminescence (UCL)/magnetic resonance imaging (MRI) dual-modal imaging were tested. Efficacy in photothermal therapy, which was achieved by combining nanoprobes with near-infrared (NIR) irradiation, was evaluated in both A375 cell line and BALB/c mice models. The underlying mechanisms were interrogated by molecular approaches including the MTT assay, flow cytometry, semiquantitative PCR, western blot, and immunohistochemistry.Results: 1) Our synthesized NaYF4:Yb,Er@PE3@Fe3O4 nanoprobes exhibited a uniform cubic morphology with a diameter of &sim;50 nm. Subcutaneous administration led to no severe, long-lasting adverse effects in mice, possibly due to complete removal of these nanomaterials within one month. 2) Our nanoprobes possessed superior photothermal conversion efficiency and strong contrasting effects during UCL/MRI dual-modal imaging, corroborating their applications in imaging-guided photothermal therapy. 3) Combinatorial treatment of these nanoprobes with NIR irradiation induced profound apoptosis/necrosis in A375 cells. Similarly, the same treatment modality led to strong therapeutic effects in BALB/c mice implanted with A375 tumor xenografts. Mechanistic studies suggested an involvement of heat shock protein 70 in mediating the observed antitumor effects of our nanoprobes.Conclusion: Our study describes a convenient method to synthesize a new type of superparamagnetic upconversion nanoprobes, which possess high biocompatibility and can be used in imaging-guided photothermal therapy for the treatment of malignant melanoma. Importantly, our findings will promote clinical applications of NaYF4:Yb,Er@PE3@Fe3O4 as novel theranostic agents in treating melanoma and many other tumors.Keywords: upconversion nanoparticles, near infrared, photothermal therapy, melanoma, heat shock protein 70
format	article
author	Wang X Kang C Pan Y Jiang R
author_facet	Wang X Kang C Pan Y Jiang R
author_sort	Wang X
title	Photothermal effects of NaYF4:Yb,Er@PE3@Fe3O4 superparamagnetic nanoprobes in the treatment of melanoma
title_short	Photothermal effects of NaYF4:Yb,Er@PE3@Fe3O4 superparamagnetic nanoprobes in the treatment of melanoma
title_full	Photothermal effects of NaYF4:Yb,Er@PE3@Fe3O4 superparamagnetic nanoprobes in the treatment of melanoma
title_fullStr	Photothermal effects of NaYF4:Yb,Er@PE3@Fe3O4 superparamagnetic nanoprobes in the treatment of melanoma
title_full_unstemmed	Photothermal effects of NaYF4:Yb,Er@PE3@Fe3O4 superparamagnetic nanoprobes in the treatment of melanoma
title_sort	photothermal effects of nayf4:yb,er@pe3@fe3o4 superparamagnetic nanoprobes in the treatment of melanoma
publisher	Dove Medical Press
publishDate	2019
url	https://doaj.org/article/628fbc29e1ce4b379d9742a0bcd0c545
work_keys_str_mv	AT wangx photothermaleffectsofnayf4yberpe3fe3o4superparamagneticnanoprobesinthetreatmentofmelanoma AT kangc photothermaleffectsofnayf4yberpe3fe3o4superparamagneticnanoprobesinthetreatmentofmelanoma AT pany photothermaleffectsofnayf4yberpe3fe3o4superparamagneticnanoprobesinthetreatmentofmelanoma AT jiangr photothermaleffectsofnayf4yberpe3fe3o4superparamagneticnanoprobesinthetreatmentofmelanoma
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Photothermal effects of NaYF4:Yb,Er@PE3@Fe3O4 superparamagnetic nanoprobes in the treatment of melanoma

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