Protocol for assessing the role of hippocampal perineuronal nets in aversive memories

Summary: Perineuronal nets (PNNs) are emerging as critical regulators of memory-related neuronal processes. However, their exact contribution depends on type of memory, consolidation stage, or brain region, and remains to be fully investigated. We describe here a protocol to evaluate the importance...

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Autores principales:	Vladimir Jovasevic, Hui Zhang, Zorica Petrovic, Ana Cicvaric, Jelena Radulovic
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Behavior Microscopy Model Organisms Neuroscience Science (General) Q1-390
Acceso en línea:	https://doaj.org/article/62952a2c7669439e8c0ac38687b09e24
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spelling	oai:doaj.org-article:62952a2c7669439e8c0ac38687b09e242021-11-04T04:39:52ZProtocol for assessing the role of hippocampal perineuronal nets in aversive memories2666-166710.1016/j.xpro.2021.100931https://doaj.org/article/62952a2c7669439e8c0ac38687b09e242021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2666166721006377https://doaj.org/toc/2666-1667Summary: Perineuronal nets (PNNs) are emerging as critical regulators of memory-related neuronal processes. However, their exact contribution depends on type of memory, consolidation stage, or brain region, and remains to be fully investigated. We describe here a protocol to evaluate the importance of PNNs in the dorsal hippocampus in different stages of aversive memories using a mouse model. The protocol provides detailed instructions for surgical implantation of hippocampal cannulas, drug infusion, contextual fear conditioning procedures, and immunohistochemistry for PNN visualization.For complete details on the use and execution of this protocol, please refer to Jovasevic et al. (2021).Vladimir JovasevicHui ZhangZorica PetrovicAna CicvaricJelena RadulovicElsevierarticleBehaviorMicroscopyModel OrganismsNeuroscienceScience (General)Q1-390ENSTAR Protocols, Vol 2, Iss 4, Pp 100931- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Behavior Microscopy Model Organisms Neuroscience Science (General) Q1-390
spellingShingle	Behavior Microscopy Model Organisms Neuroscience Science (General) Q1-390 Vladimir Jovasevic Hui Zhang Zorica Petrovic Ana Cicvaric Jelena Radulovic Protocol for assessing the role of hippocampal perineuronal nets in aversive memories
description	Summary: Perineuronal nets (PNNs) are emerging as critical regulators of memory-related neuronal processes. However, their exact contribution depends on type of memory, consolidation stage, or brain region, and remains to be fully investigated. We describe here a protocol to evaluate the importance of PNNs in the dorsal hippocampus in different stages of aversive memories using a mouse model. The protocol provides detailed instructions for surgical implantation of hippocampal cannulas, drug infusion, contextual fear conditioning procedures, and immunohistochemistry for PNN visualization.For complete details on the use and execution of this protocol, please refer to Jovasevic et al. (2021).
format	article
author	Vladimir Jovasevic Hui Zhang Zorica Petrovic Ana Cicvaric Jelena Radulovic
author_facet	Vladimir Jovasevic Hui Zhang Zorica Petrovic Ana Cicvaric Jelena Radulovic
author_sort	Vladimir Jovasevic
title	Protocol for assessing the role of hippocampal perineuronal nets in aversive memories
title_short	Protocol for assessing the role of hippocampal perineuronal nets in aversive memories
title_full	Protocol for assessing the role of hippocampal perineuronal nets in aversive memories
title_fullStr	Protocol for assessing the role of hippocampal perineuronal nets in aversive memories
title_full_unstemmed	Protocol for assessing the role of hippocampal perineuronal nets in aversive memories
title_sort	protocol for assessing the role of hippocampal perineuronal nets in aversive memories
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/62952a2c7669439e8c0ac38687b09e24
work_keys_str_mv	AT vladimirjovasevic protocolforassessingtheroleofhippocampalperineuronalnetsinaversivememories AT huizhang protocolforassessingtheroleofhippocampalperineuronalnetsinaversivememories AT zoricapetrovic protocolforassessingtheroleofhippocampalperineuronalnetsinaversivememories AT anacicvaric protocolforassessingtheroleofhippocampalperineuronalnetsinaversivememories AT jelenaradulovic protocolforassessingtheroleofhippocampalperineuronalnetsinaversivememories
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Protocol for assessing the role of hippocampal perineuronal nets in aversive memories

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