Mass Spectrometry Imaging (MSI) Delineates Thymus-Centric Metabolism In Vivo as an Effect of Systemic Administration of Dexamethasone

Matrix-assisted laser desorption ionization mass spectrometry imaging (MALDI-MSI) is increasingly used in a broad range of research due to its ability to visualize the spatial distribution of metabolites in vivo. Here, we have developed a method, named thoracic Mass Spectrometry Imaging (tMSI), as a...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Yudai Tsuji, Shinichi Yamaguchi, Tomoyuki Nakamura, Masaya Ikegawa
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	MALDI-MSI thymus purine and pyrimidine dexamethasone UMAP apoptosis Technology T Engineering (General). Civil engineering (General) TA1-2040 Biology (General) QH301-705.5 Physics QC1-999 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/62968cb0909a47fc86fbb4c4b141c5c9
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:62968cb0909a47fc86fbb4c4b141c5c9
record_format	dspace
spelling	oai:doaj.org-article:62968cb0909a47fc86fbb4c4b141c5c92021-11-25T16:43:12ZMass Spectrometry Imaging (MSI) Delineates Thymus-Centric Metabolism In Vivo as an Effect of Systemic Administration of Dexamethasone10.3390/app1122110382076-3417https://doaj.org/article/62968cb0909a47fc86fbb4c4b141c5c92021-11-01T00:00:00Zhttps://www.mdpi.com/2076-3417/11/22/11038https://doaj.org/toc/2076-3417Matrix-assisted laser desorption ionization mass spectrometry imaging (MALDI-MSI) is increasingly used in a broad range of research due to its ability to visualize the spatial distribution of metabolites in vivo. Here, we have developed a method, named thoracic Mass Spectrometry Imaging (tMSI), as a standard protocol of molecular imaging of whole-animal sectioning in various settings of mice in vivo. Further application of the strategy that involved the systemic administration of dexamethasone (DEX) in mice, enabled a dynamic shift in the energy status of multiple thoracic organs to be visualized, based on tMSI data of purine and pyrimidine metabolites. Furthermore, with the introduction of uniform manifold approximation and projection (UMAP) for tMSI data, metabolic profiles normally localized in the cortex and cortico-medullary junction (CMJ) of the thymus were drastically shifted as minor profiles into the medulla of DEX-treated thymus. As a massive apoptotic cell death in the thymic cortex was noticeable, a single molecule, which was upregulated in the cortex of the thymus, enabled us to predict ongoing immunosuppression by in vivo DEX-administration.Yudai TsujiShinichi YamaguchiTomoyuki NakamuraMasaya IkegawaMDPI AGarticleMALDI-MSIthymuspurine and pyrimidinedexamethasoneUMAPapoptosisTechnologyTEngineering (General). Civil engineering (General)TA1-2040Biology (General)QH301-705.5PhysicsQC1-999ChemistryQD1-999ENApplied Sciences, Vol 11, Iss 11038, p 11038 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	MALDI-MSI thymus purine and pyrimidine dexamethasone UMAP apoptosis Technology T Engineering (General). Civil engineering (General) TA1-2040 Biology (General) QH301-705.5 Physics QC1-999 Chemistry QD1-999
spellingShingle	MALDI-MSI thymus purine and pyrimidine dexamethasone UMAP apoptosis Technology T Engineering (General). Civil engineering (General) TA1-2040 Biology (General) QH301-705.5 Physics QC1-999 Chemistry QD1-999 Yudai Tsuji Shinichi Yamaguchi Tomoyuki Nakamura Masaya Ikegawa Mass Spectrometry Imaging (MSI) Delineates Thymus-Centric Metabolism In Vivo as an Effect of Systemic Administration of Dexamethasone
description	Matrix-assisted laser desorption ionization mass spectrometry imaging (MALDI-MSI) is increasingly used in a broad range of research due to its ability to visualize the spatial distribution of metabolites in vivo. Here, we have developed a method, named thoracic Mass Spectrometry Imaging (tMSI), as a standard protocol of molecular imaging of whole-animal sectioning in various settings of mice in vivo. Further application of the strategy that involved the systemic administration of dexamethasone (DEX) in mice, enabled a dynamic shift in the energy status of multiple thoracic organs to be visualized, based on tMSI data of purine and pyrimidine metabolites. Furthermore, with the introduction of uniform manifold approximation and projection (UMAP) for tMSI data, metabolic profiles normally localized in the cortex and cortico-medullary junction (CMJ) of the thymus were drastically shifted as minor profiles into the medulla of DEX-treated thymus. As a massive apoptotic cell death in the thymic cortex was noticeable, a single molecule, which was upregulated in the cortex of the thymus, enabled us to predict ongoing immunosuppression by in vivo DEX-administration.
format	article
author	Yudai Tsuji Shinichi Yamaguchi Tomoyuki Nakamura Masaya Ikegawa
author_facet	Yudai Tsuji Shinichi Yamaguchi Tomoyuki Nakamura Masaya Ikegawa
author_sort	Yudai Tsuji
title	Mass Spectrometry Imaging (MSI) Delineates Thymus-Centric Metabolism In Vivo as an Effect of Systemic Administration of Dexamethasone
title_short	Mass Spectrometry Imaging (MSI) Delineates Thymus-Centric Metabolism In Vivo as an Effect of Systemic Administration of Dexamethasone
title_full	Mass Spectrometry Imaging (MSI) Delineates Thymus-Centric Metabolism In Vivo as an Effect of Systemic Administration of Dexamethasone
title_fullStr	Mass Spectrometry Imaging (MSI) Delineates Thymus-Centric Metabolism In Vivo as an Effect of Systemic Administration of Dexamethasone
title_full_unstemmed	Mass Spectrometry Imaging (MSI) Delineates Thymus-Centric Metabolism In Vivo as an Effect of Systemic Administration of Dexamethasone
title_sort	mass spectrometry imaging (msi) delineates thymus-centric metabolism in vivo as an effect of systemic administration of dexamethasone
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/62968cb0909a47fc86fbb4c4b141c5c9
work_keys_str_mv	AT yudaitsuji massspectrometryimagingmsidelineatesthymuscentricmetabolisminvivoasaneffectofsystemicadministrationofdexamethasone AT shinichiyamaguchi massspectrometryimagingmsidelineatesthymuscentricmetabolisminvivoasaneffectofsystemicadministrationofdexamethasone AT tomoyukinakamura massspectrometryimagingmsidelineatesthymuscentricmetabolisminvivoasaneffectofsystemicadministrationofdexamethasone AT masayaikegawa massspectrometryimagingmsidelineatesthymuscentricmetabolisminvivoasaneffectofsystemicadministrationofdexamethasone
_version_	1718413021180592128

Mass Spectrometry Imaging (MSI) Delineates Thymus-Centric Metabolism In Vivo as an Effect of Systemic Administration of Dexamethasone

Ejemplares similares