EXPRESS-DIAGNOSTIC OF RUBELLA INFECTION BY THE IMMUNOFLUORESCENT ASSAY USING MONOCLONAL ANTIBODIES TO PROTEIN E1 OF RUBELLA VIRUS

Abstract. The high rubella incidence rate in the Russian Federation and the low registration level of the rubella congenital syndrome which is not reflect the epidemic situation requiring optimization of the rubella diagnostics. In the present study the possibility of early detection of rubella viru...

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Autores principales:	A. N. Lisakov, F. G. Nagieva, E. P. Barkova, V. G. Niculina, N. V. Uminova, T. P. Antonova, A. U. Fedotov
Formato:	article
Lenguaje:	RU
Publicado:	Sankt-Peterburg : NIIÈM imeni Pastera 2014
Materias:	rapid culture method sensitive sells rubella virus monoclonal antibodies immune-fluorescent assay Infectious and parasitic diseases RC109-216
Acceso en línea:	https://doaj.org/article/6297cbf01c7540d2811db198c283f6ba
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spelling	oai:doaj.org-article:6297cbf01c7540d2811db198c283f6ba2021-11-22T07:09:46ZEXPRESS-DIAGNOSTIC OF RUBELLA INFECTION BY THE IMMUNOFLUORESCENT ASSAY USING MONOCLONAL ANTIBODIES TO PROTEIN E1 OF RUBELLA VIRUS2220-76192313-739810.15789/2220-7619-2012-4-735-740https://doaj.org/article/6297cbf01c7540d2811db198c283f6ba2014-07-01T00:00:00Zhttps://www.iimmun.ru/iimm/article/view/111https://doaj.org/toc/2220-7619https://doaj.org/toc/2313-7398Abstract. The high rubella incidence rate in the Russian Federation and the low registration level of the rubella congenital syndrome which is not reflect the epidemic situation requiring optimization of the rubella diagnostics. In the present study the possibility of early detection of rubella virus in case of inoculation of sensitive cell culture by the nasopharyngeal washouts with following detection of rubella antigen with highly specific monoclonal antibodies to the basic structural protein E1 of rubella virus using direct immunofluorescent method have been shown. Using of the rapid cell cultural method allows providing etiological diagnosis of high specificity within 5 hours after inoculation of virus to the sensitive cells Vero E6 or BHK-21-F.A. N. LisakovF. G. NagievaE. P. BarkovaV. G. NiculinaN. V. UminovaT. P. AntonovaA. U. FedotovSankt-Peterburg : NIIÈM imeni Pasteraarticlerapid culture methodsensitive sellsrubella virusmonoclonal antibodiesimmune-fluorescent assayInfectious and parasitic diseasesRC109-216RUInfekciâ i Immunitet, Vol 2, Iss 4, Pp 735-740 (2014)
institution	DOAJ
collection	DOAJ
language	RU
topic	rapid culture method sensitive sells rubella virus monoclonal antibodies immune-fluorescent assay Infectious and parasitic diseases RC109-216
spellingShingle	rapid culture method sensitive sells rubella virus monoclonal antibodies immune-fluorescent assay Infectious and parasitic diseases RC109-216 A. N. Lisakov F. G. Nagieva E. P. Barkova V. G. Niculina N. V. Uminova T. P. Antonova A. U. Fedotov EXPRESS-DIAGNOSTIC OF RUBELLA INFECTION BY THE IMMUNOFLUORESCENT ASSAY USING MONOCLONAL ANTIBODIES TO PROTEIN E1 OF RUBELLA VIRUS
description	Abstract. The high rubella incidence rate in the Russian Federation and the low registration level of the rubella congenital syndrome which is not reflect the epidemic situation requiring optimization of the rubella diagnostics. In the present study the possibility of early detection of rubella virus in case of inoculation of sensitive cell culture by the nasopharyngeal washouts with following detection of rubella antigen with highly specific monoclonal antibodies to the basic structural protein E1 of rubella virus using direct immunofluorescent method have been shown. Using of the rapid cell cultural method allows providing etiological diagnosis of high specificity within 5 hours after inoculation of virus to the sensitive cells Vero E6 or BHK-21-F.
format	article
author	A. N. Lisakov F. G. Nagieva E. P. Barkova V. G. Niculina N. V. Uminova T. P. Antonova A. U. Fedotov
author_facet	A. N. Lisakov F. G. Nagieva E. P. Barkova V. G. Niculina N. V. Uminova T. P. Antonova A. U. Fedotov
author_sort	A. N. Lisakov
title	EXPRESS-DIAGNOSTIC OF RUBELLA INFECTION BY THE IMMUNOFLUORESCENT ASSAY USING MONOCLONAL ANTIBODIES TO PROTEIN E1 OF RUBELLA VIRUS
title_short	EXPRESS-DIAGNOSTIC OF RUBELLA INFECTION BY THE IMMUNOFLUORESCENT ASSAY USING MONOCLONAL ANTIBODIES TO PROTEIN E1 OF RUBELLA VIRUS
title_full	EXPRESS-DIAGNOSTIC OF RUBELLA INFECTION BY THE IMMUNOFLUORESCENT ASSAY USING MONOCLONAL ANTIBODIES TO PROTEIN E1 OF RUBELLA VIRUS
title_fullStr	EXPRESS-DIAGNOSTIC OF RUBELLA INFECTION BY THE IMMUNOFLUORESCENT ASSAY USING MONOCLONAL ANTIBODIES TO PROTEIN E1 OF RUBELLA VIRUS
title_full_unstemmed	EXPRESS-DIAGNOSTIC OF RUBELLA INFECTION BY THE IMMUNOFLUORESCENT ASSAY USING MONOCLONAL ANTIBODIES TO PROTEIN E1 OF RUBELLA VIRUS
title_sort	express-diagnostic of rubella infection by the immunofluorescent assay using monoclonal antibodies to protein e1 of rubella virus
publisher	Sankt-Peterburg : NIIÈM imeni Pastera
publishDate	2014
url	https://doaj.org/article/6297cbf01c7540d2811db198c283f6ba
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EXPRESS-DIAGNOSTIC OF RUBELLA INFECTION BY THE IMMUNOFLUORESCENT ASSAY USING MONOCLONAL ANTIBODIES TO PROTEIN E1 OF RUBELLA VIRUS

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