Reconstitution of Monocyte Subsets and PD-L1 Expression but Not T Cell PD-1 Expression in Obstructive Sleep Apnea Patients upon PAP Therapy

Obstructive sleep apnea (OSA) is characterized by nocturnal breathing intermissions resulting in oxidative stress and eventually, a low-grade systemic inflammation. The study aimed to investigate the impact of positive airway pressure (PAP) therapy on the inflammatory milieu as measured by monocyte...

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Autores principales:	Christina Polasky, Armin Steffen, Kristin Loyal, Christian Lange, Karl-Ludwig Bruchhage, Ralph Pries
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	monocyte subsets PD-1/PD-L1 obstructive sleep apnea PAP therapy Biology (General) QH301-705.5 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/629e8daebe2647d9a9cbeac22b8d616a
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spelling	oai:doaj.org-article:629e8daebe2647d9a9cbeac22b8d616a2021-11-11T16:50:55ZReconstitution of Monocyte Subsets and PD-L1 Expression but Not T Cell PD-1 Expression in Obstructive Sleep Apnea Patients upon PAP Therapy10.3390/ijms2221113751422-00671661-6596https://doaj.org/article/629e8daebe2647d9a9cbeac22b8d616a2021-10-01T00:00:00Zhttps://www.mdpi.com/1422-0067/22/21/11375https://doaj.org/toc/1661-6596https://doaj.org/toc/1422-0067Obstructive sleep apnea (OSA) is characterized by nocturnal breathing intermissions resulting in oxidative stress and eventually, a low-grade systemic inflammation. The study aimed to investigate the impact of positive airway pressure (PAP) therapy on the inflammatory milieu as measured by monocyte and T cell phenotypic alterations. Participants were assessed for their OSA severity before PAP therapy and about six months later, including patient-reported outcome and therapy usage by telemetry readout. The distributions of the CD14/CD16-characterized monocyte subsets as well as the CD4/CD8-characterized effector T cell subsets with regard to their PD-1 and PD-L1 expression were analyzed by flow cytometry from blood samples. Data of 25 patients revealed a significant reconstitution of the monocyte subset distribution and a decrease in PD-L1 expression on pan-monocytes and CD8<sup>+</sup> T cells without an association to initial AHI and overweight. The PD-1 expression was still increased on T cell subsets, especially on CD4<sup>+</sup> TH17/22 cells. We conclude that PAP therapy might have a rapid effect on the monocyte phenotype and overall PD-L1 expression levels. However, T cell immune alterations especially on TH17/22 cells persist longer, indicating an ongoing disturbance of the adaptive immune system.Christina PolaskyArmin SteffenKristin LoyalChristian LangeKarl-Ludwig BruchhageRalph PriesMDPI AGarticlemonocyte subsetsPD-1/PD-L1obstructive sleep apneaPAP therapyBiology (General)QH301-705.5ChemistryQD1-999ENInternational Journal of Molecular Sciences, Vol 22, Iss 11375, p 11375 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	monocyte subsets PD-1/PD-L1 obstructive sleep apnea PAP therapy Biology (General) QH301-705.5 Chemistry QD1-999
spellingShingle	monocyte subsets PD-1/PD-L1 obstructive sleep apnea PAP therapy Biology (General) QH301-705.5 Chemistry QD1-999 Christina Polasky Armin Steffen Kristin Loyal Christian Lange Karl-Ludwig Bruchhage Ralph Pries Reconstitution of Monocyte Subsets and PD-L1 Expression but Not T Cell PD-1 Expression in Obstructive Sleep Apnea Patients upon PAP Therapy
description	Obstructive sleep apnea (OSA) is characterized by nocturnal breathing intermissions resulting in oxidative stress and eventually, a low-grade systemic inflammation. The study aimed to investigate the impact of positive airway pressure (PAP) therapy on the inflammatory milieu as measured by monocyte and T cell phenotypic alterations. Participants were assessed for their OSA severity before PAP therapy and about six months later, including patient-reported outcome and therapy usage by telemetry readout. The distributions of the CD14/CD16-characterized monocyte subsets as well as the CD4/CD8-characterized effector T cell subsets with regard to their PD-1 and PD-L1 expression were analyzed by flow cytometry from blood samples. Data of 25 patients revealed a significant reconstitution of the monocyte subset distribution and a decrease in PD-L1 expression on pan-monocytes and CD8<sup>+</sup> T cells without an association to initial AHI and overweight. The PD-1 expression was still increased on T cell subsets, especially on CD4<sup>+</sup> TH17/22 cells. We conclude that PAP therapy might have a rapid effect on the monocyte phenotype and overall PD-L1 expression levels. However, T cell immune alterations especially on TH17/22 cells persist longer, indicating an ongoing disturbance of the adaptive immune system.
format	article
author	Christina Polasky Armin Steffen Kristin Loyal Christian Lange Karl-Ludwig Bruchhage Ralph Pries
author_facet	Christina Polasky Armin Steffen Kristin Loyal Christian Lange Karl-Ludwig Bruchhage Ralph Pries
author_sort	Christina Polasky
title	Reconstitution of Monocyte Subsets and PD-L1 Expression but Not T Cell PD-1 Expression in Obstructive Sleep Apnea Patients upon PAP Therapy
title_short	Reconstitution of Monocyte Subsets and PD-L1 Expression but Not T Cell PD-1 Expression in Obstructive Sleep Apnea Patients upon PAP Therapy
title_full	Reconstitution of Monocyte Subsets and PD-L1 Expression but Not T Cell PD-1 Expression in Obstructive Sleep Apnea Patients upon PAP Therapy
title_fullStr	Reconstitution of Monocyte Subsets and PD-L1 Expression but Not T Cell PD-1 Expression in Obstructive Sleep Apnea Patients upon PAP Therapy
title_full_unstemmed	Reconstitution of Monocyte Subsets and PD-L1 Expression but Not T Cell PD-1 Expression in Obstructive Sleep Apnea Patients upon PAP Therapy
title_sort	reconstitution of monocyte subsets and pd-l1 expression but not t cell pd-1 expression in obstructive sleep apnea patients upon pap therapy
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/629e8daebe2647d9a9cbeac22b8d616a
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Reconstitution of Monocyte Subsets and PD-L1 Expression but Not T Cell PD-1 Expression in Obstructive Sleep Apnea Patients upon PAP Therapy

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