Untargeted metabolomics and transcriptomics identified glutathione metabolism disturbance and PCS and TMAO as potential biomarkers for ER stress in lung
Abstract Endoplasmic reticulum (ER) stress is a cellular state that results from the overload of unfolded/misfolded protein in the ER that, if not resolved properly, can lead to cell death. Both acute lung infections and chronic lung diseases have been found related to ER stress. Yet no study has be...
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2021
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oai:doaj.org-article:62a0aeb182ab46e390a062a80bac06792021-12-02T16:50:23ZUntargeted metabolomics and transcriptomics identified glutathione metabolism disturbance and PCS and TMAO as potential biomarkers for ER stress in lung10.1038/s41598-021-92779-82045-2322https://doaj.org/article/62a0aeb182ab46e390a062a80bac06792021-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-92779-8https://doaj.org/toc/2045-2322Abstract Endoplasmic reticulum (ER) stress is a cellular state that results from the overload of unfolded/misfolded protein in the ER that, if not resolved properly, can lead to cell death. Both acute lung infections and chronic lung diseases have been found related to ER stress. Yet no study has been presented integrating metabolomic and transcriptomic data from total lung in interpreting the pathogenic state of ER stress. Total mouse lungs were used to perform LC–MS and RNA sequencing in relevance to ER stress. Untargeted metabolomics revealed 16 metabolites of aberrant levels with statistical significance while transcriptomics revealed 1593 genes abnormally expressed. Enrichment results demonstrated the injury ER stress inflicted upon lung through the alteration of multiple critical pathways involving energy expenditure, signal transduction, and redox homeostasis. Ultimately, we have presented p-cresol sulfate (PCS) and trimethylamine N-oxide (TMAO) as two potential ER stress biomarkers. Glutathione metabolism stood out in both omics as a notably altered pathway that believed to take important roles in maintaining the redox homeostasis in the cells critical for the development and relief of ER stress, in consistence with the existing reports.Zijing WangPeng MaYisa WangBiyu HouCan ZhouHe TianBowen LiGuanghou ShuiXiuying YangGuifen QiangChengqian YinGuanhua DuNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021) |
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Medicine R Science Q Zijing Wang Peng Ma Yisa Wang Biyu Hou Can Zhou He Tian Bowen Li Guanghou Shui Xiuying Yang Guifen Qiang Chengqian Yin Guanhua Du Untargeted metabolomics and transcriptomics identified glutathione metabolism disturbance and PCS and TMAO as potential biomarkers for ER stress in lung |
| description |
Abstract Endoplasmic reticulum (ER) stress is a cellular state that results from the overload of unfolded/misfolded protein in the ER that, if not resolved properly, can lead to cell death. Both acute lung infections and chronic lung diseases have been found related to ER stress. Yet no study has been presented integrating metabolomic and transcriptomic data from total lung in interpreting the pathogenic state of ER stress. Total mouse lungs were used to perform LC–MS and RNA sequencing in relevance to ER stress. Untargeted metabolomics revealed 16 metabolites of aberrant levels with statistical significance while transcriptomics revealed 1593 genes abnormally expressed. Enrichment results demonstrated the injury ER stress inflicted upon lung through the alteration of multiple critical pathways involving energy expenditure, signal transduction, and redox homeostasis. Ultimately, we have presented p-cresol sulfate (PCS) and trimethylamine N-oxide (TMAO) as two potential ER stress biomarkers. Glutathione metabolism stood out in both omics as a notably altered pathway that believed to take important roles in maintaining the redox homeostasis in the cells critical for the development and relief of ER stress, in consistence with the existing reports. |
| format |
article |
| author |
Zijing Wang Peng Ma Yisa Wang Biyu Hou Can Zhou He Tian Bowen Li Guanghou Shui Xiuying Yang Guifen Qiang Chengqian Yin Guanhua Du |
| author_facet |
Zijing Wang Peng Ma Yisa Wang Biyu Hou Can Zhou He Tian Bowen Li Guanghou Shui Xiuying Yang Guifen Qiang Chengqian Yin Guanhua Du |
| author_sort |
Zijing Wang |
| title |
Untargeted metabolomics and transcriptomics identified glutathione metabolism disturbance and PCS and TMAO as potential biomarkers for ER stress in lung |
| title_short |
Untargeted metabolomics and transcriptomics identified glutathione metabolism disturbance and PCS and TMAO as potential biomarkers for ER stress in lung |
| title_full |
Untargeted metabolomics and transcriptomics identified glutathione metabolism disturbance and PCS and TMAO as potential biomarkers for ER stress in lung |
| title_fullStr |
Untargeted metabolomics and transcriptomics identified glutathione metabolism disturbance and PCS and TMAO as potential biomarkers for ER stress in lung |
| title_full_unstemmed |
Untargeted metabolomics and transcriptomics identified glutathione metabolism disturbance and PCS and TMAO as potential biomarkers for ER stress in lung |
| title_sort |
untargeted metabolomics and transcriptomics identified glutathione metabolism disturbance and pcs and tmao as potential biomarkers for er stress in lung |
| publisher |
Nature Portfolio |
| publishDate |
2021 |
| url |
https://doaj.org/article/62a0aeb182ab46e390a062a80bac0679 |
| work_keys_str_mv |
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