Induction of apoptosis and cell cycle arrest by chloroform fraction of Juniperus phoenicea and chemical constituents analysis
Different phytochemicals from various plant species exhibit promising medicinal properties against cancer. Juniperus phoenicea is a plant species that has been found to present medicinal properties. Herein, crude extract and fractions of J. phoenicea were examined to determine its anticancer propert...
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De Gruyter
2021
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oai:doaj.org-article:62a77a75f5be4366bfd94865c1d28ef02021-12-05T14:10:44ZInduction of apoptosis and cell cycle arrest by chloroform fraction of Juniperus phoenicea and chemical constituents analysis2391-542010.1515/chem-2021-0195https://doaj.org/article/62a77a75f5be4366bfd94865c1d28ef02021-02-01T00:00:00Zhttps://doi.org/10.1515/chem-2021-0195https://doaj.org/toc/2391-5420Different phytochemicals from various plant species exhibit promising medicinal properties against cancer. Juniperus phoenicea is a plant species that has been found to present medicinal properties. Herein, crude extract and fractions of J. phoenicea were examined to determine its anticancer properties against several cancer cells. The active fraction was chosen to assess its activity on cell cycle progression and apoptosis induction by annexin and propidium iodide (PI) biomarkers. Further, phytochemical screening for possible contents of active fraction using gas chromatography–mass spectrometry (GC-MS) analysis was conducted. It was demonstrated that cell proliferation was suppressed, and the MCF-7 cell line was the most sensitive to J. phoenicea chloroform fraction (JPCF), with the IC50 values of 24.5 μg/mL. The anti-proliferation activity of JPCF in MCF-7 cells was linked to the aggregation of cells in the G1 phase, increases in early and late apoptosis as well as necrotic cell death. Contents analysis of JPCF using GC-MS analysis identified 3-methyl-5-(2′,6′,6′-trimethylcyclohex-1′-enyl)-1-penten-3-ol (16.5%), methyl 8-oxooctanoate (15.61%), cubenol (13.48%), and 7-oxabicyclo [2.2.1] heptane (12.14%) as major constituents. Our present study provides clear evidence that J. phoenicea can inhibit cell proliferation, trigger cell cycle arrest, and induce apoptosis in tested cancer cells.Barnawi Ibrahim O.Nasr Fahd A.Noman Omar M.Alqahtani Ali S.Al-zharani MohammedAlotaibi Amal A.Daradka Haytham M.Al-Mishari Abdullah A.Alobaid Waleed AAlqahtani AbdulazizHerqash Rasheed N.De Gruyterarticlecupressaceaejuniperus phoeniceamtt assaycancergc-msChemistryQD1-999ENOpen Chemistry, Vol 19, Iss 1, Pp 119-127 (2021) |
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DOAJ |
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cupressaceae juniperus phoenicea mtt assay cancer gc-ms Chemistry QD1-999 |
| spellingShingle |
cupressaceae juniperus phoenicea mtt assay cancer gc-ms Chemistry QD1-999 Barnawi Ibrahim O. Nasr Fahd A. Noman Omar M. Alqahtani Ali S. Al-zharani Mohammed Alotaibi Amal A. Daradka Haytham M. Al-Mishari Abdullah A. Alobaid Waleed A Alqahtani Abdulaziz Herqash Rasheed N. Induction of apoptosis and cell cycle arrest by chloroform fraction of Juniperus phoenicea and chemical constituents analysis |
| description |
Different phytochemicals from various plant species exhibit promising medicinal properties against cancer. Juniperus phoenicea is a plant species that has been found to present medicinal properties. Herein, crude extract and fractions of J. phoenicea were examined to determine its anticancer properties against several cancer cells. The active fraction was chosen to assess its activity on cell cycle progression and apoptosis induction by annexin and propidium iodide (PI) biomarkers. Further, phytochemical screening for possible contents of active fraction using gas chromatography–mass spectrometry (GC-MS) analysis was conducted. It was demonstrated that cell proliferation was suppressed, and the MCF-7 cell line was the most sensitive to J. phoenicea chloroform fraction (JPCF), with the IC50 values of 24.5 μg/mL. The anti-proliferation activity of JPCF in MCF-7 cells was linked to the aggregation of cells in the G1 phase, increases in early and late apoptosis as well as necrotic cell death. Contents analysis of JPCF using GC-MS analysis identified 3-methyl-5-(2′,6′,6′-trimethylcyclohex-1′-enyl)-1-penten-3-ol (16.5%), methyl 8-oxooctanoate (15.61%), cubenol (13.48%), and 7-oxabicyclo [2.2.1] heptane (12.14%) as major constituents. Our present study provides clear evidence that J. phoenicea can inhibit cell proliferation, trigger cell cycle arrest, and induce apoptosis in tested cancer cells. |
| format |
article |
| author |
Barnawi Ibrahim O. Nasr Fahd A. Noman Omar M. Alqahtani Ali S. Al-zharani Mohammed Alotaibi Amal A. Daradka Haytham M. Al-Mishari Abdullah A. Alobaid Waleed A Alqahtani Abdulaziz Herqash Rasheed N. |
| author_facet |
Barnawi Ibrahim O. Nasr Fahd A. Noman Omar M. Alqahtani Ali S. Al-zharani Mohammed Alotaibi Amal A. Daradka Haytham M. Al-Mishari Abdullah A. Alobaid Waleed A Alqahtani Abdulaziz Herqash Rasheed N. |
| author_sort |
Barnawi Ibrahim O. |
| title |
Induction of apoptosis and cell cycle arrest by chloroform fraction of Juniperus phoenicea and chemical constituents analysis |
| title_short |
Induction of apoptosis and cell cycle arrest by chloroform fraction of Juniperus phoenicea and chemical constituents analysis |
| title_full |
Induction of apoptosis and cell cycle arrest by chloroform fraction of Juniperus phoenicea and chemical constituents analysis |
| title_fullStr |
Induction of apoptosis and cell cycle arrest by chloroform fraction of Juniperus phoenicea and chemical constituents analysis |
| title_full_unstemmed |
Induction of apoptosis and cell cycle arrest by chloroform fraction of Juniperus phoenicea and chemical constituents analysis |
| title_sort |
induction of apoptosis and cell cycle arrest by chloroform fraction of juniperus phoenicea and chemical constituents analysis |
| publisher |
De Gruyter |
| publishDate |
2021 |
| url |
https://doaj.org/article/62a77a75f5be4366bfd94865c1d28ef0 |
| work_keys_str_mv |
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