A nitrile hydratase in the eukaryote Monosiga brevicollis.

Bacterial nitrile hydratase (NHases) are important industrial catalysts and waste water remediation tools. In a global computational screening of conventional and metagenomic sequence data for NHases, we detected the two usually separated NHase subunits fused in one protein of the choanoflagellate M...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Konrad U Foerstner, Tobias Doerks, Jean Muller, Jeroen Raes, Peer Bork
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2008
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/62b2fe4f090045d2815a4ad93584fd57
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:62b2fe4f090045d2815a4ad93584fd57
record_format	dspace
spelling	oai:doaj.org-article:62b2fe4f090045d2815a4ad93584fd572021-11-25T06:18:07ZA nitrile hydratase in the eukaryote Monosiga brevicollis.1932-620310.1371/journal.pone.0003976https://doaj.org/article/62b2fe4f090045d2815a4ad93584fd572008-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/19096720/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Bacterial nitrile hydratase (NHases) are important industrial catalysts and waste water remediation tools. In a global computational screening of conventional and metagenomic sequence data for NHases, we detected the two usually separated NHase subunits fused in one protein of the choanoflagellate Monosiga brevicollis, a recently sequenced unicellular model organism from the closest sister group of Metazoa. This is the first time that an NHase is found in eukaryotes and the first time it is observed as a fusion protein. The presence of an intron, subunit fusion and expressed sequence tags covering parts of the gene exclude contamination and suggest a functional gene. Phylogenetic analyses and genomic context imply a probable ancient horizontal gene transfer (HGT) from proteobacteria. The newly discovered NHase might open biotechnological routes due to its unconventional structure, its new type of host and its apparent integration into eukaryotic protein networks.Konrad U FoerstnerTobias DoerksJean MullerJeroen RaesPeer BorkPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 3, Iss 12, p e3976 (2008)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Konrad U Foerstner Tobias Doerks Jean Muller Jeroen Raes Peer Bork A nitrile hydratase in the eukaryote Monosiga brevicollis.
description	Bacterial nitrile hydratase (NHases) are important industrial catalysts and waste water remediation tools. In a global computational screening of conventional and metagenomic sequence data for NHases, we detected the two usually separated NHase subunits fused in one protein of the choanoflagellate Monosiga brevicollis, a recently sequenced unicellular model organism from the closest sister group of Metazoa. This is the first time that an NHase is found in eukaryotes and the first time it is observed as a fusion protein. The presence of an intron, subunit fusion and expressed sequence tags covering parts of the gene exclude contamination and suggest a functional gene. Phylogenetic analyses and genomic context imply a probable ancient horizontal gene transfer (HGT) from proteobacteria. The newly discovered NHase might open biotechnological routes due to its unconventional structure, its new type of host and its apparent integration into eukaryotic protein networks.
format	article
author	Konrad U Foerstner Tobias Doerks Jean Muller Jeroen Raes Peer Bork
author_facet	Konrad U Foerstner Tobias Doerks Jean Muller Jeroen Raes Peer Bork
author_sort	Konrad U Foerstner
title	A nitrile hydratase in the eukaryote Monosiga brevicollis.
title_short	A nitrile hydratase in the eukaryote Monosiga brevicollis.
title_full	A nitrile hydratase in the eukaryote Monosiga brevicollis.
title_fullStr	A nitrile hydratase in the eukaryote Monosiga brevicollis.
title_full_unstemmed	A nitrile hydratase in the eukaryote Monosiga brevicollis.
title_sort	nitrile hydratase in the eukaryote monosiga brevicollis.
publisher	Public Library of Science (PLoS)
publishDate	2008
url	https://doaj.org/article/62b2fe4f090045d2815a4ad93584fd57
work_keys_str_mv	AT konradufoerstner anitrilehydrataseintheeukaryotemonosigabrevicollis AT tobiasdoerks anitrilehydrataseintheeukaryotemonosigabrevicollis AT jeanmuller anitrilehydrataseintheeukaryotemonosigabrevicollis AT jeroenraes anitrilehydrataseintheeukaryotemonosigabrevicollis AT peerbork anitrilehydrataseintheeukaryotemonosigabrevicollis AT konradufoerstner nitrilehydrataseintheeukaryotemonosigabrevicollis AT tobiasdoerks nitrilehydrataseintheeukaryotemonosigabrevicollis AT jeanmuller nitrilehydrataseintheeukaryotemonosigabrevicollis AT jeroenraes nitrilehydrataseintheeukaryotemonosigabrevicollis AT peerbork nitrilehydrataseintheeukaryotemonosigabrevicollis
_version_	1718413927754235904

A nitrile hydratase in the eukaryote Monosiga brevicollis.

Ejemplares similares