Evaluation of schistosomula crude antigen (SCA) as a diagnostic tool for Schistosoma mansoni in low endemic human population

Abstract The study aimed to determine the potential of schistosomula crude antigen (SCA) as a diagnostic target for anti-S. mansoni antibody detection. Cercariae were transformed into schistosomula, homogenized through sonication, and then centrifuged to obtain the SCA. SCA was evaluated using ELISA...

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Autores principales:	Oyetunde Timothy Oyeyemi, Camila Amormino Corsini, Gustavo Gonçalves, William de Castro Borges, Rafaella Fortini Queiroz Grenfell
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/62bce9e93c654e5f93974e0badcd33a8
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spelling	oai:doaj.org-article:62bce9e93c654e5f93974e0badcd33a82021-12-02T15:53:01ZEvaluation of schistosomula crude antigen (SCA) as a diagnostic tool for Schistosoma mansoni in low endemic human population10.1038/s41598-021-89929-32045-2322https://doaj.org/article/62bce9e93c654e5f93974e0badcd33a82021-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-89929-3https://doaj.org/toc/2045-2322Abstract The study aimed to determine the potential of schistosomula crude antigen (SCA) as a diagnostic target for anti-S. mansoni antibody detection. Cercariae were transformed into schistosomula, homogenized through sonication, and then centrifuged to obtain the SCA. SCA was evaluated using ELISA and dot blots immunoassays on 30 S. mansoni infected sera samples obtained from chronic patients and 30 non-infected humans’ sera samples. Either Kato-Katz or saline gradient method or both were employed as the diagnostic reference. Dot blots immunoassay was further performed on protein eluted from 10 to 12 kDa immunoreactive band identified by Western blot analysis. The area under the ROC curve was 0.95 (AUC 0.95, CI 0.88–1.01, p < 0.0001). The sensitivity and specificity of SCA-ELISA and dot blots assays were 96.67% and 86.67% respectively. The human IgG-specific response against SCA was significantly higher in S. mansoni infected individuals (OD = 0.678 ± 0.249) compared to the non-infected population (OD = 0.235 ± 0.136) (p < 0.0001). Our study showed that SCA and its 10–12 kDa component could be useful as diagnostic tools for chronic schistosomiasis.Oyetunde Timothy OyeyemiCamila Amormino CorsiniGustavo GonçalvesWilliam de Castro BorgesRafaella Fortini Queiroz GrenfellNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-8 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Oyetunde Timothy Oyeyemi Camila Amormino Corsini Gustavo Gonçalves William de Castro Borges Rafaella Fortini Queiroz Grenfell Evaluation of schistosomula crude antigen (SCA) as a diagnostic tool for Schistosoma mansoni in low endemic human population
description	Abstract The study aimed to determine the potential of schistosomula crude antigen (SCA) as a diagnostic target for anti-S. mansoni antibody detection. Cercariae were transformed into schistosomula, homogenized through sonication, and then centrifuged to obtain the SCA. SCA was evaluated using ELISA and dot blots immunoassays on 30 S. mansoni infected sera samples obtained from chronic patients and 30 non-infected humans’ sera samples. Either Kato-Katz or saline gradient method or both were employed as the diagnostic reference. Dot blots immunoassay was further performed on protein eluted from 10 to 12 kDa immunoreactive band identified by Western blot analysis. The area under the ROC curve was 0.95 (AUC 0.95, CI 0.88–1.01, p < 0.0001). The sensitivity and specificity of SCA-ELISA and dot blots assays were 96.67% and 86.67% respectively. The human IgG-specific response against SCA was significantly higher in S. mansoni infected individuals (OD = 0.678 ± 0.249) compared to the non-infected population (OD = 0.235 ± 0.136) (p < 0.0001). Our study showed that SCA and its 10–12 kDa component could be useful as diagnostic tools for chronic schistosomiasis.
format	article
author	Oyetunde Timothy Oyeyemi Camila Amormino Corsini Gustavo Gonçalves William de Castro Borges Rafaella Fortini Queiroz Grenfell
author_facet	Oyetunde Timothy Oyeyemi Camila Amormino Corsini Gustavo Gonçalves William de Castro Borges Rafaella Fortini Queiroz Grenfell
author_sort	Oyetunde Timothy Oyeyemi
title	Evaluation of schistosomula crude antigen (SCA) as a diagnostic tool for Schistosoma mansoni in low endemic human population
title_short	Evaluation of schistosomula crude antigen (SCA) as a diagnostic tool for Schistosoma mansoni in low endemic human population
title_full	Evaluation of schistosomula crude antigen (SCA) as a diagnostic tool for Schistosoma mansoni in low endemic human population
title_fullStr	Evaluation of schistosomula crude antigen (SCA) as a diagnostic tool for Schistosoma mansoni in low endemic human population
title_full_unstemmed	Evaluation of schistosomula crude antigen (SCA) as a diagnostic tool for Schistosoma mansoni in low endemic human population
title_sort	evaluation of schistosomula crude antigen (sca) as a diagnostic tool for schistosoma mansoni in low endemic human population
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/62bce9e93c654e5f93974e0badcd33a8
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Evaluation of schistosomula crude antigen (SCA) as a diagnostic tool for Schistosoma mansoni in low endemic human population

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