Transcriptome sequencing identified the ceRNA network associated with recurrent spontaneous abortion

Abstract Background Recurrent spontaneous abortion (RSA) is one of the common complication of pregnancy, bringing heavy burden to the patients and their families. The study aimed to explore the lncRNA-miRNA-mRNA network associated with recurrent spontaneous abortion. Methods By transcriptome sequenc...

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Autores principales:	Yong Huang, Jiayuan Hao, Yuan Liao, Lihua Zhou, Kaiju Wang, Hui Zou, Ying Hu, Juan Li
Formato:	article
Lenguaje:	EN
Publicado:	BMC 2021
Materias:	Recurrent spontaneous abortion Transcriptome sequencing lncRNA ceRNA network Internal medicine RC31-1245 Genetics QH426-470
Acceso en línea:	https://doaj.org/article/62bf9ffa34414a4e84f46752ac6477b3
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spelling	oai:doaj.org-article:62bf9ffa34414a4e84f46752ac6477b32021-11-28T12:06:02ZTranscriptome sequencing identified the ceRNA network associated with recurrent spontaneous abortion10.1186/s12920-021-01125-41755-8794https://doaj.org/article/62bf9ffa34414a4e84f46752ac6477b32021-11-01T00:00:00Zhttps://doi.org/10.1186/s12920-021-01125-4https://doaj.org/toc/1755-8794Abstract Background Recurrent spontaneous abortion (RSA) is one of the common complication of pregnancy, bringing heavy burden to the patients and their families. The study aimed to explore the lncRNA-miRNA-mRNA network associated with recurrent spontaneous abortion. Methods By transcriptome sequencing, we detected differences in lncRNA, miRNA and mRNA expression in villus tissue samples collected from 3 patients with RSA and 3 normal abortion patients. Differentially expressed lncRNAs, miRNAs and genes (DELs, DEMs and DEGs, respectively) were identified, and Geno Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses were used to determine the functions of DELs and DEGs, which were analysed by Fisher’s test. We also observed the regulatory relationships between miRNA-mRNA and lncRNA-miRNA by Cytoscape 3.6.1. Results The results showed that 1008 DELs (523 upregulated and 485 downregulated), 475 DEGs (201 upregulated and 274 downregulated) and 37 DEMs (15 upregulated and 22 downregulated) were identified. And we also constructed a novel lncRNA-related ceRNA network containing 31 lncRNAs, 1 miRNA (hsa-miR-210-5p) and 3 genes (NTNG2, GRIA1 and AQP1). Conclusions lncRNA-related ceRNA network containing 31 lncRNAs, 1 miRNA (hsa-miR-210-5p) and 3 mRNAs (NTNG2, GRIA1 and AQP1) was constructed. The results may provide a basic theory for elucidating the mechanism underlying RSA.Yong HuangJiayuan HaoYuan LiaoLihua ZhouKaiju WangHui ZouYing HuJuan LiBMCarticleRecurrent spontaneous abortionTranscriptome sequencinglncRNAceRNA networkInternal medicineRC31-1245GeneticsQH426-470ENBMC Medical Genomics, Vol 14, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Recurrent spontaneous abortion Transcriptome sequencing lncRNA ceRNA network Internal medicine RC31-1245 Genetics QH426-470
spellingShingle	Recurrent spontaneous abortion Transcriptome sequencing lncRNA ceRNA network Internal medicine RC31-1245 Genetics QH426-470 Yong Huang Jiayuan Hao Yuan Liao Lihua Zhou Kaiju Wang Hui Zou Ying Hu Juan Li Transcriptome sequencing identified the ceRNA network associated with recurrent spontaneous abortion
description	Abstract Background Recurrent spontaneous abortion (RSA) is one of the common complication of pregnancy, bringing heavy burden to the patients and their families. The study aimed to explore the lncRNA-miRNA-mRNA network associated with recurrent spontaneous abortion. Methods By transcriptome sequencing, we detected differences in lncRNA, miRNA and mRNA expression in villus tissue samples collected from 3 patients with RSA and 3 normal abortion patients. Differentially expressed lncRNAs, miRNAs and genes (DELs, DEMs and DEGs, respectively) were identified, and Geno Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses were used to determine the functions of DELs and DEGs, which were analysed by Fisher’s test. We also observed the regulatory relationships between miRNA-mRNA and lncRNA-miRNA by Cytoscape 3.6.1. Results The results showed that 1008 DELs (523 upregulated and 485 downregulated), 475 DEGs (201 upregulated and 274 downregulated) and 37 DEMs (15 upregulated and 22 downregulated) were identified. And we also constructed a novel lncRNA-related ceRNA network containing 31 lncRNAs, 1 miRNA (hsa-miR-210-5p) and 3 genes (NTNG2, GRIA1 and AQP1). Conclusions lncRNA-related ceRNA network containing 31 lncRNAs, 1 miRNA (hsa-miR-210-5p) and 3 mRNAs (NTNG2, GRIA1 and AQP1) was constructed. The results may provide a basic theory for elucidating the mechanism underlying RSA.
format	article
author	Yong Huang Jiayuan Hao Yuan Liao Lihua Zhou Kaiju Wang Hui Zou Ying Hu Juan Li
author_facet	Yong Huang Jiayuan Hao Yuan Liao Lihua Zhou Kaiju Wang Hui Zou Ying Hu Juan Li
author_sort	Yong Huang
title	Transcriptome sequencing identified the ceRNA network associated with recurrent spontaneous abortion
title_short	Transcriptome sequencing identified the ceRNA network associated with recurrent spontaneous abortion
title_full	Transcriptome sequencing identified the ceRNA network associated with recurrent spontaneous abortion
title_fullStr	Transcriptome sequencing identified the ceRNA network associated with recurrent spontaneous abortion
title_full_unstemmed	Transcriptome sequencing identified the ceRNA network associated with recurrent spontaneous abortion
title_sort	transcriptome sequencing identified the cerna network associated with recurrent spontaneous abortion
publisher	BMC
publishDate	2021
url	https://doaj.org/article/62bf9ffa34414a4e84f46752ac6477b3
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Transcriptome sequencing identified the ceRNA network associated with recurrent spontaneous abortion

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