In-vitro effect of the methanolic extract of Momordica charantia on hatching of eggs of Haemonchus sp.

Background: Endoparasitism, particularly infections by gastrointestinal nematodes (e.g., Haemochus sp.), has been associated with economic losses within sheep production systems in tropical regions. Thus, implementing therapeutic alternatives that are environmentally sustainable is essential for pa...

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Autores principales:	Dumar Alexander Jaramillo Hernández, Adolfo VÁSQUEZ TRUJILLO, Lida Carolina LESMES RODRÍGUEZ
Formato:	article
Lenguaje:	EN
Publicado:	Universidad de Antioquia 2021
Materias:	haemonchosis phytotherapy sheep farming Food processing and manufacture TP368-456 Pharmaceutical industry HD9665-9675
Acceso en línea:	https://doaj.org/article/62c282b37d6e4c6ea956732bb281b2d6
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spelling	oai:doaj.org-article:62c282b37d6e4c6ea956732bb281b2d62021-12-02T17:56:04ZIn-vitro effect of the methanolic extract of Momordica charantia on hatching of eggs of Haemonchus sp.10.17533/udea.vitae.v28n1a3452150121-40042145-2660https://doaj.org/article/62c282b37d6e4c6ea956732bb281b2d62021-04-01T00:00:00Zhttps://revistas.udea.edu.co/index.php/vitae/article/view/345215https://doaj.org/toc/0121-4004https://doaj.org/toc/2145-2660 Background: Endoparasitism, particularly infections by gastrointestinal nematodes (e.g., Haemochus sp.), has been associated with economic losses within sheep production systems in tropical regions. Thus, implementing therapeutic alternatives that are environmentally sustainable is essential for parasite integral control programs. Objectives: Evaluate in-vitro the effect of Momordica charantia methanolic extract on the Haemonchus sp. eggs' hatching process. Methods: Nematode eggs were retrieved from experimentally infected sheep and exposed to 10, 20, 40, 80, and 160 mg/mL of methanolic extract of M. charantia. Hatching percentages were recorded from five replicates, and CL50 and CL90 were estimated through Probit regression analysis. Results: A significant effect on the hatching percentages were observed, from 24.2% up to 84.6% inhibition (p<0.05). The LC50 and LC90 estimated were 52.2 mg/mL (95%CI 37.87-63.22) and 201.45 mg/mL (95%CI 186.01-221.89), respectively. Utilizing a preliminary phytochemical analysis, potential antihelmintic compounds such as alkaloid, triterpenes, and anthracenic glycosides groups were identified in the methanolic extract. Conclusions: In the in-vitro test, the methanolic extract of M. charantia was effective in inhibiting the hatching of Haemonchus sp., which is important to promote other bio-guided fractionation studies of this plant on different life stages of H. contortus, this being a plant species widely adapted to the conditions of the piedmont (foothills) of Meta, Colombia. Dumar Alexander Jaramillo HernándezAdolfo VÁSQUEZ TRUJILLOLida Carolina LESMES RODRÍGUEZUniversidad de Antioquiaarticlehaemonchosisphytotherapysheep farmingFood processing and manufactureTP368-456Pharmaceutical industryHD9665-9675ENVitae, Vol 28, Iss 01 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	haemonchosis phytotherapy sheep farming Food processing and manufacture TP368-456 Pharmaceutical industry HD9665-9675
spellingShingle	haemonchosis phytotherapy sheep farming Food processing and manufacture TP368-456 Pharmaceutical industry HD9665-9675 Dumar Alexander Jaramillo Hernández Adolfo VÁSQUEZ TRUJILLO Lida Carolina LESMES RODRÍGUEZ In-vitro effect of the methanolic extract of Momordica charantia on hatching of eggs of Haemonchus sp.
description	Background: Endoparasitism, particularly infections by gastrointestinal nematodes (e.g., Haemochus sp.), has been associated with economic losses within sheep production systems in tropical regions. Thus, implementing therapeutic alternatives that are environmentally sustainable is essential for parasite integral control programs. Objectives: Evaluate in-vitro the effect of Momordica charantia methanolic extract on the Haemonchus sp. eggs' hatching process. Methods: Nematode eggs were retrieved from experimentally infected sheep and exposed to 10, 20, 40, 80, and 160 mg/mL of methanolic extract of M. charantia. Hatching percentages were recorded from five replicates, and CL50 and CL90 were estimated through Probit regression analysis. Results: A significant effect on the hatching percentages were observed, from 24.2% up to 84.6% inhibition (p<0.05). The LC50 and LC90 estimated were 52.2 mg/mL (95%CI 37.87-63.22) and 201.45 mg/mL (95%CI 186.01-221.89), respectively. Utilizing a preliminary phytochemical analysis, potential antihelmintic compounds such as alkaloid, triterpenes, and anthracenic glycosides groups were identified in the methanolic extract. Conclusions: In the in-vitro test, the methanolic extract of M. charantia was effective in inhibiting the hatching of Haemonchus sp., which is important to promote other bio-guided fractionation studies of this plant on different life stages of H. contortus, this being a plant species widely adapted to the conditions of the piedmont (foothills) of Meta, Colombia.
format	article
author	Dumar Alexander Jaramillo Hernández Adolfo VÁSQUEZ TRUJILLO Lida Carolina LESMES RODRÍGUEZ
author_facet	Dumar Alexander Jaramillo Hernández Adolfo VÁSQUEZ TRUJILLO Lida Carolina LESMES RODRÍGUEZ
author_sort	Dumar Alexander Jaramillo Hernández
title	In-vitro effect of the methanolic extract of Momordica charantia on hatching of eggs of Haemonchus sp.
title_short	In-vitro effect of the methanolic extract of Momordica charantia on hatching of eggs of Haemonchus sp.
title_full	In-vitro effect of the methanolic extract of Momordica charantia on hatching of eggs of Haemonchus sp.
title_fullStr	In-vitro effect of the methanolic extract of Momordica charantia on hatching of eggs of Haemonchus sp.
title_full_unstemmed	In-vitro effect of the methanolic extract of Momordica charantia on hatching of eggs of Haemonchus sp.
title_sort	in-vitro effect of the methanolic extract of momordica charantia on hatching of eggs of haemonchus sp.
publisher	Universidad de Antioquia
publishDate	2021
url	https://doaj.org/article/62c282b37d6e4c6ea956732bb281b2d6
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In-vitro effect of the methanolic extract of Momordica charantia on hatching of eggs of Haemonchus sp.

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