Serotonin is a key factor for mouse red blood cell survival.

Serotonin (5-HT) is a monoamine originally purified from blood as a vasoactive agent. In nonneuronal tissues, its presence is linked with the expression of tryptophan hydroxylase 1 (TPH1) that catalyzes the rate-limiting step of its synthesis. Targeted disruption in mice of the TPH1 gene results in...

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Autores principales:	Pascal Amireault, Elisa Bayard, Jean-Marie Launay, David Sibon, Caroline Le Van Kim, Yves Colin, Michel Dy, Olivier Hermine, Francine Côté
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/62c7ab21352549c4bad1d876c5cbb850
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spelling	oai:doaj.org-article:62c7ab21352549c4bad1d876c5cbb8502021-11-18T08:41:36ZSerotonin is a key factor for mouse red blood cell survival.1932-620310.1371/journal.pone.0083010https://doaj.org/article/62c7ab21352549c4bad1d876c5cbb8502013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24358245/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Serotonin (5-HT) is a monoamine originally purified from blood as a vasoactive agent. In nonneuronal tissues, its presence is linked with the expression of tryptophan hydroxylase 1 (TPH1) that catalyzes the rate-limiting step of its synthesis. Targeted disruption in mice of the TPH1 gene results in very low levels of circulating 5-HT. Previous analysis of the TPH1 knockout (TPH1(-/-)) mouse revealed that they develop a phenotype of macrocytic anemia with a reduced half-life of their circulating red blood cells (RBC). In this study, to establish whether the observed reduced half-life of TPH1(-/-) RBC is an intrinsic or an extrinsic characteristic, we compared their survival to RBC isolated from wild-type mice. Both in vivo and in vitro data converge to demonstrate an extrinsic protective effect of 5-HT since presence of 5-HT in the RBC environment protects RBC from senescence. The protective effect played by 5-HT is not mediated through activation of a classical pharmacological pathway as no 5-HT receptors were detected on isolated RBC. Rather, 5-HT acts as an effective antioxidant since reduction of 5-HT circulating levels are associated with a decrease in the plasma antioxidant capacity. We further demonstrate a link between oxidation and the removal of damaged RBC following transfusion, as supplementation with 5-HT improves RBC post-transfusion survival in a mouse model of blood banking.Pascal AmireaultElisa BayardJean-Marie LaunayDavid SibonCaroline Le Van KimYves ColinMichel DyOlivier HermineFrancine CôtéPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 12, p e83010 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Pascal Amireault Elisa Bayard Jean-Marie Launay David Sibon Caroline Le Van Kim Yves Colin Michel Dy Olivier Hermine Francine Côté Serotonin is a key factor for mouse red blood cell survival.
description	Serotonin (5-HT) is a monoamine originally purified from blood as a vasoactive agent. In nonneuronal tissues, its presence is linked with the expression of tryptophan hydroxylase 1 (TPH1) that catalyzes the rate-limiting step of its synthesis. Targeted disruption in mice of the TPH1 gene results in very low levels of circulating 5-HT. Previous analysis of the TPH1 knockout (TPH1(-/-)) mouse revealed that they develop a phenotype of macrocytic anemia with a reduced half-life of their circulating red blood cells (RBC). In this study, to establish whether the observed reduced half-life of TPH1(-/-) RBC is an intrinsic or an extrinsic characteristic, we compared their survival to RBC isolated from wild-type mice. Both in vivo and in vitro data converge to demonstrate an extrinsic protective effect of 5-HT since presence of 5-HT in the RBC environment protects RBC from senescence. The protective effect played by 5-HT is not mediated through activation of a classical pharmacological pathway as no 5-HT receptors were detected on isolated RBC. Rather, 5-HT acts as an effective antioxidant since reduction of 5-HT circulating levels are associated with a decrease in the plasma antioxidant capacity. We further demonstrate a link between oxidation and the removal of damaged RBC following transfusion, as supplementation with 5-HT improves RBC post-transfusion survival in a mouse model of blood banking.
format	article
author	Pascal Amireault Elisa Bayard Jean-Marie Launay David Sibon Caroline Le Van Kim Yves Colin Michel Dy Olivier Hermine Francine Côté
author_facet	Pascal Amireault Elisa Bayard Jean-Marie Launay David Sibon Caroline Le Van Kim Yves Colin Michel Dy Olivier Hermine Francine Côté
author_sort	Pascal Amireault
title	Serotonin is a key factor for mouse red blood cell survival.
title_short	Serotonin is a key factor for mouse red blood cell survival.
title_full	Serotonin is a key factor for mouse red blood cell survival.
title_fullStr	Serotonin is a key factor for mouse red blood cell survival.
title_full_unstemmed	Serotonin is a key factor for mouse red blood cell survival.
title_sort	serotonin is a key factor for mouse red blood cell survival.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/62c7ab21352549c4bad1d876c5cbb850
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Serotonin is a key factor for mouse red blood cell survival.

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