Mg, K-containing microparticle: A possible active principle of a culture extract produced by a microbial consortium.

A synthetic microbial consortium called Effective Microorganisms (EM) consists mainly of photosynthetic bacteria, lactic acid bacteria and yeast. Various effects of EM∙XGOLD, a health drink produced by EM, on life cycle of Dictyostelium discoideum were described previously. Here, we report our attem...

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Autores principales:	Toru Higashinakagawa, Haruhisa Kikuchi, Hidekazu Kuwayama
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/62d2d52075824e149094b69d2201c6f0
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spelling	oai:doaj.org-article:62d2d52075824e149094b69d2201c6f02021-12-02T20:04:29ZMg, K-containing microparticle: A possible active principle of a culture extract produced by a microbial consortium.1932-620310.1371/journal.pone.0259297https://doaj.org/article/62d2d52075824e149094b69d2201c6f02021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0259297https://doaj.org/toc/1932-6203A synthetic microbial consortium called Effective Microorganisms (EM) consists mainly of photosynthetic bacteria, lactic acid bacteria and yeast. Various effects of EM∙XGOLD, a health drink produced by EM, on life cycle of Dictyostelium discoideum were described previously. Here, we report our attempt to identify the active principle, termed EMF, that brought about the observed effects. Throughout the purification processes, the presence of the active principle was monitored by promoted fruiting body formation. By liquid-liquid separation the activity was recovered in aqueous phase, which, after concentration, was further subjected to reverse-phase column chromatography. No activity was detected in any eluant, while almost all the activity was recovered in residual insoluble material. The application of conventional organic chemistry procedures to the residual fraction did not lead to any informative results. Acid treatment of the insoluble material produced air bubbles, suggesting it to be composed of some inorganic carbonate. Viewed under scanning electronmicroscope, the residue revealed spherical particles of μm size range. Energy Dispersive X-ray (EDX) Spectroscopy pointed to the existence, on the surface of the particles, of magnesium and, to a certain extent, of potassium. In separate experiments, acid treatment and alkali neutralization of EM∙XGOLD completely wiped out the stimulatory activity of fruiting body formation. These lines of evidence indicate these Mg, K-containing microparticles to be an active principle of EM culture extract. How these particles exert their effect is currently under intensive investigation.Toru HigashinakagawaHaruhisa KikuchiHidekazu KuwayamaPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 11, p e0259297 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Toru Higashinakagawa Haruhisa Kikuchi Hidekazu Kuwayama Mg, K-containing microparticle: A possible active principle of a culture extract produced by a microbial consortium.
description	A synthetic microbial consortium called Effective Microorganisms (EM) consists mainly of photosynthetic bacteria, lactic acid bacteria and yeast. Various effects of EM∙XGOLD, a health drink produced by EM, on life cycle of Dictyostelium discoideum were described previously. Here, we report our attempt to identify the active principle, termed EMF, that brought about the observed effects. Throughout the purification processes, the presence of the active principle was monitored by promoted fruiting body formation. By liquid-liquid separation the activity was recovered in aqueous phase, which, after concentration, was further subjected to reverse-phase column chromatography. No activity was detected in any eluant, while almost all the activity was recovered in residual insoluble material. The application of conventional organic chemistry procedures to the residual fraction did not lead to any informative results. Acid treatment of the insoluble material produced air bubbles, suggesting it to be composed of some inorganic carbonate. Viewed under scanning electronmicroscope, the residue revealed spherical particles of μm size range. Energy Dispersive X-ray (EDX) Spectroscopy pointed to the existence, on the surface of the particles, of magnesium and, to a certain extent, of potassium. In separate experiments, acid treatment and alkali neutralization of EM∙XGOLD completely wiped out the stimulatory activity of fruiting body formation. These lines of evidence indicate these Mg, K-containing microparticles to be an active principle of EM culture extract. How these particles exert their effect is currently under intensive investigation.
format	article
author	Toru Higashinakagawa Haruhisa Kikuchi Hidekazu Kuwayama
author_facet	Toru Higashinakagawa Haruhisa Kikuchi Hidekazu Kuwayama
author_sort	Toru Higashinakagawa
title	Mg, K-containing microparticle: A possible active principle of a culture extract produced by a microbial consortium.
title_short	Mg, K-containing microparticle: A possible active principle of a culture extract produced by a microbial consortium.
title_full	Mg, K-containing microparticle: A possible active principle of a culture extract produced by a microbial consortium.
title_fullStr	Mg, K-containing microparticle: A possible active principle of a culture extract produced by a microbial consortium.
title_full_unstemmed	Mg, K-containing microparticle: A possible active principle of a culture extract produced by a microbial consortium.
title_sort	mg, k-containing microparticle: a possible active principle of a culture extract produced by a microbial consortium.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/62d2d52075824e149094b69d2201c6f0
work_keys_str_mv	AT toruhigashinakagawa mgkcontainingmicroparticleapossibleactiveprincipleofacultureextractproducedbyamicrobialconsortium AT haruhisakikuchi mgkcontainingmicroparticleapossibleactiveprincipleofacultureextractproducedbyamicrobialconsortium AT hidekazukuwayama mgkcontainingmicroparticleapossibleactiveprincipleofacultureextractproducedbyamicrobialconsortium
_version_	1718375605618081792

Mg, K-containing microparticle: A possible active principle of a culture extract produced by a microbial consortium.

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