Label Free Detection of Sensitive Mid-Infrared Biomarkers of Glomerulonephritis in Urine Using Fourier Transform Infrared Spectroscopy

Abstract More reliable biomarkers using near-patient technologies are needed to improve early diagnosis and intervention for patients with renal disease. Infrared (IR) vibrational spectroscopy/microspectroscopy is an established analytical method that was first used in biomedical research over 20 ye...

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Autores principales:	Mei-Ching Yu, Peter Rich, Liberty Foreman, Jennifer Smith, Mei-Shiuan Yu, Anisha Tanna, Vinod Dibbur, Robert Unwin, Frederick W. K. Tam
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/62d6f999133349a4ac62dbdefdab819d
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spelling	oai:doaj.org-article:62d6f999133349a4ac62dbdefdab819d2021-12-02T11:53:09ZLabel Free Detection of Sensitive Mid-Infrared Biomarkers of Glomerulonephritis in Urine Using Fourier Transform Infrared Spectroscopy10.1038/s41598-017-04774-72045-2322https://doaj.org/article/62d6f999133349a4ac62dbdefdab819d2017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-04774-7https://doaj.org/toc/2045-2322Abstract More reliable biomarkers using near-patient technologies are needed to improve early diagnosis and intervention for patients with renal disease. Infrared (IR) vibrational spectroscopy/microspectroscopy is an established analytical method that was first used in biomedical research over 20 years ago. With the advances in instrumentation, computational and mathematical techniques, this technology has now been applied to a variety of diseases; however, applications in nephrology are just beginning to emerge. In the present study, we used attenuated total reflectance Fourier transform infrared (ATR-FTIR) spectroscopy to analyze urine samples collected from rodent models of inflammatory glomerulonephritis (GN) as well as from patients with crescentic GN, with the aim of identifying potential renal biomarkers; several characteristic mid-IR spectral markers were identified in urine samples. Specifically, a 1545 cm−1 band increased in intensity with the progression and severity of GN in rats, mice and humans. Furthermore, its intensity declined significantly in response to corticosteroid treatment in nephritic rats. In conclusion, our results suggest that specific urinary FTIR biomarkers may provide a rapid, sensitive and novel non-invasive means of diagnosing inflammatory forms of GN, and for real-time monitoring of progress, and response to treatment.Mei-Ching YuPeter RichLiberty ForemanJennifer SmithMei-Shiuan YuAnisha TannaVinod DibburRobert UnwinFrederick W. K. TamNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-12 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Mei-Ching Yu Peter Rich Liberty Foreman Jennifer Smith Mei-Shiuan Yu Anisha Tanna Vinod Dibbur Robert Unwin Frederick W. K. Tam Label Free Detection of Sensitive Mid-Infrared Biomarkers of Glomerulonephritis in Urine Using Fourier Transform Infrared Spectroscopy
description	Abstract More reliable biomarkers using near-patient technologies are needed to improve early diagnosis and intervention for patients with renal disease. Infrared (IR) vibrational spectroscopy/microspectroscopy is an established analytical method that was first used in biomedical research over 20 years ago. With the advances in instrumentation, computational and mathematical techniques, this technology has now been applied to a variety of diseases; however, applications in nephrology are just beginning to emerge. In the present study, we used attenuated total reflectance Fourier transform infrared (ATR-FTIR) spectroscopy to analyze urine samples collected from rodent models of inflammatory glomerulonephritis (GN) as well as from patients with crescentic GN, with the aim of identifying potential renal biomarkers; several characteristic mid-IR spectral markers were identified in urine samples. Specifically, a 1545 cm−1 band increased in intensity with the progression and severity of GN in rats, mice and humans. Furthermore, its intensity declined significantly in response to corticosteroid treatment in nephritic rats. In conclusion, our results suggest that specific urinary FTIR biomarkers may provide a rapid, sensitive and novel non-invasive means of diagnosing inflammatory forms of GN, and for real-time monitoring of progress, and response to treatment.
format	article
author	Mei-Ching Yu Peter Rich Liberty Foreman Jennifer Smith Mei-Shiuan Yu Anisha Tanna Vinod Dibbur Robert Unwin Frederick W. K. Tam
author_facet	Mei-Ching Yu Peter Rich Liberty Foreman Jennifer Smith Mei-Shiuan Yu Anisha Tanna Vinod Dibbur Robert Unwin Frederick W. K. Tam
author_sort	Mei-Ching Yu
title	Label Free Detection of Sensitive Mid-Infrared Biomarkers of Glomerulonephritis in Urine Using Fourier Transform Infrared Spectroscopy
title_short	Label Free Detection of Sensitive Mid-Infrared Biomarkers of Glomerulonephritis in Urine Using Fourier Transform Infrared Spectroscopy
title_full	Label Free Detection of Sensitive Mid-Infrared Biomarkers of Glomerulonephritis in Urine Using Fourier Transform Infrared Spectroscopy
title_fullStr	Label Free Detection of Sensitive Mid-Infrared Biomarkers of Glomerulonephritis in Urine Using Fourier Transform Infrared Spectroscopy
title_full_unstemmed	Label Free Detection of Sensitive Mid-Infrared Biomarkers of Glomerulonephritis in Urine Using Fourier Transform Infrared Spectroscopy
title_sort	label free detection of sensitive mid-infrared biomarkers of glomerulonephritis in urine using fourier transform infrared spectroscopy
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/62d6f999133349a4ac62dbdefdab819d
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Label Free Detection of Sensitive Mid-Infrared Biomarkers of Glomerulonephritis in Urine Using Fourier Transform Infrared Spectroscopy

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