Ribo-attenuators: novel elements for reliable and modular riboswitch engineering

Abstract Riboswitches are structural genetic regulatory elements that directly couple the sensing of small molecules to gene expression. They have considerable potential for applications throughout synthetic biology and bio-manufacturing as they are able to sense a wide range of small molecules and...

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Autores principales:	Thomas Folliard, Barbara Mertins, Harrison Steel, Thomas P. Prescott, Thomas Newport, Christopher W. Jones, George Wadhams, Travis Bayer, Judith P. Armitage, Antonis Papachristodoulou, Lynn J. Rothschild
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/62d7c8bca79445f6a7fad7f630e03f74
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spelling	oai:doaj.org-article:62d7c8bca79445f6a7fad7f630e03f742021-12-02T15:05:05ZRibo-attenuators: novel elements for reliable and modular riboswitch engineering10.1038/s41598-017-04093-x2045-2322https://doaj.org/article/62d7c8bca79445f6a7fad7f630e03f742017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-04093-xhttps://doaj.org/toc/2045-2322Abstract Riboswitches are structural genetic regulatory elements that directly couple the sensing of small molecules to gene expression. They have considerable potential for applications throughout synthetic biology and bio-manufacturing as they are able to sense a wide range of small molecules and regulate gene expression in response. Despite over a decade of research they have yet to reach this considerable potential as they cannot yet be treated as modular components. This is due to several limitations including sensitivity to changes in genetic context, low tunability, and variability in performance. To overcome the associated difficulties with riboswitches, we have designed and introduced a novel genetic element called a ribo-attenuator in Bacteria. This genetic element allows for predictable tuning, insulation from contextual changes, and a reduction in expression variation. Ribo-attenuators allow riboswitches to be treated as truly modular and tunable components, thus increasing their reliability for a wide range of applications.Thomas FolliardBarbara MertinsHarrison SteelThomas P. PrescottThomas NewportChristopher W. JonesGeorge WadhamsTravis BayerJudith P. ArmitageAntonis PapachristodoulouLynn J. RothschildNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Thomas Folliard Barbara Mertins Harrison Steel Thomas P. Prescott Thomas Newport Christopher W. Jones George Wadhams Travis Bayer Judith P. Armitage Antonis Papachristodoulou Lynn J. Rothschild Ribo-attenuators: novel elements for reliable and modular riboswitch engineering
description	Abstract Riboswitches are structural genetic regulatory elements that directly couple the sensing of small molecules to gene expression. They have considerable potential for applications throughout synthetic biology and bio-manufacturing as they are able to sense a wide range of small molecules and regulate gene expression in response. Despite over a decade of research they have yet to reach this considerable potential as they cannot yet be treated as modular components. This is due to several limitations including sensitivity to changes in genetic context, low tunability, and variability in performance. To overcome the associated difficulties with riboswitches, we have designed and introduced a novel genetic element called a ribo-attenuator in Bacteria. This genetic element allows for predictable tuning, insulation from contextual changes, and a reduction in expression variation. Ribo-attenuators allow riboswitches to be treated as truly modular and tunable components, thus increasing their reliability for a wide range of applications.
format	article
author	Thomas Folliard Barbara Mertins Harrison Steel Thomas P. Prescott Thomas Newport Christopher W. Jones George Wadhams Travis Bayer Judith P. Armitage Antonis Papachristodoulou Lynn J. Rothschild
author_facet	Thomas Folliard Barbara Mertins Harrison Steel Thomas P. Prescott Thomas Newport Christopher W. Jones George Wadhams Travis Bayer Judith P. Armitage Antonis Papachristodoulou Lynn J. Rothschild
author_sort	Thomas Folliard
title	Ribo-attenuators: novel elements for reliable and modular riboswitch engineering
title_short	Ribo-attenuators: novel elements for reliable and modular riboswitch engineering
title_full	Ribo-attenuators: novel elements for reliable and modular riboswitch engineering
title_fullStr	Ribo-attenuators: novel elements for reliable and modular riboswitch engineering
title_full_unstemmed	Ribo-attenuators: novel elements for reliable and modular riboswitch engineering
title_sort	ribo-attenuators: novel elements for reliable and modular riboswitch engineering
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/62d7c8bca79445f6a7fad7f630e03f74
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Ribo-attenuators: novel elements for reliable and modular riboswitch engineering

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