A modified formulation of Huanglian-Jie-Du-Tang reduces memory impairments and β-amyloid plaques in a triple transgenic mouse model of Alzheimer’s disease

Abstract Alzheimer’s disease (AD) is a degenerative disorder typified by progressive deterioration of memory and the appearance of β-amyloid peptide (Aβ)-rich senile plaques. Recently we have identified a novel function of a patented formulation of modified Huanglian-Jie-Tu-Tang (HLJDT-M), a Chinese...

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Autores principales:	Siva Sundara Kumar Durairajan, Ashok Iyaswamy, Sravan Gopalakrishna Shetty, Ananth Kumar Kammella, Sandeep Malampati, Wenbin Shang, Chuanbin Yang, Juxian Song, Sookja Chung, Jiandong Huang, Kaliappan Ilango, Quan-Bin Han, Min Li
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/62e10df4806746c0afad06ad28e8e202
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spelling	oai:doaj.org-article:62e10df4806746c0afad06ad28e8e2022021-12-02T11:53:04ZA modified formulation of Huanglian-Jie-Du-Tang reduces memory impairments and β-amyloid plaques in a triple transgenic mouse model of Alzheimer’s disease10.1038/s41598-017-06217-92045-2322https://doaj.org/article/62e10df4806746c0afad06ad28e8e2022017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-06217-9https://doaj.org/toc/2045-2322Abstract Alzheimer’s disease (AD) is a degenerative disorder typified by progressive deterioration of memory and the appearance of β-amyloid peptide (Aβ)-rich senile plaques. Recently we have identified a novel function of a patented formulation of modified Huanglian-Jie-Tu-Tang (HLJDT-M), a Chinese herbal medicine, in treating AD in in vitro studies (US patent No. 9,375,457). HLJDT-M is a formulation composed of Rhizoma Coptitis, Cortex Phellodendri and Fructus Gardeniae without Radix Scutellariae. Here, we assessed the efficacy of HLJDT-M on a triple transgenic mouse model of AD (3XTg-AD). Oral administration of HLJDT-M ameliorated the cognitive dysfunction of 3XTg-AD mice and lessened the plaque burden. In addition, biochemical assays revealed a significant decrease in levels of detergent-soluble and acid-soluble Aβ via decreasing the levels of full length amyloid-β precursor protein (FL-APP) and C-terminal fragments of APP (CTFs) in brain lysates of HLJDT-M-treated mice. HLJDT-M treatment also significantly reduced the levels of FL-APP and CTFs in N2a/SweAPP cells. In contrast, treatment using the classical formula HLJDT did not reduce the memory impairment of 3XTg-AD mice and, rather, increased the Aβ/Fl-APP/CTFs in both animal and cell culture studies. Altogether, our study indicates that HLJDT-M is a promising herbal formulation to prevent and/or cure AD.Siva Sundara Kumar DurairajanAshok IyaswamySravan Gopalakrishna ShettyAnanth Kumar KammellaSandeep MalampatiWenbin ShangChuanbin YangJuxian SongSookja ChungJiandong HuangKaliappan IlangoQuan-Bin HanMin LiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-13 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Siva Sundara Kumar Durairajan Ashok Iyaswamy Sravan Gopalakrishna Shetty Ananth Kumar Kammella Sandeep Malampati Wenbin Shang Chuanbin Yang Juxian Song Sookja Chung Jiandong Huang Kaliappan Ilango Quan-Bin Han Min Li A modified formulation of Huanglian-Jie-Du-Tang reduces memory impairments and β-amyloid plaques in a triple transgenic mouse model of Alzheimer’s disease
description	Abstract Alzheimer’s disease (AD) is a degenerative disorder typified by progressive deterioration of memory and the appearance of β-amyloid peptide (Aβ)-rich senile plaques. Recently we have identified a novel function of a patented formulation of modified Huanglian-Jie-Tu-Tang (HLJDT-M), a Chinese herbal medicine, in treating AD in in vitro studies (US patent No. 9,375,457). HLJDT-M is a formulation composed of Rhizoma Coptitis, Cortex Phellodendri and Fructus Gardeniae without Radix Scutellariae. Here, we assessed the efficacy of HLJDT-M on a triple transgenic mouse model of AD (3XTg-AD). Oral administration of HLJDT-M ameliorated the cognitive dysfunction of 3XTg-AD mice and lessened the plaque burden. In addition, biochemical assays revealed a significant decrease in levels of detergent-soluble and acid-soluble Aβ via decreasing the levels of full length amyloid-β precursor protein (FL-APP) and C-terminal fragments of APP (CTFs) in brain lysates of HLJDT-M-treated mice. HLJDT-M treatment also significantly reduced the levels of FL-APP and CTFs in N2a/SweAPP cells. In contrast, treatment using the classical formula HLJDT did not reduce the memory impairment of 3XTg-AD mice and, rather, increased the Aβ/Fl-APP/CTFs in both animal and cell culture studies. Altogether, our study indicates that HLJDT-M is a promising herbal formulation to prevent and/or cure AD.
format	article
author	Siva Sundara Kumar Durairajan Ashok Iyaswamy Sravan Gopalakrishna Shetty Ananth Kumar Kammella Sandeep Malampati Wenbin Shang Chuanbin Yang Juxian Song Sookja Chung Jiandong Huang Kaliappan Ilango Quan-Bin Han Min Li
author_facet	Siva Sundara Kumar Durairajan Ashok Iyaswamy Sravan Gopalakrishna Shetty Ananth Kumar Kammella Sandeep Malampati Wenbin Shang Chuanbin Yang Juxian Song Sookja Chung Jiandong Huang Kaliappan Ilango Quan-Bin Han Min Li
author_sort	Siva Sundara Kumar Durairajan
title	A modified formulation of Huanglian-Jie-Du-Tang reduces memory impairments and β-amyloid plaques in a triple transgenic mouse model of Alzheimer’s disease
title_short	A modified formulation of Huanglian-Jie-Du-Tang reduces memory impairments and β-amyloid plaques in a triple transgenic mouse model of Alzheimer’s disease
title_full	A modified formulation of Huanglian-Jie-Du-Tang reduces memory impairments and β-amyloid plaques in a triple transgenic mouse model of Alzheimer’s disease
title_fullStr	A modified formulation of Huanglian-Jie-Du-Tang reduces memory impairments and β-amyloid plaques in a triple transgenic mouse model of Alzheimer’s disease
title_full_unstemmed	A modified formulation of Huanglian-Jie-Du-Tang reduces memory impairments and β-amyloid plaques in a triple transgenic mouse model of Alzheimer’s disease
title_sort	modified formulation of huanglian-jie-du-tang reduces memory impairments and β-amyloid plaques in a triple transgenic mouse model of alzheimer’s disease
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/62e10df4806746c0afad06ad28e8e202
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A modified formulation of Huanglian-Jie-Du-Tang reduces memory impairments and β-amyloid plaques in a triple transgenic mouse model of Alzheimer’s disease

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