Expression and clinical significance of miRNA-146a and IRF-3 in children with infectious mononucleosis
Objective: To investigate the expression levels of interferon regulatory factor 3 (IRF-3) and miRNA146a in peripheral blood of children with infectious mononucleosis and the effect of miRNA146a on IRF-3 expression. Methods: In an in vivo experiment, the expression levels of IRF-3 and miRNA146a in...
Guardado en:
| Autores principales: | , |
|---|---|
| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
Editorial Board of Journal of Hainan Medical University
2021
|
| Materias: | |
| Acceso en línea: | https://doaj.org/article/62e8f300432d4fb38e01f5c18939c709 |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| Sumario: | Objective: To investigate the expression levels of interferon regulatory factor 3 (IRF-3) and
miRNA146a in peripheral blood of children with infectious mononucleosis and the effect of
miRNA146a on IRF-3 expression. Methods: In an in vivo experiment, the expression levels of
IRF-3 and miRNA146a in peripheral blood of 45 children with infectious mononucleosis and
34 healthy controls were detected by real-time PCR. The in vitro experiment was performed
to examine the effect of miRNA146a on IRF-3 expression levels by transfecting miRNA146a
mimics and their inhibitors. Results: There was significant difference in IRF-3 gene expression
in peripheral blood between healthy controls and children with infectious mononucleosis (t
= 30.340, P < 0.001) while miRNA146a expression was significantly increased compared
with healthy controls (t = 34.659, P < 0.001), and there was a negative correlation between
the two groups (r = -0.960, P < 0.05). In HeLa cells, transfection with miRNA146a mimics
significantly decreased the expression of IRF-3 mRNA (t = 8.270, P < 0.001) and protein (t
= 46.170, P < 0.001), while miRNA146a inhibitor significantly up-regulated the expression
of IRF-3 mRNA (t = 8.582, P < 0.001) and protein (t = 25.891, P < 0.001). Conclusion:
miRNA146a may be involved in the down-regulation of IRF-3 gene expression by Epstein-
Barr virus in children with infectious mononucleosis and then involved in the pathogenesis of
infectious mononucleosis. |
|---|