Expression and clinical significance of miRNA-146a and IRF-3 in children with infectious mononucleosis

Objective: To investigate the expression levels of interferon regulatory factor 3 (IRF-3) and miRNA146a in peripheral blood of children with infectious mononucleosis and the effect of miRNA146a on IRF-3 expression. Methods: In an in vivo experiment, the expression levels of IRF-3 and miRNA146a in...

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Autores principales:	Liang-Hua Zhu, Rui Jin
Formato:	article
Lenguaje:	EN
Publicado:	Editorial Board of Journal of Hainan Medical University 2021
Materias:	infectious mononucleosis syndrome mirna-146a interferon regulatory factor 3 Medicine R
Acceso en línea:	https://doaj.org/article/62e8f300432d4fb38e01f5c18939c709
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spelling	oai:doaj.org-article:62e8f300432d4fb38e01f5c18939c7092021-12-01T05:09:47ZExpression and clinical significance of miRNA-146a and IRF-3 in children with infectious mononucleosis1007-1237https://doaj.org/article/62e8f300432d4fb38e01f5c18939c7092021-10-01T00:00:00Zhttp://www.hnykdxxb.com/PDF/202120/04.pdfhttps://doaj.org/toc/1007-1237Objective: To investigate the expression levels of interferon regulatory factor 3 (IRF-3) and miRNA146a in peripheral blood of children with infectious mononucleosis and the effect of miRNA146a on IRF-3 expression. Methods: In an in vivo experiment, the expression levels of IRF-3 and miRNA146a in peripheral blood of 45 children with infectious mononucleosis and 34 healthy controls were detected by real-time PCR. The in vitro experiment was performed to examine the effect of miRNA146a on IRF-3 expression levels by transfecting miRNA146a mimics and their inhibitors. Results: There was significant difference in IRF-3 gene expression in peripheral blood between healthy controls and children with infectious mononucleosis (t = 30.340, P < 0.001) while miRNA146a expression was significantly increased compared with healthy controls (t = 34.659, P < 0.001), and there was a negative correlation between the two groups (r = -0.960, P < 0.05). In HeLa cells, transfection with miRNA146a mimics significantly decreased the expression of IRF-3 mRNA (t = 8.270, P < 0.001) and protein (t = 46.170, P < 0.001), while miRNA146a inhibitor significantly up-regulated the expression of IRF-3 mRNA (t = 8.582, P < 0.001) and protein (t = 25.891, P < 0.001). Conclusion: miRNA146a may be involved in the down-regulation of IRF-3 gene expression by Epstein- Barr virus in children with infectious mononucleosis and then involved in the pathogenesis of infectious mononucleosis.Liang-Hua ZhuRui JinEditorial Board of Journal of Hainan Medical Universityarticleinfectious mononucleosis syndromemirna-146ainterferon regulatory factor 3MedicineRENJournal of Hainan Medical University, Vol 27, Iss 20, Pp 15-19 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	infectious mononucleosis syndrome mirna-146a interferon regulatory factor 3 Medicine R
spellingShingle	infectious mononucleosis syndrome mirna-146a interferon regulatory factor 3 Medicine R Liang-Hua Zhu Rui Jin Expression and clinical significance of miRNA-146a and IRF-3 in children with infectious mononucleosis
description	Objective: To investigate the expression levels of interferon regulatory factor 3 (IRF-3) and miRNA146a in peripheral blood of children with infectious mononucleosis and the effect of miRNA146a on IRF-3 expression. Methods: In an in vivo experiment, the expression levels of IRF-3 and miRNA146a in peripheral blood of 45 children with infectious mononucleosis and 34 healthy controls were detected by real-time PCR. The in vitro experiment was performed to examine the effect of miRNA146a on IRF-3 expression levels by transfecting miRNA146a mimics and their inhibitors. Results: There was significant difference in IRF-3 gene expression in peripheral blood between healthy controls and children with infectious mononucleosis (t = 30.340, P < 0.001) while miRNA146a expression was significantly increased compared with healthy controls (t = 34.659, P < 0.001), and there was a negative correlation between the two groups (r = -0.960, P < 0.05). In HeLa cells, transfection with miRNA146a mimics significantly decreased the expression of IRF-3 mRNA (t = 8.270, P < 0.001) and protein (t = 46.170, P < 0.001), while miRNA146a inhibitor significantly up-regulated the expression of IRF-3 mRNA (t = 8.582, P < 0.001) and protein (t = 25.891, P < 0.001). Conclusion: miRNA146a may be involved in the down-regulation of IRF-3 gene expression by Epstein- Barr virus in children with infectious mononucleosis and then involved in the pathogenesis of infectious mononucleosis.
format	article
author	Liang-Hua Zhu Rui Jin
author_facet	Liang-Hua Zhu Rui Jin
author_sort	Liang-Hua Zhu
title	Expression and clinical significance of miRNA-146a and IRF-3 in children with infectious mononucleosis
title_short	Expression and clinical significance of miRNA-146a and IRF-3 in children with infectious mononucleosis
title_full	Expression and clinical significance of miRNA-146a and IRF-3 in children with infectious mononucleosis
title_fullStr	Expression and clinical significance of miRNA-146a and IRF-3 in children with infectious mononucleosis
title_full_unstemmed	Expression and clinical significance of miRNA-146a and IRF-3 in children with infectious mononucleosis
title_sort	expression and clinical significance of mirna-146a and irf-3 in children with infectious mononucleosis
publisher	Editorial Board of Journal of Hainan Medical University
publishDate	2021
url	https://doaj.org/article/62e8f300432d4fb38e01f5c18939c709
work_keys_str_mv	AT lianghuazhu expressionandclinicalsignificanceofmirna146aandirf3inchildrenwithinfectiousmononucleosis AT ruijin expressionandclinicalsignificanceofmirna146aandirf3inchildrenwithinfectiousmononucleosis
_version_	1718405544129069056

Expression and clinical significance of miRNA-146a and IRF-3 in children with infectious mononucleosis

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