Role of surface charge in determining the biological effects of CdSe/ZnS quantum dots

Qiangqiang Liu,1,* Hongxia Li,1,* Qiyue Xia,1 Ying Liu,1 Kai Xiao,1,2 1National Chengdu Center for Safety Evaluation of Drugs, State Key Laboratory of Biotherapy, Collaborative Innovation Center for Biotherapy, West China Hospital, 2Laboratory of Non-Human Primate Disease Model Research, State Key...

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Autores principales:	Liu QQ, Li HX, Xia QY, Liu Y, Xiao K
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2015
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/62ebf89efcbe48fead78daa1b25ef147
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spelling	oai:doaj.org-article:62ebf89efcbe48fead78daa1b25ef1472021-12-02T02:10:35ZRole of surface charge in determining the biological effects of CdSe/ZnS quantum dots1178-2013https://doaj.org/article/62ebf89efcbe48fead78daa1b25ef1472015-11-01T00:00:00Zhttps://www.dovepress.com/role-of-surface-charge-in-determining-the-biological-effects-of-cdsezn-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Qiangqiang Liu,1,* Hongxia Li,1,* Qiyue Xia,1 Ying Liu,1 Kai Xiao,1,2 1National Chengdu Center for Safety Evaluation of Drugs, State Key Laboratory of Biotherapy, Collaborative Innovation Center for Biotherapy, West China Hospital, 2Laboratory of Non-Human Primate Disease Model Research, State Key Laboratory of Biotherapy, Collaborative Innovation Center for Biotherapy, West China Hospital, Sichuan University, Chengdu, People’s Republic of China *These authors contributed equally to this work Abstract: The growing potential of quantum dots (QDs) in biomedical applications has provoked the urgent need to thoroughly address their interaction with biological systems. However, only limited studies have been performed to explore the effects of surface charge on the biological behaviors of QDs. In the present study, three commercially available QDs with different surface coatings were used to systematically evaluate the effects of surface charge on the cellular uptake, cytotoxicity, and in vivo biodistribution of QDs. Our results demonstrated that charged QDs entered both cancer cells and macrophages more efficiently than neutral ones, while negative QDs internalized mostly. Upon entry into cells, QDs were localized in different subcellular compartments (eg, cytoplasm and lysosomes) depending on the surface charge. Interestingly, inconsistent with the result of internalization, positive QDs but not negative QDs exhibited severe cytotoxicity, which was likely due to their disruption of cell membrane integrity, and production of reactive oxygen species. Biodistribution studies demonstrated that negative and neutral QDs preferentially distributed in the liver and the spleen, whereas positive QDs mainly deposited in the kidney with obvious uptake in the brain. In general, surface charge plays crucial roles in determining the biological interactions of QDs. Keywords: cellular uptake, uptake pathways, intracellular distribution, reactive oxygen species, cytotoxicity, biodistributionLiu QQLi HXXia QYLiu YXiao KDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2015, Iss default, Pp 7073-7088 (2015)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Liu QQ Li HX Xia QY Liu Y Xiao K Role of surface charge in determining the biological effects of CdSe/ZnS quantum dots
description	Qiangqiang Liu,1,* Hongxia Li,1,* Qiyue Xia,1 Ying Liu,1 Kai Xiao,1,2 1National Chengdu Center for Safety Evaluation of Drugs, State Key Laboratory of Biotherapy, Collaborative Innovation Center for Biotherapy, West China Hospital, 2Laboratory of Non-Human Primate Disease Model Research, State Key Laboratory of Biotherapy, Collaborative Innovation Center for Biotherapy, West China Hospital, Sichuan University, Chengdu, People’s Republic of China *These authors contributed equally to this work Abstract: The growing potential of quantum dots (QDs) in biomedical applications has provoked the urgent need to thoroughly address their interaction with biological systems. However, only limited studies have been performed to explore the effects of surface charge on the biological behaviors of QDs. In the present study, three commercially available QDs with different surface coatings were used to systematically evaluate the effects of surface charge on the cellular uptake, cytotoxicity, and in vivo biodistribution of QDs. Our results demonstrated that charged QDs entered both cancer cells and macrophages more efficiently than neutral ones, while negative QDs internalized mostly. Upon entry into cells, QDs were localized in different subcellular compartments (eg, cytoplasm and lysosomes) depending on the surface charge. Interestingly, inconsistent with the result of internalization, positive QDs but not negative QDs exhibited severe cytotoxicity, which was likely due to their disruption of cell membrane integrity, and production of reactive oxygen species. Biodistribution studies demonstrated that negative and neutral QDs preferentially distributed in the liver and the spleen, whereas positive QDs mainly deposited in the kidney with obvious uptake in the brain. In general, surface charge plays crucial roles in determining the biological interactions of QDs. Keywords: cellular uptake, uptake pathways, intracellular distribution, reactive oxygen species, cytotoxicity, biodistribution
format	article
author	Liu QQ Li HX Xia QY Liu Y Xiao K
author_facet	Liu QQ Li HX Xia QY Liu Y Xiao K
author_sort	Liu QQ
title	Role of surface charge in determining the biological effects of CdSe/ZnS quantum dots
title_short	Role of surface charge in determining the biological effects of CdSe/ZnS quantum dots
title_full	Role of surface charge in determining the biological effects of CdSe/ZnS quantum dots
title_fullStr	Role of surface charge in determining the biological effects of CdSe/ZnS quantum dots
title_full_unstemmed	Role of surface charge in determining the biological effects of CdSe/ZnS quantum dots
title_sort	role of surface charge in determining the biological effects of cdse/zns quantum dots
publisher	Dove Medical Press
publishDate	2015
url	https://doaj.org/article/62ebf89efcbe48fead78daa1b25ef147
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Role of surface charge in determining the biological effects of CdSe/ZnS quantum dots

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