Electrochemical sensing method for point-of-care cortisol detection in human immunodeficiency virus-infected patients

Ajeet Kaushik,1 Adriana Yndart,1 Rahul Dev Jayant,1 Vidya Sagar,1 Venkata Atluri,1 Shekhar Bhansali,2 Madhavan Nair11Center of Personalized Nanomedicine, Institute of Neuroimmune Pharmacology, Department of Immunology, Herbert Wertheim College of Medicine, Florida International University,...

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Autores principales:	Kaushik A, Yndart A, Jayant RD, Sagar V, Atluri V, Bhansali S, Nair M
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2015
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/62f1e602e10b46ecb9c62f81ba2c0445
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spelling	oai:doaj.org-article:62f1e602e10b46ecb9c62f81ba2c04452021-12-02T03:55:32ZElectrochemical sensing method for point-of-care cortisol detection in human immunodeficiency virus-infected patients1178-2013https://doaj.org/article/62f1e602e10b46ecb9c62f81ba2c04452015-01-01T00:00:00Zhttp://www.dovepress.com/electrochemical-sensing-method-for-point-of-care-cortisol-detection-in-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013 Ajeet Kaushik,1 Adriana Yndart,1 Rahul Dev Jayant,1 Vidya Sagar,1 Venkata Atluri,1 Shekhar Bhansali,2 Madhavan Nair11Center of Personalized Nanomedicine, Institute of Neuroimmune Pharmacology, Department of Immunology, Herbert Wertheim College of Medicine, Florida International University, 2BioMEMS Microsystems Laboratory, Department of Electrical and Computer Engineering, Florida International University, Miami, FL, USA Abstract: A novel electrochemical sensing method was devised for the first time to detect plasma cortisol, a potential psychological stress biomarker, in human immunodeficiency virus (HIV)-positive subjects. A miniaturized potentiostat (reconfigured LMP91000 chip) interfaced with a microfluidic manifold containing a cortisol immunosensor was employed to demonstrate electrochemical cortisol sensing. This fully integrated and optimized electrochemical sensing device exhibited a wide cortisol-detection range from 10 pg/mL to 500 ng/mL, a low detection limit of 10 pg/mL, and sensitivity of 5.8 µA (pg mL)-1, with a regression coefficient of 0.995. This cortisol-selective sensing system was employed to estimate plasma cortisol in ten samples from HIV patients. The electrochemical cortisol-sensing performance was validated using an enzyme-linked immunosorbent assay technique. The results obtained using both methodologies were comparable within 2%–5% variation. The information related to psychological stress of HIV patients can be correlated with disease-progression parameters to optimize diagnosis, therapeutic, and personalized health monitoring.Keywords: psychological stress, personalized health care, cortisol, HIV, electrochemical immunosensing, miniaturized sensing deviceKaushik AYndart AJayant RDSagar VAtluri VBhansali SNair MDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2015, Iss default, Pp 677-685 (2015)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Kaushik A Yndart A Jayant RD Sagar V Atluri V Bhansali S Nair M Electrochemical sensing method for point-of-care cortisol detection in human immunodeficiency virus-infected patients
description	Ajeet Kaushik,1 Adriana Yndart,1 Rahul Dev Jayant,1 Vidya Sagar,1 Venkata Atluri,1 Shekhar Bhansali,2 Madhavan Nair11Center of Personalized Nanomedicine, Institute of Neuroimmune Pharmacology, Department of Immunology, Herbert Wertheim College of Medicine, Florida International University, 2BioMEMS Microsystems Laboratory, Department of Electrical and Computer Engineering, Florida International University, Miami, FL, USA Abstract: A novel electrochemical sensing method was devised for the first time to detect plasma cortisol, a potential psychological stress biomarker, in human immunodeficiency virus (HIV)-positive subjects. A miniaturized potentiostat (reconfigured LMP91000 chip) interfaced with a microfluidic manifold containing a cortisol immunosensor was employed to demonstrate electrochemical cortisol sensing. This fully integrated and optimized electrochemical sensing device exhibited a wide cortisol-detection range from 10 pg/mL to 500 ng/mL, a low detection limit of 10 pg/mL, and sensitivity of 5.8 µA (pg mL)-1, with a regression coefficient of 0.995. This cortisol-selective sensing system was employed to estimate plasma cortisol in ten samples from HIV patients. The electrochemical cortisol-sensing performance was validated using an enzyme-linked immunosorbent assay technique. The results obtained using both methodologies were comparable within 2%–5% variation. The information related to psychological stress of HIV patients can be correlated with disease-progression parameters to optimize diagnosis, therapeutic, and personalized health monitoring.Keywords: psychological stress, personalized health care, cortisol, HIV, electrochemical immunosensing, miniaturized sensing device
format	article
author	Kaushik A Yndart A Jayant RD Sagar V Atluri V Bhansali S Nair M
author_facet	Kaushik A Yndart A Jayant RD Sagar V Atluri V Bhansali S Nair M
author_sort	Kaushik A
title	Electrochemical sensing method for point-of-care cortisol detection in human immunodeficiency virus-infected patients
title_short	Electrochemical sensing method for point-of-care cortisol detection in human immunodeficiency virus-infected patients
title_full	Electrochemical sensing method for point-of-care cortisol detection in human immunodeficiency virus-infected patients
title_fullStr	Electrochemical sensing method for point-of-care cortisol detection in human immunodeficiency virus-infected patients
title_full_unstemmed	Electrochemical sensing method for point-of-care cortisol detection in human immunodeficiency virus-infected patients
title_sort	electrochemical sensing method for point-of-care cortisol detection in human immunodeficiency virus-infected patients
publisher	Dove Medical Press
publishDate	2015
url	https://doaj.org/article/62f1e602e10b46ecb9c62f81ba2c0445
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Electrochemical sensing method for point-of-care cortisol detection in human immunodeficiency virus-infected patients

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