Battle of the Bacteria: Characterizing the Evolutionary Advantage of Stationary Phase Growth

Providing students with authentic research opportunities has been shown to enhance learning and increase retention in STEM majors. Accordingly, we have developed a novel microbiology lab module, which focuses on the molecular mechanisms of evolution in E. coli, by examining the growth advantage in s...

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Autores principales:	Karin E. Kram, Kristina M. Yim, Aaron B. Coleman, Brian K. Sato
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2016
Materias:	Special aspects of education LC8-6691 Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/62f6a08732b84757a7bc528de31dd83d
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spelling	oai:doaj.org-article:62f6a08732b84757a7bc528de31dd83d2021-11-15T15:03:46ZBattle of the Bacteria: Characterizing the Evolutionary Advantage of Stationary Phase Growth10.1128/jmbe.v17i2.9811935-78851935-7877https://doaj.org/article/62f6a08732b84757a7bc528de31dd83d2016-05-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/jmbe.v17i2.981https://doaj.org/toc/1935-7877https://doaj.org/toc/1935-7885Providing students with authentic research opportunities has been shown to enhance learning and increase retention in STEM majors. Accordingly, we have developed a novel microbiology lab module, which focuses on the molecular mechanisms of evolution in E. coli, by examining the growth advantage in stationary phase (GASP) phenotype. The GASP phenotype is demonstrated by growing cells into long-term stationary phase (LTSP) and then competing them against un-aged cells in a fresh culture. This module includes learning goals related to strengthening practical laboratory skills and improving student understanding of evolution. In addition, the students generate novel data regarding the effects of different environmental stresses on GASP and the relationship between evolution, genotypic change, mutation frequency, and cell stress. Pairs of students are provided with the experimental background, select a specific aspect of the growth medium to modify, and generate a hypothesis regarding how this alteration will impact the GASP phenotype. From this module, we have demonstrated that students are able to achieve the established learning goals and have produced data that has furthered our understanding of the GASP phenotype. Journal of Microbiology & Biology EducationKarin E. KramKristina M. YimAaron B. ColemanBrian K. SatoAmerican Society for MicrobiologyarticleSpecial aspects of educationLC8-6691Biology (General)QH301-705.5ENJournal of Microbiology & Biology Education, Vol 17, Iss 2, Pp 261-268 (2016)
institution	DOAJ
collection	DOAJ
language	EN
topic	Special aspects of education LC8-6691 Biology (General) QH301-705.5
spellingShingle	Special aspects of education LC8-6691 Biology (General) QH301-705.5 Karin E. Kram Kristina M. Yim Aaron B. Coleman Brian K. Sato Battle of the Bacteria: Characterizing the Evolutionary Advantage of Stationary Phase Growth
description	Providing students with authentic research opportunities has been shown to enhance learning and increase retention in STEM majors. Accordingly, we have developed a novel microbiology lab module, which focuses on the molecular mechanisms of evolution in E. coli, by examining the growth advantage in stationary phase (GASP) phenotype. The GASP phenotype is demonstrated by growing cells into long-term stationary phase (LTSP) and then competing them against un-aged cells in a fresh culture. This module includes learning goals related to strengthening practical laboratory skills and improving student understanding of evolution. In addition, the students generate novel data regarding the effects of different environmental stresses on GASP and the relationship between evolution, genotypic change, mutation frequency, and cell stress. Pairs of students are provided with the experimental background, select a specific aspect of the growth medium to modify, and generate a hypothesis regarding how this alteration will impact the GASP phenotype. From this module, we have demonstrated that students are able to achieve the established learning goals and have produced data that has furthered our understanding of the GASP phenotype. Journal of Microbiology & Biology Education
format	article
author	Karin E. Kram Kristina M. Yim Aaron B. Coleman Brian K. Sato
author_facet	Karin E. Kram Kristina M. Yim Aaron B. Coleman Brian K. Sato
author_sort	Karin E. Kram
title	Battle of the Bacteria: Characterizing the Evolutionary Advantage of Stationary Phase Growth
title_short	Battle of the Bacteria: Characterizing the Evolutionary Advantage of Stationary Phase Growth
title_full	Battle of the Bacteria: Characterizing the Evolutionary Advantage of Stationary Phase Growth
title_fullStr	Battle of the Bacteria: Characterizing the Evolutionary Advantage of Stationary Phase Growth
title_full_unstemmed	Battle of the Bacteria: Characterizing the Evolutionary Advantage of Stationary Phase Growth
title_sort	battle of the bacteria: characterizing the evolutionary advantage of stationary phase growth
publisher	American Society for Microbiology
publishDate	2016
url	https://doaj.org/article/62f6a08732b84757a7bc528de31dd83d
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Battle of the Bacteria: Characterizing the Evolutionary Advantage of Stationary Phase Growth

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