Detection of Plasmodium falciparum in laboratory-reared and naturally infected wild mosquitoes using near-infrared spectroscopy

Abstract There is an urgent need for high throughput, affordable methods of detecting pathogens inside insect vectors to facilitate surveillance. Near-infrared spectroscopy (NIRS) has shown promise to detect arbovirus and malaria in the laboratory but has not been evaluated in field conditions. Here...

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Autores principales:	Dari F. Da, Ruth McCabe, Bernard M. Somé, Pedro M. Esperança, Katarzyna A. Sala, Josua Blight, Andrew M. Blagborough, Floyd Dowell, Serge R. Yerbanga, Thierry Lefèvre, Karine Mouline, Roch K. Dabiré, Thomas S. Churcher
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/62f6f2a286bc46f793a6a8c2cea546b1
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spelling	oai:doaj.org-article:62f6f2a286bc46f793a6a8c2cea546b12021-12-02T15:55:13ZDetection of Plasmodium falciparum in laboratory-reared and naturally infected wild mosquitoes using near-infrared spectroscopy10.1038/s41598-021-89715-12045-2322https://doaj.org/article/62f6f2a286bc46f793a6a8c2cea546b12021-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-89715-1https://doaj.org/toc/2045-2322Abstract There is an urgent need for high throughput, affordable methods of detecting pathogens inside insect vectors to facilitate surveillance. Near-infrared spectroscopy (NIRS) has shown promise to detect arbovirus and malaria in the laboratory but has not been evaluated in field conditions. Here we investigate the ability of NIRS to identify Plasmodium falciparum in Anopheles coluzzii mosquitoes. NIRS models trained on laboratory-reared mosquitoes infected with wild malaria parasites can detect the parasite in comparable mosquitoes with moderate accuracy though fails to detect oocysts or sporozoites in naturally infected field caught mosquitoes. Models trained on field mosquitoes were unable to predict the infection status of other field mosquitoes. Restricting analyses to mosquitoes of uninfectious and highly-infectious status did improve predictions suggesting sensitivity and specificity may be better in mosquitoes with higher numbers of parasites. Detection of infection appears restricted to homogenous groups of mosquitoes diminishing NIRS utility for detecting malaria within mosquitoes.Dari F. DaRuth McCabeBernard M. SoméPedro M. EsperançaKatarzyna A. SalaJosua BlightAndrew M. BlagboroughFloyd DowellSerge R. YerbangaThierry LefèvreKarine MoulineRoch K. DabiréThomas S. ChurcherNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-8 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Dari F. Da Ruth McCabe Bernard M. Somé Pedro M. Esperança Katarzyna A. Sala Josua Blight Andrew M. Blagborough Floyd Dowell Serge R. Yerbanga Thierry Lefèvre Karine Mouline Roch K. Dabiré Thomas S. Churcher Detection of Plasmodium falciparum in laboratory-reared and naturally infected wild mosquitoes using near-infrared spectroscopy
description	Abstract There is an urgent need for high throughput, affordable methods of detecting pathogens inside insect vectors to facilitate surveillance. Near-infrared spectroscopy (NIRS) has shown promise to detect arbovirus and malaria in the laboratory but has not been evaluated in field conditions. Here we investigate the ability of NIRS to identify Plasmodium falciparum in Anopheles coluzzii mosquitoes. NIRS models trained on laboratory-reared mosquitoes infected with wild malaria parasites can detect the parasite in comparable mosquitoes with moderate accuracy though fails to detect oocysts or sporozoites in naturally infected field caught mosquitoes. Models trained on field mosquitoes were unable to predict the infection status of other field mosquitoes. Restricting analyses to mosquitoes of uninfectious and highly-infectious status did improve predictions suggesting sensitivity and specificity may be better in mosquitoes with higher numbers of parasites. Detection of infection appears restricted to homogenous groups of mosquitoes diminishing NIRS utility for detecting malaria within mosquitoes.
format	article
author	Dari F. Da Ruth McCabe Bernard M. Somé Pedro M. Esperança Katarzyna A. Sala Josua Blight Andrew M. Blagborough Floyd Dowell Serge R. Yerbanga Thierry Lefèvre Karine Mouline Roch K. Dabiré Thomas S. Churcher
author_facet	Dari F. Da Ruth McCabe Bernard M. Somé Pedro M. Esperança Katarzyna A. Sala Josua Blight Andrew M. Blagborough Floyd Dowell Serge R. Yerbanga Thierry Lefèvre Karine Mouline Roch K. Dabiré Thomas S. Churcher
author_sort	Dari F. Da
title	Detection of Plasmodium falciparum in laboratory-reared and naturally infected wild mosquitoes using near-infrared spectroscopy
title_short	Detection of Plasmodium falciparum in laboratory-reared and naturally infected wild mosquitoes using near-infrared spectroscopy
title_full	Detection of Plasmodium falciparum in laboratory-reared and naturally infected wild mosquitoes using near-infrared spectroscopy
title_fullStr	Detection of Plasmodium falciparum in laboratory-reared and naturally infected wild mosquitoes using near-infrared spectroscopy
title_full_unstemmed	Detection of Plasmodium falciparum in laboratory-reared and naturally infected wild mosquitoes using near-infrared spectroscopy
title_sort	detection of plasmodium falciparum in laboratory-reared and naturally infected wild mosquitoes using near-infrared spectroscopy
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/62f6f2a286bc46f793a6a8c2cea546b1
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Detection of Plasmodium falciparum in laboratory-reared and naturally infected wild mosquitoes using near-infrared spectroscopy

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