Dual Function of Silver Nanoparticles as Matrix Extracell Removal and Antimicrobial Agent in Polymycrobial Biofilms

Candida albicans often form polymicrobial biofilms along with pathogenic microbes. Silver nanoparticles (AgNPs) were well known to have strong antimicrobial activity. However, their effect on polymicrobial biofilms and the mechanism has never been reported. This study aimed to synthesize AgNPs and s...

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Autores principales:	Mei Shirli Yasinta, Hera Lisna Ginawati, Nira Ambar Arum, Harini Nur Hikmah, Sri Sumarsih, Mochamad Zakki Fahmi, Afaf Baktir
Formato:	article
Lenguaje:	EN
Publicado:	Department of Chemistry, Universitas Gadjah Mada 2021
Materias:	silver nanoparticle polymicrobial biofilm c. albicans e. coli anti-biofilm Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/62f9432c5cf94fdc84c7d592a5da1c9a
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spelling	oai:doaj.org-article:62f9432c5cf94fdc84c7d592a5da1c9a2021-12-02T14:45:03ZDual Function of Silver Nanoparticles as Matrix Extracell Removal and Antimicrobial Agent in Polymycrobial Biofilms1411-94202460-157810.22146/ijc.52355https://doaj.org/article/62f9432c5cf94fdc84c7d592a5da1c9a2021-03-01T00:00:00Zhttps://jurnal.ugm.ac.id/ijc/article/view/52355https://doaj.org/toc/1411-9420https://doaj.org/toc/2460-1578Candida albicans often form polymicrobial biofilms along with pathogenic microbes. Silver nanoparticles (AgNPs) were well known to have strong antimicrobial activity. However, their effect on polymicrobial biofilms and the mechanism has never been reported. This study aimed to synthesize AgNPs and study their effects on polymicrobial biofilm represented by C. albicans–E. coli biofilm. Polymicrobial biofilms, formed by clinical isolates of C. albicans and E. coli, were developed from the standardized suspensions of each strain by culturing flat-bottom 96-well microtiter plates for 48 h, then treated with AgNPs. Cell viability was assessed using the tetrazolium salt reduction assay; the extent of biofilm formation was measured by crystal violet staining. AgNPs reduced the polymicrobial biofilm in two ways: by degrading the extracellular matrix and killing both C. albicans and E. coli. The results showed AgNPs is a potential new approach for developing potent anti-biofilms.Mei Shirli YasintaHera Lisna GinawatiNira Ambar ArumHarini Nur HikmahSri SumarsihMochamad Zakki FahmiAfaf BaktirDepartment of Chemistry, Universitas Gadjah Madaarticlesilver nanoparticlepolymicrobial biofilmc. albicanse. colianti-biofilmChemistryQD1-999ENIndonesian Journal of Chemistry, Vol 21, Iss 2, Pp 286-295 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	silver nanoparticle polymicrobial biofilm c. albicans e. coli anti-biofilm Chemistry QD1-999
spellingShingle	silver nanoparticle polymicrobial biofilm c. albicans e. coli anti-biofilm Chemistry QD1-999 Mei Shirli Yasinta Hera Lisna Ginawati Nira Ambar Arum Harini Nur Hikmah Sri Sumarsih Mochamad Zakki Fahmi Afaf Baktir Dual Function of Silver Nanoparticles as Matrix Extracell Removal and Antimicrobial Agent in Polymycrobial Biofilms
description	Candida albicans often form polymicrobial biofilms along with pathogenic microbes. Silver nanoparticles (AgNPs) were well known to have strong antimicrobial activity. However, their effect on polymicrobial biofilms and the mechanism has never been reported. This study aimed to synthesize AgNPs and study their effects on polymicrobial biofilm represented by C. albicans–E. coli biofilm. Polymicrobial biofilms, formed by clinical isolates of C. albicans and E. coli, were developed from the standardized suspensions of each strain by culturing flat-bottom 96-well microtiter plates for 48 h, then treated with AgNPs. Cell viability was assessed using the tetrazolium salt reduction assay; the extent of biofilm formation was measured by crystal violet staining. AgNPs reduced the polymicrobial biofilm in two ways: by degrading the extracellular matrix and killing both C. albicans and E. coli. The results showed AgNPs is a potential new approach for developing potent anti-biofilms.
format	article
author	Mei Shirli Yasinta Hera Lisna Ginawati Nira Ambar Arum Harini Nur Hikmah Sri Sumarsih Mochamad Zakki Fahmi Afaf Baktir
author_facet	Mei Shirli Yasinta Hera Lisna Ginawati Nira Ambar Arum Harini Nur Hikmah Sri Sumarsih Mochamad Zakki Fahmi Afaf Baktir
author_sort	Mei Shirli Yasinta
title	Dual Function of Silver Nanoparticles as Matrix Extracell Removal and Antimicrobial Agent in Polymycrobial Biofilms
title_short	Dual Function of Silver Nanoparticles as Matrix Extracell Removal and Antimicrobial Agent in Polymycrobial Biofilms
title_full	Dual Function of Silver Nanoparticles as Matrix Extracell Removal and Antimicrobial Agent in Polymycrobial Biofilms
title_fullStr	Dual Function of Silver Nanoparticles as Matrix Extracell Removal and Antimicrobial Agent in Polymycrobial Biofilms
title_full_unstemmed	Dual Function of Silver Nanoparticles as Matrix Extracell Removal and Antimicrobial Agent in Polymycrobial Biofilms
title_sort	dual function of silver nanoparticles as matrix extracell removal and antimicrobial agent in polymycrobial biofilms
publisher	Department of Chemistry, Universitas Gadjah Mada
publishDate	2021
url	https://doaj.org/article/62f9432c5cf94fdc84c7d592a5da1c9a
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Dual Function of Silver Nanoparticles as Matrix Extracell Removal and Antimicrobial Agent in Polymycrobial Biofilms

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