Antiviral activities of Schizonepeta tenuifolia Briq. against enterovirus 71 in vitro and in vivo

Abstract No effective drug is currently available for treatment of enterovirus 71 (EV71) infection. Schizonepeta tenuifolia Briq. (ST) has been used as a herbal constituent of traditional Chinese medicine. We studied whether the aqueous extract of Schizonepeta tenuifolia Briq (STE) has antiviral act...

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Autores principales:	Sin-Guang Chen, Mei-Ling Cheng, Kuan-Hsing Chen, Jim-Tong Horng, Ching-Chuan Liu, Shih-Min Wang, Hiroaki Sakurai, Yann-Lii Leu, Shulhn-Der Wang, Hung-Yao Ho
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/62fbda5601f3486ab8efb7dbc248c0e3
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spelling	oai:doaj.org-article:62fbda5601f3486ab8efb7dbc248c0e32021-12-02T11:53:14ZAntiviral activities of Schizonepeta tenuifolia Briq. against enterovirus 71 in vitro and in vivo10.1038/s41598-017-01110-x2045-2322https://doaj.org/article/62fbda5601f3486ab8efb7dbc248c0e32017-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-01110-xhttps://doaj.org/toc/2045-2322Abstract No effective drug is currently available for treatment of enterovirus 71 (EV71) infection. Schizonepeta tenuifolia Briq. (ST) has been used as a herbal constituent of traditional Chinese medicine. We studied whether the aqueous extract of Schizonepeta tenuifolia Briq (STE) has antiviral activity. STE inhibited replication of EV71, as evident by its ability to diminish plaque formation and cytopathic effect induced by EV71, and to inhibit the synthesis of viral RNA and protein. Moreover, daily single-dose STE treatment significantly improved the survival of EV71-infected mice, and ameliorated the symptoms. Mechanistically, STE exerts multiple effects on enteroviral infection. Treatment with STE reduced viral attachment and entry; the cleavage of eukaryotic translation initiation factor 4 G (eIF4G) by EV71 protease, 2Apro; virus-induced reactive oxygen species (ROS) formation; and relocation of heterogeneous nuclear ribonucleoprotein A1 (hnRNP A1) from the nucleus to the cytoplasm. It was accompanied by a decline in EV71-associated hyperphosphorylation of p38 kinase and EPS15. It is plausible that STE may inhibit ROS-induced p38 kinase activation, and subsequent hnRNP A1 relocation and EPS15-mediated membrane trafficking in infected cells. These findings suggest that STE possesses anti-EV71 activities, and may serve as health food or candidate antiviral drug for protection against EV71.Sin-Guang ChenMei-Ling ChengKuan-Hsing ChenJim-Tong HorngChing-Chuan LiuShih-Min WangHiroaki SakuraiYann-Lii LeuShulhn-Der WangHung-Yao HoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-15 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Sin-Guang Chen Mei-Ling Cheng Kuan-Hsing Chen Jim-Tong Horng Ching-Chuan Liu Shih-Min Wang Hiroaki Sakurai Yann-Lii Leu Shulhn-Der Wang Hung-Yao Ho Antiviral activities of Schizonepeta tenuifolia Briq. against enterovirus 71 in vitro and in vivo
description	Abstract No effective drug is currently available for treatment of enterovirus 71 (EV71) infection. Schizonepeta tenuifolia Briq. (ST) has been used as a herbal constituent of traditional Chinese medicine. We studied whether the aqueous extract of Schizonepeta tenuifolia Briq (STE) has antiviral activity. STE inhibited replication of EV71, as evident by its ability to diminish plaque formation and cytopathic effect induced by EV71, and to inhibit the synthesis of viral RNA and protein. Moreover, daily single-dose STE treatment significantly improved the survival of EV71-infected mice, and ameliorated the symptoms. Mechanistically, STE exerts multiple effects on enteroviral infection. Treatment with STE reduced viral attachment and entry; the cleavage of eukaryotic translation initiation factor 4 G (eIF4G) by EV71 protease, 2Apro; virus-induced reactive oxygen species (ROS) formation; and relocation of heterogeneous nuclear ribonucleoprotein A1 (hnRNP A1) from the nucleus to the cytoplasm. It was accompanied by a decline in EV71-associated hyperphosphorylation of p38 kinase and EPS15. It is plausible that STE may inhibit ROS-induced p38 kinase activation, and subsequent hnRNP A1 relocation and EPS15-mediated membrane trafficking in infected cells. These findings suggest that STE possesses anti-EV71 activities, and may serve as health food or candidate antiviral drug for protection against EV71.
format	article
author	Sin-Guang Chen Mei-Ling Cheng Kuan-Hsing Chen Jim-Tong Horng Ching-Chuan Liu Shih-Min Wang Hiroaki Sakurai Yann-Lii Leu Shulhn-Der Wang Hung-Yao Ho
author_facet	Sin-Guang Chen Mei-Ling Cheng Kuan-Hsing Chen Jim-Tong Horng Ching-Chuan Liu Shih-Min Wang Hiroaki Sakurai Yann-Lii Leu Shulhn-Der Wang Hung-Yao Ho
author_sort	Sin-Guang Chen
title	Antiviral activities of Schizonepeta tenuifolia Briq. against enterovirus 71 in vitro and in vivo
title_short	Antiviral activities of Schizonepeta tenuifolia Briq. against enterovirus 71 in vitro and in vivo
title_full	Antiviral activities of Schizonepeta tenuifolia Briq. against enterovirus 71 in vitro and in vivo
title_fullStr	Antiviral activities of Schizonepeta tenuifolia Briq. against enterovirus 71 in vitro and in vivo
title_full_unstemmed	Antiviral activities of Schizonepeta tenuifolia Briq. against enterovirus 71 in vitro and in vivo
title_sort	antiviral activities of schizonepeta tenuifolia briq. against enterovirus 71 in vitro and in vivo
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/62fbda5601f3486ab8efb7dbc248c0e3
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Antiviral activities of Schizonepeta tenuifolia Briq. against enterovirus 71 in vitro and in vivo

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