Identification of DELLA Genes and Key Stage for GA Sensitivity in Bolting and Flowering of Flowering Chinese Cabbage

Flowering Chinese cabbage (Brassica campestris L. ssp. chinensis var. utilis Tsen et Lee) is an important and extensively cultivated vegetable in south China, and its stalk development is mainly regulated by gibberellin (GA). DELLA proteins ne...

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Autores principales:	Hongling Guan, Xinmin Huang, Yunna Zhu, Baoxing Xie, Houcheng Liu, Shiwei Song, Yanwei Hao, Riyuan Chen
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	flowering Chinese cabbage DELLA gibberellin signaling bolting and flowering flower bud differentiation Biology (General) QH301-705.5 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/62fe863b7fb64d658aaff1a598799801
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spelling	oai:doaj.org-article:62fe863b7fb64d658aaff1a5987998012021-11-25T17:53:21ZIdentification of <i>DELLA</i> Genes and Key Stage for GA Sensitivity in Bolting and Flowering of Flowering Chinese Cabbage10.3390/ijms2222120921422-00671661-6596https://doaj.org/article/62fe863b7fb64d658aaff1a5987998012021-11-01T00:00:00Zhttps://www.mdpi.com/1422-0067/22/22/12092https://doaj.org/toc/1661-6596https://doaj.org/toc/1422-0067Flowering Chinese cabbage (<i>Brassica campestris</i> L. ssp. <i>chinensis</i> var. <i>utilis</i> Tsen et Lee) is an important and extensively cultivated vegetable in south China, and its stalk development is mainly regulated by gibberellin (GA). DELLA proteins negatively regulate GA signal transduction and may play an important role in determining bolting and flowering. Nevertheless, no systematic study of the <i>DELLA</i> gene family has been undertaken in flowering Chinese cabbage. In the present study, we found that the two-true-leaf spraying of gibberellin A3 (GA<sub>3</sub>) did not promote bolting but did promote flowering, whereas the three-true-leaf spraying of GA<sub>3</sub> promoted both bolting and flowering. In addition, we identified five <i>DELLA</i> genes in flowering Chinese cabbage. All five proteins contained DELLA, VHYNP, VHIID, and SAW conserved domains. Protein-protein interaction results showed that in the presence of GA<sub>3</sub>, all five DELLA proteins interacted with BcGID1b (GA-INSENSITIVE DWARF 1b) but not with BcGID1a (GA-INSENSITIVE DWARF 1a) or BcGID1c (GA-INSENSITIVE DWARF 1c). Their expression analysis showed that the <i>DELLA</i> genes exhibited tissue-specific expression, and their reversible expression profiles responded to exogenous GA<sub>3</sub> depending on the treatment stage. We also found that the <i>DELLA</i> genes showed distinct expression patterns in the two varieties of flowering Chinese cabbage. <i>BcRGL1</i> may play a major role in the early bud differentiation process of different varieties, affecting bolting and flowering. Taken together, these results provide a theoretical basis for further dissecting the DELLA regulatory mechanism in the bolting and flowering of flowering Chinese cabbage.Hongling GuanXinmin HuangYunna ZhuBaoxing XieHoucheng LiuShiwei SongYanwei HaoRiyuan ChenMDPI AGarticleflowering Chinese cabbageDELLAgibberellin signalingbolting and floweringflower bud differentiationBiology (General)QH301-705.5ChemistryQD1-999ENInternational Journal of Molecular Sciences, Vol 22, Iss 12092, p 12092 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	flowering Chinese cabbage DELLA gibberellin signaling bolting and flowering flower bud differentiation Biology (General) QH301-705.5 Chemistry QD1-999
spellingShingle	flowering Chinese cabbage DELLA gibberellin signaling bolting and flowering flower bud differentiation Biology (General) QH301-705.5 Chemistry QD1-999 Hongling Guan Xinmin Huang Yunna Zhu Baoxing Xie Houcheng Liu Shiwei Song Yanwei Hao Riyuan Chen Identification of <i>DELLA</i> Genes and Key Stage for GA Sensitivity in Bolting and Flowering of Flowering Chinese Cabbage
description	Flowering Chinese cabbage (<i>Brassica campestris</i> L. ssp. <i>chinensis</i> var. <i>utilis</i> Tsen et Lee) is an important and extensively cultivated vegetable in south China, and its stalk development is mainly regulated by gibberellin (GA). DELLA proteins negatively regulate GA signal transduction and may play an important role in determining bolting and flowering. Nevertheless, no systematic study of the <i>DELLA</i> gene family has been undertaken in flowering Chinese cabbage. In the present study, we found that the two-true-leaf spraying of gibberellin A3 (GA<sub>3</sub>) did not promote bolting but did promote flowering, whereas the three-true-leaf spraying of GA<sub>3</sub> promoted both bolting and flowering. In addition, we identified five <i>DELLA</i> genes in flowering Chinese cabbage. All five proteins contained DELLA, VHYNP, VHIID, and SAW conserved domains. Protein-protein interaction results showed that in the presence of GA<sub>3</sub>, all five DELLA proteins interacted with BcGID1b (GA-INSENSITIVE DWARF 1b) but not with BcGID1a (GA-INSENSITIVE DWARF 1a) or BcGID1c (GA-INSENSITIVE DWARF 1c). Their expression analysis showed that the <i>DELLA</i> genes exhibited tissue-specific expression, and their reversible expression profiles responded to exogenous GA<sub>3</sub> depending on the treatment stage. We also found that the <i>DELLA</i> genes showed distinct expression patterns in the two varieties of flowering Chinese cabbage. <i>BcRGL1</i> may play a major role in the early bud differentiation process of different varieties, affecting bolting and flowering. Taken together, these results provide a theoretical basis for further dissecting the DELLA regulatory mechanism in the bolting and flowering of flowering Chinese cabbage.
format	article
author	Hongling Guan Xinmin Huang Yunna Zhu Baoxing Xie Houcheng Liu Shiwei Song Yanwei Hao Riyuan Chen
author_facet	Hongling Guan Xinmin Huang Yunna Zhu Baoxing Xie Houcheng Liu Shiwei Song Yanwei Hao Riyuan Chen
author_sort	Hongling Guan
title	Identification of <i>DELLA</i> Genes and Key Stage for GA Sensitivity in Bolting and Flowering of Flowering Chinese Cabbage
title_short	Identification of <i>DELLA</i> Genes and Key Stage for GA Sensitivity in Bolting and Flowering of Flowering Chinese Cabbage
title_full	Identification of <i>DELLA</i> Genes and Key Stage for GA Sensitivity in Bolting and Flowering of Flowering Chinese Cabbage
title_fullStr	Identification of <i>DELLA</i> Genes and Key Stage for GA Sensitivity in Bolting and Flowering of Flowering Chinese Cabbage
title_full_unstemmed	Identification of <i>DELLA</i> Genes and Key Stage for GA Sensitivity in Bolting and Flowering of Flowering Chinese Cabbage
title_sort	identification of <i>della</i> genes and key stage for ga sensitivity in bolting and flowering of flowering chinese cabbage
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/62fe863b7fb64d658aaff1a598799801
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Identification of <i>DELLA</i> Genes and Key Stage for GA Sensitivity in Bolting and Flowering of Flowering Chinese Cabbage

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