Sinomenine Hydrochloride Ameliorates Fish Foodborne Enteritis via α7nAchR-Mediated Anti-Inflammatory Effect Whilst Altering Microbiota Composition

Foodborne intestinal inflammation is a major health and welfare issue in aquaculture. To prevent enteritis, various additives have been incorporated into the fish diet. Considering anti-inflammatory immune regulation, an effective natural compound could potentially treat or prevent intestinal inflam...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Jiayuan Xie, Ming Li, Weidong Ye, Junwei Shan, Xuyang Zhao, You Duan, Yuhang Liu, Bruno Hamish Unger, Yingyin Cheng, Wanting Zhang, Nan Wu, Xiao-Qin Xia
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	SBMIE zebrafish sinomenine hydrochloride α7nAChR anti-inflammation microbiota Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/6307ceca52c24c70b20d162dac865e18
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:6307ceca52c24c70b20d162dac865e18
record_format	dspace
spelling	oai:doaj.org-article:6307ceca52c24c70b20d162dac865e182021-11-30T13:21:02ZSinomenine Hydrochloride Ameliorates Fish Foodborne Enteritis via α7nAchR-Mediated Anti-Inflammatory Effect Whilst Altering Microbiota Composition1664-322410.3389/fimmu.2021.766845https://doaj.org/article/6307ceca52c24c70b20d162dac865e182021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fimmu.2021.766845/fullhttps://doaj.org/toc/1664-3224Foodborne intestinal inflammation is a major health and welfare issue in aquaculture. To prevent enteritis, various additives have been incorporated into the fish diet. Considering anti-inflammatory immune regulation, an effective natural compound could potentially treat or prevent intestinal inflammation. Our previous study has revealed galantamine’s effect on soybean induced enteritis (SBMIE) and has highlighted the possible role of the cholinergic anti-inflammatory pathway in the fish gut. To further activate the intestinal cholinergic related anti-inflammatory function, α7nAchR signaling was considered. In this study, sinomenine, a typical agonist of α7nAChR in mammals, was tested to treat fish foodborne enteritis via its potential anti-inflammation effect using the zebrafish foodborne enteritis model. After sinomenine’s dietary inclusion, results suggested that there was an alleviation of intestinal inflammation at a pathological level. This outcome was demonstrated through the improved morphology of intestinal villi. At a molecular level, SN suppressed inflammatory cytokines’ expression (especially for tnf-α) and upregulated anti-inflammation-related functions (indicated by expression of il-10, il-22, and foxp3a). To systematically understand sinomenine’s intestinal effect on SBMIE, transcriptomic analysis was done on the SBMIE adult fish model. DEGs (sinomenine vs soybean meal groups) were enriched in GO terms related to the negative regulation of lymphocyte/leukocyte activation and alpha-beta T cell proliferation, as well as the regulation of lymphocyte migration. The KEGG pathways for glycolysis and insulin signaling indicated metabolic adjustments of α7nAchR mediated anti-inflammatory effect. To demonstrate the immune cells’ response, in the SBMIE larva model, inflammatory gatherings of neutrophils, macrophages, and lymphocytes caused by soybean meal could be relieved significantly with the inclusion of sinomenine. This was consistent within the sinomenine group as CD4+ or Foxp3+ lymphocytes were found with a higher proportion at the base of mucosal folds, which may suggest the Treg population. Echoing, the sinomenine group’s 16s sequencing result, there were fewer enteritis-related TM7, Sphingomonas and Shigella, but more Cetobacterium, which were related to glucose metabolism. Our findings indicate that sinomenine hydrochloride could be important in the prevention of fish foodborne enteritis at both immune and microbiota levels.Jiayuan XieJiayuan XieMing LiMing LiWeidong YeWeidong YeJunwei ShanJunwei ShanXuyang ZhaoXuyang ZhaoYou DuanYou DuanYuhang LiuYuhang LiuBruno Hamish UngerYingyin ChengWanting ZhangNan WuNan WuXiao-Qin XiaXiao-Qin XiaFrontiers Media S.A.articleSBMIEzebrafishsinomenine hydrochlorideα7nAChRanti-inflammationmicrobiotaImmunologic diseases. AllergyRC581-607ENFrontiers in Immunology, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	SBMIE zebrafish sinomenine hydrochloride α7nAChR anti-inflammation microbiota Immunologic diseases. Allergy RC581-607
spellingShingle	SBMIE zebrafish sinomenine hydrochloride α7nAChR anti-inflammation microbiota Immunologic diseases. Allergy RC581-607 Jiayuan Xie Jiayuan Xie Ming Li Ming Li Weidong Ye Weidong Ye Junwei Shan Junwei Shan Xuyang Zhao Xuyang Zhao You Duan You Duan Yuhang Liu Yuhang Liu Bruno Hamish Unger Yingyin Cheng Wanting Zhang Nan Wu Nan Wu Xiao-Qin Xia Xiao-Qin Xia Sinomenine Hydrochloride Ameliorates Fish Foodborne Enteritis via α7nAchR-Mediated Anti-Inflammatory Effect Whilst Altering Microbiota Composition
description	Foodborne intestinal inflammation is a major health and welfare issue in aquaculture. To prevent enteritis, various additives have been incorporated into the fish diet. Considering anti-inflammatory immune regulation, an effective natural compound could potentially treat or prevent intestinal inflammation. Our previous study has revealed galantamine’s effect on soybean induced enteritis (SBMIE) and has highlighted the possible role of the cholinergic anti-inflammatory pathway in the fish gut. To further activate the intestinal cholinergic related anti-inflammatory function, α7nAchR signaling was considered. In this study, sinomenine, a typical agonist of α7nAChR in mammals, was tested to treat fish foodborne enteritis via its potential anti-inflammation effect using the zebrafish foodborne enteritis model. After sinomenine’s dietary inclusion, results suggested that there was an alleviation of intestinal inflammation at a pathological level. This outcome was demonstrated through the improved morphology of intestinal villi. At a molecular level, SN suppressed inflammatory cytokines’ expression (especially for tnf-α) and upregulated anti-inflammation-related functions (indicated by expression of il-10, il-22, and foxp3a). To systematically understand sinomenine’s intestinal effect on SBMIE, transcriptomic analysis was done on the SBMIE adult fish model. DEGs (sinomenine vs soybean meal groups) were enriched in GO terms related to the negative regulation of lymphocyte/leukocyte activation and alpha-beta T cell proliferation, as well as the regulation of lymphocyte migration. The KEGG pathways for glycolysis and insulin signaling indicated metabolic adjustments of α7nAchR mediated anti-inflammatory effect. To demonstrate the immune cells’ response, in the SBMIE larva model, inflammatory gatherings of neutrophils, macrophages, and lymphocytes caused by soybean meal could be relieved significantly with the inclusion of sinomenine. This was consistent within the sinomenine group as CD4+ or Foxp3+ lymphocytes were found with a higher proportion at the base of mucosal folds, which may suggest the Treg population. Echoing, the sinomenine group’s 16s sequencing result, there were fewer enteritis-related TM7, Sphingomonas and Shigella, but more Cetobacterium, which were related to glucose metabolism. Our findings indicate that sinomenine hydrochloride could be important in the prevention of fish foodborne enteritis at both immune and microbiota levels.
format	article
author	Jiayuan Xie Jiayuan Xie Ming Li Ming Li Weidong Ye Weidong Ye Junwei Shan Junwei Shan Xuyang Zhao Xuyang Zhao You Duan You Duan Yuhang Liu Yuhang Liu Bruno Hamish Unger Yingyin Cheng Wanting Zhang Nan Wu Nan Wu Xiao-Qin Xia Xiao-Qin Xia
author_facet	Jiayuan Xie Jiayuan Xie Ming Li Ming Li Weidong Ye Weidong Ye Junwei Shan Junwei Shan Xuyang Zhao Xuyang Zhao You Duan You Duan Yuhang Liu Yuhang Liu Bruno Hamish Unger Yingyin Cheng Wanting Zhang Nan Wu Nan Wu Xiao-Qin Xia Xiao-Qin Xia
author_sort	Jiayuan Xie
title	Sinomenine Hydrochloride Ameliorates Fish Foodborne Enteritis via α7nAchR-Mediated Anti-Inflammatory Effect Whilst Altering Microbiota Composition
title_short	Sinomenine Hydrochloride Ameliorates Fish Foodborne Enteritis via α7nAchR-Mediated Anti-Inflammatory Effect Whilst Altering Microbiota Composition
title_full	Sinomenine Hydrochloride Ameliorates Fish Foodborne Enteritis via α7nAchR-Mediated Anti-Inflammatory Effect Whilst Altering Microbiota Composition
title_fullStr	Sinomenine Hydrochloride Ameliorates Fish Foodborne Enteritis via α7nAchR-Mediated Anti-Inflammatory Effect Whilst Altering Microbiota Composition
title_full_unstemmed	Sinomenine Hydrochloride Ameliorates Fish Foodborne Enteritis via α7nAchR-Mediated Anti-Inflammatory Effect Whilst Altering Microbiota Composition
title_sort	sinomenine hydrochloride ameliorates fish foodborne enteritis via α7nachr-mediated anti-inflammatory effect whilst altering microbiota composition
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/6307ceca52c24c70b20d162dac865e18
work_keys_str_mv	AT jiayuanxie sinomeninehydrochlorideamelioratesfishfoodborneenteritisviaa7nachrmediatedantiinflammatoryeffectwhilstalteringmicrobiotacomposition AT jiayuanxie sinomeninehydrochlorideamelioratesfishfoodborneenteritisviaa7nachrmediatedantiinflammatoryeffectwhilstalteringmicrobiotacomposition AT mingli sinomeninehydrochlorideamelioratesfishfoodborneenteritisviaa7nachrmediatedantiinflammatoryeffectwhilstalteringmicrobiotacomposition AT mingli sinomeninehydrochlorideamelioratesfishfoodborneenteritisviaa7nachrmediatedantiinflammatoryeffectwhilstalteringmicrobiotacomposition AT weidongye sinomeninehydrochlorideamelioratesfishfoodborneenteritisviaa7nachrmediatedantiinflammatoryeffectwhilstalteringmicrobiotacomposition AT weidongye sinomeninehydrochlorideamelioratesfishfoodborneenteritisviaa7nachrmediatedantiinflammatoryeffectwhilstalteringmicrobiotacomposition AT junweishan sinomeninehydrochlorideamelioratesfishfoodborneenteritisviaa7nachrmediatedantiinflammatoryeffectwhilstalteringmicrobiotacomposition AT junweishan sinomeninehydrochlorideamelioratesfishfoodborneenteritisviaa7nachrmediatedantiinflammatoryeffectwhilstalteringmicrobiotacomposition AT xuyangzhao sinomeninehydrochlorideamelioratesfishfoodborneenteritisviaa7nachrmediatedantiinflammatoryeffectwhilstalteringmicrobiotacomposition AT xuyangzhao sinomeninehydrochlorideamelioratesfishfoodborneenteritisviaa7nachrmediatedantiinflammatoryeffectwhilstalteringmicrobiotacomposition AT youduan sinomeninehydrochlorideamelioratesfishfoodborneenteritisviaa7nachrmediatedantiinflammatoryeffectwhilstalteringmicrobiotacomposition AT youduan sinomeninehydrochlorideamelioratesfishfoodborneenteritisviaa7nachrmediatedantiinflammatoryeffectwhilstalteringmicrobiotacomposition AT yuhangliu sinomeninehydrochlorideamelioratesfishfoodborneenteritisviaa7nachrmediatedantiinflammatoryeffectwhilstalteringmicrobiotacomposition AT yuhangliu sinomeninehydrochlorideamelioratesfishfoodborneenteritisviaa7nachrmediatedantiinflammatoryeffectwhilstalteringmicrobiotacomposition AT brunohamishunger sinomeninehydrochlorideamelioratesfishfoodborneenteritisviaa7nachrmediatedantiinflammatoryeffectwhilstalteringmicrobiotacomposition AT yingyincheng sinomeninehydrochlorideamelioratesfishfoodborneenteritisviaa7nachrmediatedantiinflammatoryeffectwhilstalteringmicrobiotacomposition AT wantingzhang sinomeninehydrochlorideamelioratesfishfoodborneenteritisviaa7nachrmediatedantiinflammatoryeffectwhilstalteringmicrobiotacomposition AT nanwu sinomeninehydrochlorideamelioratesfishfoodborneenteritisviaa7nachrmediatedantiinflammatoryeffectwhilstalteringmicrobiotacomposition AT nanwu sinomeninehydrochlorideamelioratesfishfoodborneenteritisviaa7nachrmediatedantiinflammatoryeffectwhilstalteringmicrobiotacomposition AT xiaoqinxia sinomeninehydrochlorideamelioratesfishfoodborneenteritisviaa7nachrmediatedantiinflammatoryeffectwhilstalteringmicrobiotacomposition AT xiaoqinxia sinomeninehydrochlorideamelioratesfishfoodborneenteritisviaa7nachrmediatedantiinflammatoryeffectwhilstalteringmicrobiotacomposition
_version_	1718406566240059392

Sinomenine Hydrochloride Ameliorates Fish Foodborne Enteritis via α7nAchR-Mediated Anti-Inflammatory Effect Whilst Altering Microbiota Composition

Ejemplares similares