First microsatellite markers for the European Robin (Erithacus rubecula) and their application in analysis of parentage and genetic diversity

Abstract The European Robin is a small passerine bird associated with woodlands of Eurasia and North Africa. Despite being relatively widespread and common, little is known of the species’ breeding biology and genetic diversity. We used Next Generation Sequencing (NGS) to develop and characterize mi...

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Autores principales:	Aleksandra Gwiazdowska, Oliwia Karpińska, Katarzyna Kamionka-Kanclerska, Patryk Rowiński, Hanna Panagiotopoulou, Jan J. Pomorski, Richard K. Broughton, Luis F. P. da Silva, Robert Rutkowski
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/6314be22749146d0b990dcc858088224
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spelling	oai:doaj.org-article:6314be22749146d0b990dcc8580882242021-12-02T18:48:23ZFirst microsatellite markers for the European Robin (Erithacus rubecula) and their application in analysis of parentage and genetic diversity10.1038/s41598-021-98364-32045-2322https://doaj.org/article/6314be22749146d0b990dcc8580882242021-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-98364-3https://doaj.org/toc/2045-2322Abstract The European Robin is a small passerine bird associated with woodlands of Eurasia and North Africa. Despite being relatively widespread and common, little is known of the species’ breeding biology and genetic diversity. We used Next Generation Sequencing (NGS) to develop and characterize microsatellite markers for the European Robin, designing three multiplex panels to amplify 14 microsatellite loci. The level of polymorphism and its value for assessing parentage and genetic structure was estimated based on 119 individuals, including seven full families and 69 unrelated individuals form Poland’s Białowieża Primaeval Forest and an additional location in Portugal. All markers appeared to be highly variable. Analysis at the family level confirmed a Mendelian manner of inheritance in the investigated loci. Genetic data also revealed evidence for extra-pair paternity in one family. The set of markers that we developed are proven to be valuable for analysis of the breeding biology and population genetics of the European Robin.Aleksandra GwiazdowskaOliwia KarpińskaKatarzyna Kamionka-KanclerskaPatryk RowińskiHanna PanagiotopoulouJan J. PomorskiRichard K. BroughtonLuis F. P. da SilvaRobert RutkowskiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Aleksandra Gwiazdowska Oliwia Karpińska Katarzyna Kamionka-Kanclerska Patryk Rowiński Hanna Panagiotopoulou Jan J. Pomorski Richard K. Broughton Luis F. P. da Silva Robert Rutkowski First microsatellite markers for the European Robin (Erithacus rubecula) and their application in analysis of parentage and genetic diversity
description	Abstract The European Robin is a small passerine bird associated with woodlands of Eurasia and North Africa. Despite being relatively widespread and common, little is known of the species’ breeding biology and genetic diversity. We used Next Generation Sequencing (NGS) to develop and characterize microsatellite markers for the European Robin, designing three multiplex panels to amplify 14 microsatellite loci. The level of polymorphism and its value for assessing parentage and genetic structure was estimated based on 119 individuals, including seven full families and 69 unrelated individuals form Poland’s Białowieża Primaeval Forest and an additional location in Portugal. All markers appeared to be highly variable. Analysis at the family level confirmed a Mendelian manner of inheritance in the investigated loci. Genetic data also revealed evidence for extra-pair paternity in one family. The set of markers that we developed are proven to be valuable for analysis of the breeding biology and population genetics of the European Robin.
format	article
author	Aleksandra Gwiazdowska Oliwia Karpińska Katarzyna Kamionka-Kanclerska Patryk Rowiński Hanna Panagiotopoulou Jan J. Pomorski Richard K. Broughton Luis F. P. da Silva Robert Rutkowski
author_facet	Aleksandra Gwiazdowska Oliwia Karpińska Katarzyna Kamionka-Kanclerska Patryk Rowiński Hanna Panagiotopoulou Jan J. Pomorski Richard K. Broughton Luis F. P. da Silva Robert Rutkowski
author_sort	Aleksandra Gwiazdowska
title	First microsatellite markers for the European Robin (Erithacus rubecula) and their application in analysis of parentage and genetic diversity
title_short	First microsatellite markers for the European Robin (Erithacus rubecula) and their application in analysis of parentage and genetic diversity
title_full	First microsatellite markers for the European Robin (Erithacus rubecula) and their application in analysis of parentage and genetic diversity
title_fullStr	First microsatellite markers for the European Robin (Erithacus rubecula) and their application in analysis of parentage and genetic diversity
title_full_unstemmed	First microsatellite markers for the European Robin (Erithacus rubecula) and their application in analysis of parentage and genetic diversity
title_sort	first microsatellite markers for the european robin (erithacus rubecula) and their application in analysis of parentage and genetic diversity
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/6314be22749146d0b990dcc858088224
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First microsatellite markers for the European Robin (Erithacus rubecula) and their application in analysis of parentage and genetic diversity

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