Formulation and characterization of chlorhexidine HCl nanoemulsion as a promising antibacterial root canal irrigant: in-vitro and ex-vivo studies

Rehab Abdelmonem,1 Mona K Younis,1 Doaa H Hassan,1 Mohamed Abd El-Gawad El-Sayed Ahmed,2 Ehab Hassanein,3 Kariem El-Batouty,3 Alaa Elfaham31Department of Pharmaceutics and Industrial Pharmacy, Faculty of Pharmacy, Misr University for Science and Technology, 6th of October City, Egypt; 2Department of...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Abdelmonem R, Younis MK, Hassan DH, El-Sayed Ahmed MAEG, Hassanien E, El-Batouty K, Elfaham A
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2019
Materias:	Chlorhexidine Hydrochloride Nanoemulsion root canal irrigant penetration ability cleansing effect antibacterial irrigant Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/6315264160f84ffe9c7d00940d3f8d8a
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:6315264160f84ffe9c7d00940d3f8d8a
record_format	dspace
spelling	oai:doaj.org-article:6315264160f84ffe9c7d00940d3f8d8a2021-12-02T08:07:08ZFormulation and characterization of chlorhexidine HCl nanoemulsion as a promising antibacterial root canal irrigant: in-vitro and ex-vivo studies1178-2013https://doaj.org/article/6315264160f84ffe9c7d00940d3f8d8a2019-07-01T00:00:00Zhttps://www.dovepress.com/formulation-and-characterization-of-chlorhexidine-hcl-nanoemulsion-as--peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Rehab Abdelmonem,1 Mona K Younis,1 Doaa H Hassan,1 Mohamed Abd El-Gawad El-Sayed Ahmed,2 Ehab Hassanein,3 Kariem El-Batouty,3 Alaa Elfaham31Department of Pharmaceutics and Industrial Pharmacy, Faculty of Pharmacy, Misr University for Science and Technology, 6th of October City, Egypt; 2Department of Microbiology and Immunology, Faculty of Pharmacy, Misr University for Science and Technology, 6th of October City, Egypt; 3Department of Endodontics Faculty of Dentistry, Ain Shams University, Cairo, EgyptIntroduction and aim: Chlorhexidine Hydrochloride [Chx.HCl] has a broad-spectrum antibacterial effect, sustained action and low toxicity so it has been recommended as a potential root canal irrigant. The aim of this study was to improve the penetration ability, cleansing and antibacterial effect of Chx.HCl using a newly formulated Chx.HCl nanoemulsion and use it as root canal irrigant.Methods: Chx.HCl nanoemulsions were prepared using two different oils; Oleic acid and Labrafil M1944CS, two surfactants; Tween 20 and Tween 80 and co-surfactant; Propylene Glycol. Pseudoternary phase diagrams were constructed to designate the optimum systems. The prepared nanoemulsion formulae were evaluated for their drug content, emulsification time, dispersibility, droplet size, in-vitro drug release, thermodynamic stability, In-vitro antibacterial activity and ex-vivo study for the selected formula. Comparisons were made of Chx.HCl nanoemulsion with two different concentrations 0.75% and 1.6% vs Chx.HCl normal particle size as root canal irrigant for their penetration ability, cleansing effect and antibacterial effect.Results: The selected formula was F6 with composition of 2% Labrafil, 12% Tween 80 and 6% Propylene glycol. It has small particle size (12.18 nm), short emulsification time (1.67 seconds), and fast dissolution rate after 2 minutes. It was found to be a thermodynamically/physically stable system. The higher concentration of Chx.HClnanoemulsion1.6% shows the best penetration ability compared to Chx.HCl normal particle size due to the smaller particle size. Chx.HCl nanoemulsion 1.6% has the lowest mean value of the remaining debris surface area (2001.47 μm2,) when compared to normal particle size material (2609.56 μm2,).Conclusion: Chx.HCl nanoemulsion preparation has better cleansing ability and antibacterial effect with high efficacy on Enterococcus faecalis, where high reduction rate or complete eradication of bacterial cells has been achieved.Keywords: chlorhexidine hydrochloride, nanoemulsion, root canal irrigant, penetration ability, cleansing effect, antibacterial irrigantAbdelmonem RYounis MKHassan DHEl-Sayed Ahmed MAEGHassanien EEl-Batouty KElfaham ADove Medical PressarticleChlorhexidine HydrochlorideNanoemulsionroot canal irrigantpenetration abilitycleansing effectantibacterial irrigantMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 14, Pp 4697-4708 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Chlorhexidine Hydrochloride Nanoemulsion root canal irrigant penetration ability cleansing effect antibacterial irrigant Medicine (General) R5-920
spellingShingle	Chlorhexidine Hydrochloride Nanoemulsion root canal irrigant penetration ability cleansing effect antibacterial irrigant Medicine (General) R5-920 Abdelmonem R Younis MK Hassan DH El-Sayed Ahmed MAEG Hassanien E El-Batouty K Elfaham A Formulation and characterization of chlorhexidine HCl nanoemulsion as a promising antibacterial root canal irrigant: in-vitro and ex-vivo studies
description	Rehab Abdelmonem,1 Mona K Younis,1 Doaa H Hassan,1 Mohamed Abd El-Gawad El-Sayed Ahmed,2 Ehab Hassanein,3 Kariem El-Batouty,3 Alaa Elfaham31Department of Pharmaceutics and Industrial Pharmacy, Faculty of Pharmacy, Misr University for Science and Technology, 6th of October City, Egypt; 2Department of Microbiology and Immunology, Faculty of Pharmacy, Misr University for Science and Technology, 6th of October City, Egypt; 3Department of Endodontics Faculty of Dentistry, Ain Shams University, Cairo, EgyptIntroduction and aim: Chlorhexidine Hydrochloride [Chx.HCl] has a broad-spectrum antibacterial effect, sustained action and low toxicity so it has been recommended as a potential root canal irrigant. The aim of this study was to improve the penetration ability, cleansing and antibacterial effect of Chx.HCl using a newly formulated Chx.HCl nanoemulsion and use it as root canal irrigant.Methods: Chx.HCl nanoemulsions were prepared using two different oils; Oleic acid and Labrafil M1944CS, two surfactants; Tween 20 and Tween 80 and co-surfactant; Propylene Glycol. Pseudoternary phase diagrams were constructed to designate the optimum systems. The prepared nanoemulsion formulae were evaluated for their drug content, emulsification time, dispersibility, droplet size, in-vitro drug release, thermodynamic stability, In-vitro antibacterial activity and ex-vivo study for the selected formula. Comparisons were made of Chx.HCl nanoemulsion with two different concentrations 0.75% and 1.6% vs Chx.HCl normal particle size as root canal irrigant for their penetration ability, cleansing effect and antibacterial effect.Results: The selected formula was F6 with composition of 2% Labrafil, 12% Tween 80 and 6% Propylene glycol. It has small particle size (12.18 nm), short emulsification time (1.67 seconds), and fast dissolution rate after 2 minutes. It was found to be a thermodynamically/physically stable system. The higher concentration of Chx.HClnanoemulsion1.6% shows the best penetration ability compared to Chx.HCl normal particle size due to the smaller particle size. Chx.HCl nanoemulsion 1.6% has the lowest mean value of the remaining debris surface area (2001.47 μm2,) when compared to normal particle size material (2609.56 μm2,).Conclusion: Chx.HCl nanoemulsion preparation has better cleansing ability and antibacterial effect with high efficacy on Enterococcus faecalis, where high reduction rate or complete eradication of bacterial cells has been achieved.Keywords: chlorhexidine hydrochloride, nanoemulsion, root canal irrigant, penetration ability, cleansing effect, antibacterial irrigant
format	article
author	Abdelmonem R Younis MK Hassan DH El-Sayed Ahmed MAEG Hassanien E El-Batouty K Elfaham A
author_facet	Abdelmonem R Younis MK Hassan DH El-Sayed Ahmed MAEG Hassanien E El-Batouty K Elfaham A
author_sort	Abdelmonem R
title	Formulation and characterization of chlorhexidine HCl nanoemulsion as a promising antibacterial root canal irrigant: in-vitro and ex-vivo studies
title_short	Formulation and characterization of chlorhexidine HCl nanoemulsion as a promising antibacterial root canal irrigant: in-vitro and ex-vivo studies
title_full	Formulation and characterization of chlorhexidine HCl nanoemulsion as a promising antibacterial root canal irrigant: in-vitro and ex-vivo studies
title_fullStr	Formulation and characterization of chlorhexidine HCl nanoemulsion as a promising antibacterial root canal irrigant: in-vitro and ex-vivo studies
title_full_unstemmed	Formulation and characterization of chlorhexidine HCl nanoemulsion as a promising antibacterial root canal irrigant: in-vitro and ex-vivo studies
title_sort	formulation and characterization of chlorhexidine hcl nanoemulsion as a promising antibacterial root canal irrigant: in-vitro and ex-vivo studies
publisher	Dove Medical Press
publishDate	2019
url	https://doaj.org/article/6315264160f84ffe9c7d00940d3f8d8a
work_keys_str_mv	AT abdelmonemr formulationandcharacterizationofchlorhexidinehclnanoemulsionasapromisingantibacterialrootcanalirrigantinvitroandexvivostudies AT younismk formulationandcharacterizationofchlorhexidinehclnanoemulsionasapromisingantibacterialrootcanalirrigantinvitroandexvivostudies AT hassandh formulationandcharacterizationofchlorhexidinehclnanoemulsionasapromisingantibacterialrootcanalirrigantinvitroandexvivostudies AT elsayedahmedmaeg formulationandcharacterizationofchlorhexidinehclnanoemulsionasapromisingantibacterialrootcanalirrigantinvitroandexvivostudies AT hassaniene formulationandcharacterizationofchlorhexidinehclnanoemulsionasapromisingantibacterialrootcanalirrigantinvitroandexvivostudies AT elbatoutyk formulationandcharacterizationofchlorhexidinehclnanoemulsionasapromisingantibacterialrootcanalirrigantinvitroandexvivostudies AT elfahama formulationandcharacterizationofchlorhexidinehclnanoemulsionasapromisingantibacterialrootcanalirrigantinvitroandexvivostudies
_version_	1718398664407252992

Formulation and characterization of chlorhexidine HCl nanoemulsion as a promising antibacterial root canal irrigant: in-vitro and ex-vivo studies

Ejemplares similares