Short Communication: Isolation and characterization of thermophilic actinobacteria as proteolytic enzyme producer from Ie Seuum Hot Spring, Aceh Besar, Indonesia

Abstract. Fitri L, Putri KA, Suhartono, Ismail YS. 2019. Short Communication: Isolation and characterization of thermophilic actinobacteria as proteolytic enzyme producer from Ie Seuum Hot Spring, Aceh Besar, Indonesia. Biodiversitas 20: 2802-2808. Proteases are enzymes who catalyze the breakdown of...

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Autores principales:	Lenni Fitri, KARTINI AMELIA PUTRI, SUHARTONO, YULIA SARI ISMAIL
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2019
Materias:	actinobacteria, hot spring, ie seu'um, protease Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/63165cb13d1946be87b8dbd50298a09d
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spelling	oai:doaj.org-article:63165cb13d1946be87b8dbd50298a09d2021-11-21T21:54:58ZShort Communication: Isolation and characterization of thermophilic actinobacteria as proteolytic enzyme producer from Ie Seuum Hot Spring, Aceh Besar, Indonesia1412-033X2085-472210.13057/biodiv/d201004https://doaj.org/article/63165cb13d1946be87b8dbd50298a09d2019-09-01T00:00:00Zhttps://smujo.id/biodiv/article/view/4200https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Abstract. Fitri L, Putri KA, Suhartono, Ismail YS. 2019. Short Communication: Isolation and characterization of thermophilic actinobacteria as proteolytic enzyme producer from Ie Seuum Hot Spring, Aceh Besar, Indonesia. Biodiversitas 20: 2802-2808. Proteases are enzymes who catalyze the breakdown of peptide bonds in proteins. This enzyme could be produced from thermophilic bacteria that are able to grow at temperatures of 45-80ºC and are stable to heat. The samples were collected at Ie Seu'um hot spring, Aceh Besar. This study aimed to isolate, to characterize and to determine actinobacteria that were capable of producing protease enzymes. The sampling method in this study was conducted by purposive sampling at temperatures of 50, 60 and 70ºC. Isolation of thermophilic actinobacteria was carried out in Humic Acid Vitamin B (HV) Agar medium and morphological characterization was carried out in Yeast Malt Agar (YMA), Yeast Starch Agar (YSA), Oatmeal Agar (OA) media. Microscopic characterization and measurement of clear zone diameter formed were carried out in skim milk medium. The results showed that one actinobacteria isolate was obtained at a temperature of 50ºC and five isolates at a temperature of 60ºC, meanwhile, no actinobacteria could be obtained at a temperature of 70ºC. A total of 4 isolates obtained were able to produce protease enzymes. The highest Proteolytic Index (IP) value was obtained from IS01 which was 3.8.Lenni FitriKARTINI AMELIA PUTRISUHARTONOYULIA SARI ISMAILMBI & UNS Soloarticleactinobacteria, hot spring, ie seu'um, proteaseBiology (General)QH301-705.5ENBiodiversitas, Vol 20, Iss 10 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	actinobacteria, hot spring, ie seu'um, protease Biology (General) QH301-705.5
spellingShingle	actinobacteria, hot spring, ie seu'um, protease Biology (General) QH301-705.5 Lenni Fitri KARTINI AMELIA PUTRI SUHARTONO YULIA SARI ISMAIL Short Communication: Isolation and characterization of thermophilic actinobacteria as proteolytic enzyme producer from Ie Seuum Hot Spring, Aceh Besar, Indonesia
description	Abstract. Fitri L, Putri KA, Suhartono, Ismail YS. 2019. Short Communication: Isolation and characterization of thermophilic actinobacteria as proteolytic enzyme producer from Ie Seuum Hot Spring, Aceh Besar, Indonesia. Biodiversitas 20: 2802-2808. Proteases are enzymes who catalyze the breakdown of peptide bonds in proteins. This enzyme could be produced from thermophilic bacteria that are able to grow at temperatures of 45-80ºC and are stable to heat. The samples were collected at Ie Seu'um hot spring, Aceh Besar. This study aimed to isolate, to characterize and to determine actinobacteria that were capable of producing protease enzymes. The sampling method in this study was conducted by purposive sampling at temperatures of 50, 60 and 70ºC. Isolation of thermophilic actinobacteria was carried out in Humic Acid Vitamin B (HV) Agar medium and morphological characterization was carried out in Yeast Malt Agar (YMA), Yeast Starch Agar (YSA), Oatmeal Agar (OA) media. Microscopic characterization and measurement of clear zone diameter formed were carried out in skim milk medium. The results showed that one actinobacteria isolate was obtained at a temperature of 50ºC and five isolates at a temperature of 60ºC, meanwhile, no actinobacteria could be obtained at a temperature of 70ºC. A total of 4 isolates obtained were able to produce protease enzymes. The highest Proteolytic Index (IP) value was obtained from IS01 which was 3.8.
format	article
author	Lenni Fitri KARTINI AMELIA PUTRI SUHARTONO YULIA SARI ISMAIL
author_facet	Lenni Fitri KARTINI AMELIA PUTRI SUHARTONO YULIA SARI ISMAIL
author_sort	Lenni Fitri
title	Short Communication: Isolation and characterization of thermophilic actinobacteria as proteolytic enzyme producer from Ie Seuum Hot Spring, Aceh Besar, Indonesia
title_short	Short Communication: Isolation and characterization of thermophilic actinobacteria as proteolytic enzyme producer from Ie Seuum Hot Spring, Aceh Besar, Indonesia
title_full	Short Communication: Isolation and characterization of thermophilic actinobacteria as proteolytic enzyme producer from Ie Seuum Hot Spring, Aceh Besar, Indonesia
title_fullStr	Short Communication: Isolation and characterization of thermophilic actinobacteria as proteolytic enzyme producer from Ie Seuum Hot Spring, Aceh Besar, Indonesia
title_full_unstemmed	Short Communication: Isolation and characterization of thermophilic actinobacteria as proteolytic enzyme producer from Ie Seuum Hot Spring, Aceh Besar, Indonesia
title_sort	short communication: isolation and characterization of thermophilic actinobacteria as proteolytic enzyme producer from ie seuum hot spring, aceh besar, indonesia
publisher	MBI & UNS Solo
publishDate	2019
url	https://doaj.org/article/63165cb13d1946be87b8dbd50298a09d
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_version_	1718418645137227776

Short Communication: Isolation and characterization of thermophilic actinobacteria as proteolytic enzyme producer from Ie Seuum Hot Spring, Aceh Besar, Indonesia

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