Comparison of RT-PCR, RT-nested PCRs, and real-time PCR for diagnosis of severe fever with thrombocytopenia syndrome: a prospective study

Abstract We designed a highly sensitive reverse transcription nested polymerase chain reaction targeting the M-segment (NPCR-M) of severe fever with thrombocytopenia syndrome (SFTS) virus. NPCR-M was performed in parallel with three other referenced PCR assays QPCR-S, PCR-M, and NPCR-S to assess the...

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Autores principales:	Sehrish Jalal, Seong Yeon Hwang, Choon-Mee Kim, Dong-Min Kim, Na Ra Yun, Jun-Won Seo, Da Young Kim, Sook In Jung, Uh Jin Kim, Seong Eun Kim, Hyun ah Kim, Eu Suk Kim, Jian Hur, Young Keun Kim, Hye Won Jeong, Jung Yeon Heo, Dong Sik Jung, Jieun Kim, Sun Hee Park, Yee Gyung Kwak, Sujin Lee, Seungjin Lim, Sun Hee Lee
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/63181c256f424ea1b7cbdc5ba2e2b42c
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spelling	oai:doaj.org-article:63181c256f424ea1b7cbdc5ba2e2b42c2021-12-02T16:46:35ZComparison of RT-PCR, RT-nested PCRs, and real-time PCR for diagnosis of severe fever with thrombocytopenia syndrome: a prospective study10.1038/s41598-021-96066-42045-2322https://doaj.org/article/63181c256f424ea1b7cbdc5ba2e2b42c2021-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-96066-4https://doaj.org/toc/2045-2322Abstract We designed a highly sensitive reverse transcription nested polymerase chain reaction targeting the M-segment (NPCR-M) of severe fever with thrombocytopenia syndrome (SFTS) virus. NPCR-M was performed in parallel with three other referenced PCR assays QPCR-S, PCR-M, and NPCR-S to assess their clinical usefulness as routine diagnostic techniques for SFTS. In this multi-centered prospective study, 122 blood samples from 38 laboratory-confirmed SFTS patients and 85 control samples were used. The results demonstrated that QPCR-S and NPCR-S had better sensitivity rate up to 21 days after symptom onset however, the PCR-M showed poor sensitivity after 7 days of symptom onset. Our designed NPCR-M had a higher detection rate up to 40 days from symptom onset and revealed the persistence of SFTSV RNA in the early convalescent phase. No false-positive results were seen for the control samples. Additionally, NPCR-M showed positive results for a sample that initially showed negative results from other PCRs and for many other samples collected in the convalescent phase of SFTS. Our designed nested PCR is suitable for SFTSV detection in patients’ blood collected in the acute and early convalescent phase of SFTS, and shows better sensitivity and high specificity even up to 40 days after symptom onset.Sehrish JalalSeong Yeon HwangChoon-Mee KimDong-Min KimNa Ra YunJun-Won SeoDa Young KimSook In JungUh Jin KimSeong Eun KimHyun ah KimEu Suk KimJian HurYoung Keun KimHye Won JeongJung Yeon HeoDong Sik JungJieun KimSun Hee ParkYee Gyung KwakSujin LeeSeungjin LimSun Hee LeeNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-8 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Sehrish Jalal Seong Yeon Hwang Choon-Mee Kim Dong-Min Kim Na Ra Yun Jun-Won Seo Da Young Kim Sook In Jung Uh Jin Kim Seong Eun Kim Hyun ah Kim Eu Suk Kim Jian Hur Young Keun Kim Hye Won Jeong Jung Yeon Heo Dong Sik Jung Jieun Kim Sun Hee Park Yee Gyung Kwak Sujin Lee Seungjin Lim Sun Hee Lee Comparison of RT-PCR, RT-nested PCRs, and real-time PCR for diagnosis of severe fever with thrombocytopenia syndrome: a prospective study
description	Abstract We designed a highly sensitive reverse transcription nested polymerase chain reaction targeting the M-segment (NPCR-M) of severe fever with thrombocytopenia syndrome (SFTS) virus. NPCR-M was performed in parallel with three other referenced PCR assays QPCR-S, PCR-M, and NPCR-S to assess their clinical usefulness as routine diagnostic techniques for SFTS. In this multi-centered prospective study, 122 blood samples from 38 laboratory-confirmed SFTS patients and 85 control samples were used. The results demonstrated that QPCR-S and NPCR-S had better sensitivity rate up to 21 days after symptom onset however, the PCR-M showed poor sensitivity after 7 days of symptom onset. Our designed NPCR-M had a higher detection rate up to 40 days from symptom onset and revealed the persistence of SFTSV RNA in the early convalescent phase. No false-positive results were seen for the control samples. Additionally, NPCR-M showed positive results for a sample that initially showed negative results from other PCRs and for many other samples collected in the convalescent phase of SFTS. Our designed nested PCR is suitable for SFTSV detection in patients’ blood collected in the acute and early convalescent phase of SFTS, and shows better sensitivity and high specificity even up to 40 days after symptom onset.
format	article
author	Sehrish Jalal Seong Yeon Hwang Choon-Mee Kim Dong-Min Kim Na Ra Yun Jun-Won Seo Da Young Kim Sook In Jung Uh Jin Kim Seong Eun Kim Hyun ah Kim Eu Suk Kim Jian Hur Young Keun Kim Hye Won Jeong Jung Yeon Heo Dong Sik Jung Jieun Kim Sun Hee Park Yee Gyung Kwak Sujin Lee Seungjin Lim Sun Hee Lee
author_facet	Sehrish Jalal Seong Yeon Hwang Choon-Mee Kim Dong-Min Kim Na Ra Yun Jun-Won Seo Da Young Kim Sook In Jung Uh Jin Kim Seong Eun Kim Hyun ah Kim Eu Suk Kim Jian Hur Young Keun Kim Hye Won Jeong Jung Yeon Heo Dong Sik Jung Jieun Kim Sun Hee Park Yee Gyung Kwak Sujin Lee Seungjin Lim Sun Hee Lee
author_sort	Sehrish Jalal
title	Comparison of RT-PCR, RT-nested PCRs, and real-time PCR for diagnosis of severe fever with thrombocytopenia syndrome: a prospective study
title_short	Comparison of RT-PCR, RT-nested PCRs, and real-time PCR for diagnosis of severe fever with thrombocytopenia syndrome: a prospective study
title_full	Comparison of RT-PCR, RT-nested PCRs, and real-time PCR for diagnosis of severe fever with thrombocytopenia syndrome: a prospective study
title_fullStr	Comparison of RT-PCR, RT-nested PCRs, and real-time PCR for diagnosis of severe fever with thrombocytopenia syndrome: a prospective study
title_full_unstemmed	Comparison of RT-PCR, RT-nested PCRs, and real-time PCR for diagnosis of severe fever with thrombocytopenia syndrome: a prospective study
title_sort	comparison of rt-pcr, rt-nested pcrs, and real-time pcr for diagnosis of severe fever with thrombocytopenia syndrome: a prospective study
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/63181c256f424ea1b7cbdc5ba2e2b42c
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Comparison of RT-PCR, RT-nested PCRs, and real-time PCR for diagnosis of severe fever with thrombocytopenia syndrome: a prospective study

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