Molecular and immunological characterization of ragweed (Ambrosia artemisiifolia L.) pollen after exposure of the plants to elevated ozone over a whole growing season.

Climate change and air pollution, including ozone is known to affect plants and might also influence the ragweed pollen, known to carry strong allergens. We compared the transcriptome of ragweed pollen produced under ambient and elevated ozone by 454-sequencing. An enzyme-linked immunosorbent assay...

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Autores principales:	Ulrike Kanter, Werner Heller, Jörg Durner, J Barbro Winkler, Marion Engel, Heidrun Behrendt, Andreas Holzinger, Paula Braun, Michael Hauser, Fatima Ferreira, Klaus Mayer, Matthias Pfeifer, Dieter Ernst
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/63288efdad2047b78e7efdc9e7d10cd4
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spelling	oai:doaj.org-article:63288efdad2047b78e7efdc9e7d10cd42021-11-18T07:48:53ZMolecular and immunological characterization of ragweed (Ambrosia artemisiifolia L.) pollen after exposure of the plants to elevated ozone over a whole growing season.1932-620310.1371/journal.pone.0061518https://doaj.org/article/63288efdad2047b78e7efdc9e7d10cd42013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23637846/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Climate change and air pollution, including ozone is known to affect plants and might also influence the ragweed pollen, known to carry strong allergens. We compared the transcriptome of ragweed pollen produced under ambient and elevated ozone by 454-sequencing. An enzyme-linked immunosorbent assay (ELISA) was carried out for the major ragweed allergen Amb a 1. Pollen surface was examined by scanning electron microscopy and attenuated total reflectance-Fourier transform infrared spectroscopy (ATR-FTIR), and phenolics were analysed by high-performance liquid chromatography. Elevated ozone had no influence on the pollen size, shape, surface structure or amount of phenolics. ATR-FTIR indicated increased pectin-like material in the exine. Transcriptomic analyses showed changes in expressed-sequence tags (ESTs), including allergens. However, ELISA indicated no significantly increased amounts of Amb a 1 under elevated ozone concentrations. The data highlight a direct influence of ozone on the exine components and transcript level of allergens. As the total protein amount of Amb a 1 was not altered, a direct correlation to an increased risk to human health could not be derived. Additional, the 454-sequencing contributes to the identification of stress-related transcripts in mature pollen that could be grouped into distinct gene ontology terms.Ulrike KanterWerner HellerJörg DurnerJ Barbro WinklerMarion EngelHeidrun BehrendtAndreas HolzingerPaula BraunMichael HauserFatima FerreiraKlaus MayerMatthias PfeiferDieter ErnstPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 4, p e61518 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ulrike Kanter Werner Heller Jörg Durner J Barbro Winkler Marion Engel Heidrun Behrendt Andreas Holzinger Paula Braun Michael Hauser Fatima Ferreira Klaus Mayer Matthias Pfeifer Dieter Ernst Molecular and immunological characterization of ragweed (Ambrosia artemisiifolia L.) pollen after exposure of the plants to elevated ozone over a whole growing season.
description	Climate change and air pollution, including ozone is known to affect plants and might also influence the ragweed pollen, known to carry strong allergens. We compared the transcriptome of ragweed pollen produced under ambient and elevated ozone by 454-sequencing. An enzyme-linked immunosorbent assay (ELISA) was carried out for the major ragweed allergen Amb a 1. Pollen surface was examined by scanning electron microscopy and attenuated total reflectance-Fourier transform infrared spectroscopy (ATR-FTIR), and phenolics were analysed by high-performance liquid chromatography. Elevated ozone had no influence on the pollen size, shape, surface structure or amount of phenolics. ATR-FTIR indicated increased pectin-like material in the exine. Transcriptomic analyses showed changes in expressed-sequence tags (ESTs), including allergens. However, ELISA indicated no significantly increased amounts of Amb a 1 under elevated ozone concentrations. The data highlight a direct influence of ozone on the exine components and transcript level of allergens. As the total protein amount of Amb a 1 was not altered, a direct correlation to an increased risk to human health could not be derived. Additional, the 454-sequencing contributes to the identification of stress-related transcripts in mature pollen that could be grouped into distinct gene ontology terms.
format	article
author	Ulrike Kanter Werner Heller Jörg Durner J Barbro Winkler Marion Engel Heidrun Behrendt Andreas Holzinger Paula Braun Michael Hauser Fatima Ferreira Klaus Mayer Matthias Pfeifer Dieter Ernst
author_facet	Ulrike Kanter Werner Heller Jörg Durner J Barbro Winkler Marion Engel Heidrun Behrendt Andreas Holzinger Paula Braun Michael Hauser Fatima Ferreira Klaus Mayer Matthias Pfeifer Dieter Ernst
author_sort	Ulrike Kanter
title	Molecular and immunological characterization of ragweed (Ambrosia artemisiifolia L.) pollen after exposure of the plants to elevated ozone over a whole growing season.
title_short	Molecular and immunological characterization of ragweed (Ambrosia artemisiifolia L.) pollen after exposure of the plants to elevated ozone over a whole growing season.
title_full	Molecular and immunological characterization of ragweed (Ambrosia artemisiifolia L.) pollen after exposure of the plants to elevated ozone over a whole growing season.
title_fullStr	Molecular and immunological characterization of ragweed (Ambrosia artemisiifolia L.) pollen after exposure of the plants to elevated ozone over a whole growing season.
title_full_unstemmed	Molecular and immunological characterization of ragweed (Ambrosia artemisiifolia L.) pollen after exposure of the plants to elevated ozone over a whole growing season.
title_sort	molecular and immunological characterization of ragweed (ambrosia artemisiifolia l.) pollen after exposure of the plants to elevated ozone over a whole growing season.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/63288efdad2047b78e7efdc9e7d10cd4
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Molecular and immunological characterization of ragweed (Ambrosia artemisiifolia L.) pollen after exposure of the plants to elevated ozone over a whole growing season.

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