Anti-EpCAM functionalized graphene oxide vector for tumor targeted siRNA delivery and cancer therapy

Graphene oxide (GO) has emerged as a potential drug delivery vector. For siRNA delivery, GO should be modified to endow it with gene delivery ability and targeting effect. However, the cationic materials used previously usually had greater toxicity. In this study, GO was modified with a non-toxicity...

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Autores principales:	Si Chen, Shuang Zhang, Yifan Wang, Xin Yang, Hong Yang, Chunying Cui
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Graphene oxide siRNA delivery Survivin Anti-EpCAM Gene silencing Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/632eaaf289344daab5d921fabaee017f
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spelling	oai:doaj.org-article:632eaaf289344daab5d921fabaee017f2021-11-20T04:57:41ZAnti-EpCAM functionalized graphene oxide vector for tumor targeted siRNA delivery and cancer therapy1818-087610.1016/j.ajps.2021.04.002https://doaj.org/article/632eaaf289344daab5d921fabaee017f2021-09-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S1818087621000374https://doaj.org/toc/1818-0876Graphene oxide (GO) has emerged as a potential drug delivery vector. For siRNA delivery, GO should be modified to endow it with gene delivery ability and targeting effect. However, the cationic materials used previously usually had greater toxicity. In this study, GO was modified with a non-toxicity cationic material (chitosan) and a tumor specific monoclonal antibody (anti-EpCAM) for the delivery of survivin-siRNA (GCE/siRNA). And the vector (GCE) prepared was proved with excellent biosafety and tumor targeting effect. The GCE exhibited superior performance in loading siRNA, maintained stability in different solutions and showed excellent protection effect for survivin-siRNA in vitro. The gene silencing results in vitro showed that the mRNA level and protein level were down-regulated by 48.24% ± 2.50% and 44.12% ± 3.03%, respectively, which was equal with positive control (P > 0.05). It was also demonstrated that GCE/siRNA had a strong antitumor effect in vitro, which was attributed to the efficient antiproliferation, and migration and invasion inhibition effect of GCE/siRNA. The results in vivo indicated that GCE could accumulate siRNA in tumor tissues. The tumor inhibition rate of GCE/siRNA 54.74% ± 5.51% was significantly higher than control 4.87% ± 8.49%. Moreover, GCE/siRNA showed no toxicity for blood and main organs, suggesting that it is a biosafety carrier for gene delivery. Taken together, this study provides a novel design strategy for gene delivery system and siRNA formulation.Si ChenShuang ZhangYifan WangXin YangHong YangChunying CuiElsevierarticleGraphene oxidesiRNA deliverySurvivinAnti-EpCAMGene silencingTherapeutics. PharmacologyRM1-950ENAsian Journal of Pharmaceutical Sciences, Vol 16, Iss 5, Pp 598-611 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Graphene oxide siRNA delivery Survivin Anti-EpCAM Gene silencing Therapeutics. Pharmacology RM1-950
spellingShingle	Graphene oxide siRNA delivery Survivin Anti-EpCAM Gene silencing Therapeutics. Pharmacology RM1-950 Si Chen Shuang Zhang Yifan Wang Xin Yang Hong Yang Chunying Cui Anti-EpCAM functionalized graphene oxide vector for tumor targeted siRNA delivery and cancer therapy
description	Graphene oxide (GO) has emerged as a potential drug delivery vector. For siRNA delivery, GO should be modified to endow it with gene delivery ability and targeting effect. However, the cationic materials used previously usually had greater toxicity. In this study, GO was modified with a non-toxicity cationic material (chitosan) and a tumor specific monoclonal antibody (anti-EpCAM) for the delivery of survivin-siRNA (GCE/siRNA). And the vector (GCE) prepared was proved with excellent biosafety and tumor targeting effect. The GCE exhibited superior performance in loading siRNA, maintained stability in different solutions and showed excellent protection effect for survivin-siRNA in vitro. The gene silencing results in vitro showed that the mRNA level and protein level were down-regulated by 48.24% ± 2.50% and 44.12% ± 3.03%, respectively, which was equal with positive control (P > 0.05). It was also demonstrated that GCE/siRNA had a strong antitumor effect in vitro, which was attributed to the efficient antiproliferation, and migration and invasion inhibition effect of GCE/siRNA. The results in vivo indicated that GCE could accumulate siRNA in tumor tissues. The tumor inhibition rate of GCE/siRNA 54.74% ± 5.51% was significantly higher than control 4.87% ± 8.49%. Moreover, GCE/siRNA showed no toxicity for blood and main organs, suggesting that it is a biosafety carrier for gene delivery. Taken together, this study provides a novel design strategy for gene delivery system and siRNA formulation.
format	article
author	Si Chen Shuang Zhang Yifan Wang Xin Yang Hong Yang Chunying Cui
author_facet	Si Chen Shuang Zhang Yifan Wang Xin Yang Hong Yang Chunying Cui
author_sort	Si Chen
title	Anti-EpCAM functionalized graphene oxide vector for tumor targeted siRNA delivery and cancer therapy
title_short	Anti-EpCAM functionalized graphene oxide vector for tumor targeted siRNA delivery and cancer therapy
title_full	Anti-EpCAM functionalized graphene oxide vector for tumor targeted siRNA delivery and cancer therapy
title_fullStr	Anti-EpCAM functionalized graphene oxide vector for tumor targeted siRNA delivery and cancer therapy
title_full_unstemmed	Anti-EpCAM functionalized graphene oxide vector for tumor targeted siRNA delivery and cancer therapy
title_sort	anti-epcam functionalized graphene oxide vector for tumor targeted sirna delivery and cancer therapy
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/632eaaf289344daab5d921fabaee017f
work_keys_str_mv	AT sichen antiepcamfunctionalizedgrapheneoxidevectorfortumortargetedsirnadeliveryandcancertherapy AT shuangzhang antiepcamfunctionalizedgrapheneoxidevectorfortumortargetedsirnadeliveryandcancertherapy AT yifanwang antiepcamfunctionalizedgrapheneoxidevectorfortumortargetedsirnadeliveryandcancertherapy AT xinyang antiepcamfunctionalizedgrapheneoxidevectorfortumortargetedsirnadeliveryandcancertherapy AT hongyang antiepcamfunctionalizedgrapheneoxidevectorfortumortargetedsirnadeliveryandcancertherapy AT chunyingcui antiepcamfunctionalizedgrapheneoxidevectorfortumortargetedsirnadeliveryandcancertherapy
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Anti-EpCAM functionalized graphene oxide vector for tumor targeted siRNA delivery and cancer therapy

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