On mechanism behind UV-A light enhanced antibacterial activity of gallic acid and propyl gallate against Escherichia coli O157:H7

Abstract Possible mechanisms behind the enhanced antimicrobial activity of gallic acid (GA) and its ester propyl gallate (PG) in the presence of UV-A light against Escherichia coli O157:H7 were investigated. GA by itself is a mild antimicrobial and has a pro-oxidant ability. We found that the presen...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Qingyang Wang, Erick Falcao de Oliveira, Solmaz Alborzi, Luis J. Bastarrachea, Rohan V. Tikekar
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/63359a91de974370b2a1352d160e0131
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:63359a91de974370b2a1352d160e0131
record_format	dspace
spelling	oai:doaj.org-article:63359a91de974370b2a1352d160e01312021-12-02T11:52:15ZOn mechanism behind UV-A light enhanced antibacterial activity of gallic acid and propyl gallate against Escherichia coli O157:H710.1038/s41598-017-08449-12045-2322https://doaj.org/article/63359a91de974370b2a1352d160e01312017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-08449-1https://doaj.org/toc/2045-2322Abstract Possible mechanisms behind the enhanced antimicrobial activity of gallic acid (GA) and its ester propyl gallate (PG) in the presence of UV-A light against Escherichia coli O157:H7 were investigated. GA by itself is a mild antimicrobial and has a pro-oxidant ability. We found that the presence of UV-A light increases the uptake of GA by the bacteria. Once GA is internalized, the interaction between GA and UV-A induces intracellular ROS formation, leading to oxidative damage. Concurrently, GA + UV-A also inhibits the activity of superoxide dismutase (SOD), magnifying the imbalance of redox status of E. coli O157:H7. In addition to ROS induced damage, UV-A light and GA also cause injury to the cell membrane of E. coli O157:H7. UV-A exposed PG caused oxidative damage to the cell and significantly higher damage to the cell membrane than GA + UV-A treatment, explaining its higher effectiveness than GA + UV-A treatment. The findings presented here may be useful in developing new antimicrobial sanitation technologies for food and pharmaceutical industries.Qingyang WangErick Falcao de OliveiraSolmaz AlborziLuis J. BastarracheaRohan V. TikekarNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Qingyang Wang Erick Falcao de Oliveira Solmaz Alborzi Luis J. Bastarrachea Rohan V. Tikekar On mechanism behind UV-A light enhanced antibacterial activity of gallic acid and propyl gallate against Escherichia coli O157:H7
description	Abstract Possible mechanisms behind the enhanced antimicrobial activity of gallic acid (GA) and its ester propyl gallate (PG) in the presence of UV-A light against Escherichia coli O157:H7 were investigated. GA by itself is a mild antimicrobial and has a pro-oxidant ability. We found that the presence of UV-A light increases the uptake of GA by the bacteria. Once GA is internalized, the interaction between GA and UV-A induces intracellular ROS formation, leading to oxidative damage. Concurrently, GA + UV-A also inhibits the activity of superoxide dismutase (SOD), magnifying the imbalance of redox status of E. coli O157:H7. In addition to ROS induced damage, UV-A light and GA also cause injury to the cell membrane of E. coli O157:H7. UV-A exposed PG caused oxidative damage to the cell and significantly higher damage to the cell membrane than GA + UV-A treatment, explaining its higher effectiveness than GA + UV-A treatment. The findings presented here may be useful in developing new antimicrobial sanitation technologies for food and pharmaceutical industries.
format	article
author	Qingyang Wang Erick Falcao de Oliveira Solmaz Alborzi Luis J. Bastarrachea Rohan V. Tikekar
author_facet	Qingyang Wang Erick Falcao de Oliveira Solmaz Alborzi Luis J. Bastarrachea Rohan V. Tikekar
author_sort	Qingyang Wang
title	On mechanism behind UV-A light enhanced antibacterial activity of gallic acid and propyl gallate against Escherichia coli O157:H7
title_short	On mechanism behind UV-A light enhanced antibacterial activity of gallic acid and propyl gallate against Escherichia coli O157:H7
title_full	On mechanism behind UV-A light enhanced antibacterial activity of gallic acid and propyl gallate against Escherichia coli O157:H7
title_fullStr	On mechanism behind UV-A light enhanced antibacterial activity of gallic acid and propyl gallate against Escherichia coli O157:H7
title_full_unstemmed	On mechanism behind UV-A light enhanced antibacterial activity of gallic acid and propyl gallate against Escherichia coli O157:H7
title_sort	on mechanism behind uv-a light enhanced antibacterial activity of gallic acid and propyl gallate against escherichia coli o157:h7
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/63359a91de974370b2a1352d160e0131
work_keys_str_mv	AT qingyangwang onmechanismbehinduvalightenhancedantibacterialactivityofgallicacidandpropylgallateagainstescherichiacolio157h7 AT erickfalcaodeoliveira onmechanismbehinduvalightenhancedantibacterialactivityofgallicacidandpropylgallateagainstescherichiacolio157h7 AT solmazalborzi onmechanismbehinduvalightenhancedantibacterialactivityofgallicacidandpropylgallateagainstescherichiacolio157h7 AT luisjbastarrachea onmechanismbehinduvalightenhancedantibacterialactivityofgallicacidandpropylgallateagainstescherichiacolio157h7 AT rohanvtikekar onmechanismbehinduvalightenhancedantibacterialactivityofgallicacidandpropylgallateagainstescherichiacolio157h7
_version_	1718395107053404160

On mechanism behind UV-A light enhanced antibacterial activity of gallic acid and propyl gallate against Escherichia coli O157:H7

Ejemplares similares