Kinetic and Interaction Studies of Adenosine-5′-Triphosphate (ATP) Hydrolysis with Polyoxovanadates

The reactivity of polyoxovanadates towards adenosine-5′-triphosphate (ATP) hydrolysis at pH 2, 4, 6 and 7 is reported. Detailed kinetic investigation of ATP hydrolysis in the presence of polyoxovanadates was performed through multinuclear nuclear magnetic resonance (NMR) spectroscopy. In general, ra...

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Autores principales:	Francisco de Azambuja, Nele Steens, Tatjana N. Parac-Vogt
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	vanadium ATP hydrolysis polyoxovanadates polyoxometalates Mining engineering. Metallurgy TN1-997
Acceso en línea:	https://doaj.org/article/6339ea9d25b74fa584eecda341f8200f
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spelling	oai:doaj.org-article:6339ea9d25b74fa584eecda341f8200f2021-11-25T18:21:09ZKinetic and Interaction Studies of Adenosine-5′-Triphosphate (ATP) Hydrolysis with Polyoxovanadates10.3390/met111116782075-4701https://doaj.org/article/6339ea9d25b74fa584eecda341f8200f2021-10-01T00:00:00Zhttps://www.mdpi.com/2075-4701/11/11/1678https://doaj.org/toc/2075-4701The reactivity of polyoxovanadates towards adenosine-5′-triphosphate (ATP) hydrolysis at pH 2, 4, 6 and 7 is reported. Detailed kinetic investigation of ATP hydrolysis in the presence of polyoxovanadates was performed through multinuclear nuclear magnetic resonance (NMR) spectroscopy. In general, rate acceleration of up to five orders of magnitude was observed in the presence of vanadates compared to spontaneous ATP hydrolysis, with the greatest acceleration observed for reactions carried out at pH 2. Interestingly, the effectiveness of vanadates in promoting ATP hydrolysis decreased as the pH of the reaction solution increased; nevertheless, at pH = 7, the rate increase of one order of magnitude in comparison to blank reactions was still observed. Interactions between vanadate species in solution and ATP were investigated by means of <sup>31</sup>P and <sup>51</sup>V NMR spectroscopy, and this pointed towards the preferential interaction of vanadium with the phosphate groups rather than other regions of the ATP molecule.Francisco de AzambujaNele SteensTatjana N. Parac-VogtMDPI AGarticlevanadiumATPhydrolysispolyoxovanadatespolyoxometalatesMining engineering. MetallurgyTN1-997ENMetals, Vol 11, Iss 1678, p 1678 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	vanadium ATP hydrolysis polyoxovanadates polyoxometalates Mining engineering. Metallurgy TN1-997
spellingShingle	vanadium ATP hydrolysis polyoxovanadates polyoxometalates Mining engineering. Metallurgy TN1-997 Francisco de Azambuja Nele Steens Tatjana N. Parac-Vogt Kinetic and Interaction Studies of Adenosine-5′-Triphosphate (ATP) Hydrolysis with Polyoxovanadates
description	The reactivity of polyoxovanadates towards adenosine-5′-triphosphate (ATP) hydrolysis at pH 2, 4, 6 and 7 is reported. Detailed kinetic investigation of ATP hydrolysis in the presence of polyoxovanadates was performed through multinuclear nuclear magnetic resonance (NMR) spectroscopy. In general, rate acceleration of up to five orders of magnitude was observed in the presence of vanadates compared to spontaneous ATP hydrolysis, with the greatest acceleration observed for reactions carried out at pH 2. Interestingly, the effectiveness of vanadates in promoting ATP hydrolysis decreased as the pH of the reaction solution increased; nevertheless, at pH = 7, the rate increase of one order of magnitude in comparison to blank reactions was still observed. Interactions between vanadate species in solution and ATP were investigated by means of <sup>31</sup>P and <sup>51</sup>V NMR spectroscopy, and this pointed towards the preferential interaction of vanadium with the phosphate groups rather than other regions of the ATP molecule.
format	article
author	Francisco de Azambuja Nele Steens Tatjana N. Parac-Vogt
author_facet	Francisco de Azambuja Nele Steens Tatjana N. Parac-Vogt
author_sort	Francisco de Azambuja
title	Kinetic and Interaction Studies of Adenosine-5′-Triphosphate (ATP) Hydrolysis with Polyoxovanadates
title_short	Kinetic and Interaction Studies of Adenosine-5′-Triphosphate (ATP) Hydrolysis with Polyoxovanadates
title_full	Kinetic and Interaction Studies of Adenosine-5′-Triphosphate (ATP) Hydrolysis with Polyoxovanadates
title_fullStr	Kinetic and Interaction Studies of Adenosine-5′-Triphosphate (ATP) Hydrolysis with Polyoxovanadates
title_full_unstemmed	Kinetic and Interaction Studies of Adenosine-5′-Triphosphate (ATP) Hydrolysis with Polyoxovanadates
title_sort	kinetic and interaction studies of adenosine-5′-triphosphate (atp) hydrolysis with polyoxovanadates
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/6339ea9d25b74fa584eecda341f8200f
work_keys_str_mv	AT franciscodeazambuja kineticandinteractionstudiesofadenosine5triphosphateatphydrolysiswithpolyoxovanadates AT nelesteens kineticandinteractionstudiesofadenosine5triphosphateatphydrolysiswithpolyoxovanadates AT tatjananparacvogt kineticandinteractionstudiesofadenosine5triphosphateatphydrolysiswithpolyoxovanadates
_version_	1718411260597370880

Kinetic and Interaction Studies of Adenosine-5′-Triphosphate (ATP) Hydrolysis with Polyoxovanadates

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