Functional role of CD40 and CD154 costimulatory signals in IgZ-mediated immunity against bacterial infection
CD40 and CD154 are one of the best-characterized costimulatory molecules essential for adaptive immunity, which extensively involved in T and B cell activation, IgM Ab production, isotype class switching, germinal center formation and affinity maturation. However, the functionality of CD40 and CD154...
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2021
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oai:doaj.org-article:633b795073464be594dcb17b8416ba312021-11-26T04:42:32ZFunctional role of CD40 and CD154 costimulatory signals in IgZ-mediated immunity against bacterial infection2667-011910.1016/j.fsirep.2021.100038https://doaj.org/article/633b795073464be594dcb17b8416ba312021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2667011921000335https://doaj.org/toc/2667-0119CD40 and CD154 are one of the best-characterized costimulatory molecules essential for adaptive immunity, which extensively involved in T and B cell activation, IgM Ab production, isotype class switching, germinal center formation and affinity maturation. However, the functionality of CD40 and CD154 in IgZ-mediated immunity remains limited. In this study, we explored the regulatory role of Cd40-Cd154 interaction in IgZ-mediated antibacterial immunity in zebrafish. The results showed that the IgZ-mediated antibacterial response can be significantly induced in response to A. hydrophila infection. The percentage of Cd40+IgZ+ B cells and the production of IgZ Ab were substantially increased upon A. hydrophila stimulation, but these reactions were markedly declined in Cd154 blockade fish by administering anti-Cd154 Ab or recombinant sCd40-Ig protein, accompanied with the impairment of the vaccine-initiated IgZ-mediated immunoprotection of fish against A. hydrophila infection. These observations suggested the essential role of Cd40-Cd154 interaction in IgZ-mediated bacterial immunity. Notably, the Cd40 and Cd154 costimulatory signals are required for a TD antigen-induced IgZ immunity, but are not indispensable for a TI antigen-induced IgZ immune response. These findings indicated the differential role of Cd40-Cd154 interaction in bacterial TD and TI antigen-induced IgZ immunity, which suggested the existence of diverse regulatory mechanisms underlying IgZ-mediated antibacterial immune reactions. To our knowledge, this is the first report to show the functional role of Cd40-Cd154 costimulatory signaling pathway in IgZ-mediated immune defense against bacterial infection. We hope this study will improve the current understanding of the coevolution between the IgZ/IgT immunoglobins and CD40/CD154 costimulatory molecules.Ning SuChong-bin HuTong ShaoChun-yu JinHao LiJian-fei JiLu-lu QinDong-Dong FanAi-fu LinLi-xin XiangJian-zhong ShaoElsevierarticleCD40CD154IgZCostimulatory regulationAntibacterial immunityZoologyQL1-991ENFish and Shellfish Immunology Reports, Vol 2, Iss , Pp 100038- (2021) |
| institution |
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| collection |
DOAJ |
| language |
EN |
| topic |
CD40 CD154 IgZ Costimulatory regulation Antibacterial immunity Zoology QL1-991 |
| spellingShingle |
CD40 CD154 IgZ Costimulatory regulation Antibacterial immunity Zoology QL1-991 Ning Su Chong-bin Hu Tong Shao Chun-yu Jin Hao Li Jian-fei Ji Lu-lu Qin Dong-Dong Fan Ai-fu Lin Li-xin Xiang Jian-zhong Shao Functional role of CD40 and CD154 costimulatory signals in IgZ-mediated immunity against bacterial infection |
| description |
CD40 and CD154 are one of the best-characterized costimulatory molecules essential for adaptive immunity, which extensively involved in T and B cell activation, IgM Ab production, isotype class switching, germinal center formation and affinity maturation. However, the functionality of CD40 and CD154 in IgZ-mediated immunity remains limited. In this study, we explored the regulatory role of Cd40-Cd154 interaction in IgZ-mediated antibacterial immunity in zebrafish. The results showed that the IgZ-mediated antibacterial response can be significantly induced in response to A. hydrophila infection. The percentage of Cd40+IgZ+ B cells and the production of IgZ Ab were substantially increased upon A. hydrophila stimulation, but these reactions were markedly declined in Cd154 blockade fish by administering anti-Cd154 Ab or recombinant sCd40-Ig protein, accompanied with the impairment of the vaccine-initiated IgZ-mediated immunoprotection of fish against A. hydrophila infection. These observations suggested the essential role of Cd40-Cd154 interaction in IgZ-mediated bacterial immunity. Notably, the Cd40 and Cd154 costimulatory signals are required for a TD antigen-induced IgZ immunity, but are not indispensable for a TI antigen-induced IgZ immune response. These findings indicated the differential role of Cd40-Cd154 interaction in bacterial TD and TI antigen-induced IgZ immunity, which suggested the existence of diverse regulatory mechanisms underlying IgZ-mediated antibacterial immune reactions. To our knowledge, this is the first report to show the functional role of Cd40-Cd154 costimulatory signaling pathway in IgZ-mediated immune defense against bacterial infection. We hope this study will improve the current understanding of the coevolution between the IgZ/IgT immunoglobins and CD40/CD154 costimulatory molecules. |
| format |
article |
| author |
Ning Su Chong-bin Hu Tong Shao Chun-yu Jin Hao Li Jian-fei Ji Lu-lu Qin Dong-Dong Fan Ai-fu Lin Li-xin Xiang Jian-zhong Shao |
| author_facet |
Ning Su Chong-bin Hu Tong Shao Chun-yu Jin Hao Li Jian-fei Ji Lu-lu Qin Dong-Dong Fan Ai-fu Lin Li-xin Xiang Jian-zhong Shao |
| author_sort |
Ning Su |
| title |
Functional role of CD40 and CD154 costimulatory signals in IgZ-mediated immunity against bacterial infection |
| title_short |
Functional role of CD40 and CD154 costimulatory signals in IgZ-mediated immunity against bacterial infection |
| title_full |
Functional role of CD40 and CD154 costimulatory signals in IgZ-mediated immunity against bacterial infection |
| title_fullStr |
Functional role of CD40 and CD154 costimulatory signals in IgZ-mediated immunity against bacterial infection |
| title_full_unstemmed |
Functional role of CD40 and CD154 costimulatory signals in IgZ-mediated immunity against bacterial infection |
| title_sort |
functional role of cd40 and cd154 costimulatory signals in igz-mediated immunity against bacterial infection |
| publisher |
Elsevier |
| publishDate |
2021 |
| url |
https://doaj.org/article/633b795073464be594dcb17b8416ba31 |
| work_keys_str_mv |
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| _version_ |
1718409780463140864 |