Differentially expressed genes in the femur cartilage transcriptome clarify the understanding of femoral head separation in chickens

Abstract Locomotor problems are among one of the main concerns in the current poultry industry, causing major economic losses and affecting animal welfare. The most common bone anomalies in the femur are dyschondroplasia, femoral head separation (FHS), and bacterial chondronecrosis with osteomyeliti...

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Autores principales:	Ludmila Mudri Hul, Adriana Mércia Guaratini Ibelli, Igor Ricardo Savoldi, Débora Ester Petry Marcelino, Lana Teixeira Fernandes, Jane Oliveira Peixoto, Maurício Egídio Cantão, Roberto Hiroshi Higa, Poliana Fernanda Giachetto, Luiz Lehmann Coutinho, Mônica Corrêa Ledur
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/633c6a92324043269f4601f3fdee3581
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spelling	oai:doaj.org-article:633c6a92324043269f4601f3fdee35812021-12-02T14:58:46ZDifferentially expressed genes in the femur cartilage transcriptome clarify the understanding of femoral head separation in chickens10.1038/s41598-021-97306-32045-2322https://doaj.org/article/633c6a92324043269f4601f3fdee35812021-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-97306-3https://doaj.org/toc/2045-2322Abstract Locomotor problems are among one of the main concerns in the current poultry industry, causing major economic losses and affecting animal welfare. The most common bone anomalies in the femur are dyschondroplasia, femoral head separation (FHS), and bacterial chondronecrosis with osteomyelitis (BCO), also known as femoral head necrosis (FHN). The present study aimed to identify differentially expressed (DE) genes in the articular cartilage (AC) of normal and FHS-affected broilers by RNA-Seq analysis. In the transcriptome analysis, 12,169 genes were expressed in the femur AC. Of those, 107 genes were DE (FDR < 0.05) between normal and affected chickens, of which 9 were downregulated and 98 were upregulated in the affected broilers. In the gene-set enrichment analysis using the DE genes, 79 biological processes (BP) were identified and were grouped into 12 superclusters. The main BP found were involved in the response to biotic stimulus, gas transport, cellular activation, carbohydrate-derived catabolism, multi-organism regulation, immune system, muscle contraction, multi-organism process, cytolysis, leukocytes and cell adhesion. In this study, the first transcriptome analysis of the broilers femur articular cartilage was performed, and a set of candidate genes (AvBD1, AvBD2, ANK1, EPX, ADA, RHAG) that could trigger changes in the broiler´s femoral growth plate was identified. Moreover, these results could be helpful to better understand FHN in chickens and possibly in humans.Ludmila Mudri HulAdriana Mércia Guaratini IbelliIgor Ricardo SavoldiDébora Ester Petry MarcelinoLana Teixeira FernandesJane Oliveira PeixotoMaurício Egídio CantãoRoberto Hiroshi HigaPoliana Fernanda GiachettoLuiz Lehmann CoutinhoMônica Corrêa LedurNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ludmila Mudri Hul Adriana Mércia Guaratini Ibelli Igor Ricardo Savoldi Débora Ester Petry Marcelino Lana Teixeira Fernandes Jane Oliveira Peixoto Maurício Egídio Cantão Roberto Hiroshi Higa Poliana Fernanda Giachetto Luiz Lehmann Coutinho Mônica Corrêa Ledur Differentially expressed genes in the femur cartilage transcriptome clarify the understanding of femoral head separation in chickens
description	Abstract Locomotor problems are among one of the main concerns in the current poultry industry, causing major economic losses and affecting animal welfare. The most common bone anomalies in the femur are dyschondroplasia, femoral head separation (FHS), and bacterial chondronecrosis with osteomyelitis (BCO), also known as femoral head necrosis (FHN). The present study aimed to identify differentially expressed (DE) genes in the articular cartilage (AC) of normal and FHS-affected broilers by RNA-Seq analysis. In the transcriptome analysis, 12,169 genes were expressed in the femur AC. Of those, 107 genes were DE (FDR < 0.05) between normal and affected chickens, of which 9 were downregulated and 98 were upregulated in the affected broilers. In the gene-set enrichment analysis using the DE genes, 79 biological processes (BP) were identified and were grouped into 12 superclusters. The main BP found were involved in the response to biotic stimulus, gas transport, cellular activation, carbohydrate-derived catabolism, multi-organism regulation, immune system, muscle contraction, multi-organism process, cytolysis, leukocytes and cell adhesion. In this study, the first transcriptome analysis of the broilers femur articular cartilage was performed, and a set of candidate genes (AvBD1, AvBD2, ANK1, EPX, ADA, RHAG) that could trigger changes in the broiler´s femoral growth plate was identified. Moreover, these results could be helpful to better understand FHN in chickens and possibly in humans.
format	article
author	Ludmila Mudri Hul Adriana Mércia Guaratini Ibelli Igor Ricardo Savoldi Débora Ester Petry Marcelino Lana Teixeira Fernandes Jane Oliveira Peixoto Maurício Egídio Cantão Roberto Hiroshi Higa Poliana Fernanda Giachetto Luiz Lehmann Coutinho Mônica Corrêa Ledur
author_facet	Ludmila Mudri Hul Adriana Mércia Guaratini Ibelli Igor Ricardo Savoldi Débora Ester Petry Marcelino Lana Teixeira Fernandes Jane Oliveira Peixoto Maurício Egídio Cantão Roberto Hiroshi Higa Poliana Fernanda Giachetto Luiz Lehmann Coutinho Mônica Corrêa Ledur
author_sort	Ludmila Mudri Hul
title	Differentially expressed genes in the femur cartilage transcriptome clarify the understanding of femoral head separation in chickens
title_short	Differentially expressed genes in the femur cartilage transcriptome clarify the understanding of femoral head separation in chickens
title_full	Differentially expressed genes in the femur cartilage transcriptome clarify the understanding of femoral head separation in chickens
title_fullStr	Differentially expressed genes in the femur cartilage transcriptome clarify the understanding of femoral head separation in chickens
title_full_unstemmed	Differentially expressed genes in the femur cartilage transcriptome clarify the understanding of femoral head separation in chickens
title_sort	differentially expressed genes in the femur cartilage transcriptome clarify the understanding of femoral head separation in chickens
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/633c6a92324043269f4601f3fdee3581
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Differentially expressed genes in the femur cartilage transcriptome clarify the understanding of femoral head separation in chickens

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