Comparative proteomic analysis provides new insights into regulation of microspore embryogenesis induction in winter triticale (× Triticosecale Wittm.) after 5-azacytidine treatment

Abstract Effective microspore embryogenesis (ME) requires substantial modifications in gene expression pattern, followed by changes in the cell proteome and its metabolism. Recent studies have awakened also interest in the role of epigenetic factors in microspore de-differentiation and reprogramming...

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Autores principales:	Monika Krzewska, Ewa Dubas, Gabriela Gołębiowska, Anna Nowicka, Agnieszka Janas, Kamil Zieliński, Ewa Surówka, Przemysław Kopeć, Przemysław Mielczarek, Iwona Żur
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/6342f00265144ec6a2503a3c11c73b2b
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spelling	oai:doaj.org-article:6342f00265144ec6a2503a3c11c73b2b2021-11-21T12:17:23ZComparative proteomic analysis provides new insights into regulation of microspore embryogenesis induction in winter triticale (× Triticosecale Wittm.) after 5-azacytidine treatment10.1038/s41598-021-01671-y2045-2322https://doaj.org/article/6342f00265144ec6a2503a3c11c73b2b2021-11-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-01671-yhttps://doaj.org/toc/2045-2322Abstract Effective microspore embryogenesis (ME) requires substantial modifications in gene expression pattern, followed by changes in the cell proteome and its metabolism. Recent studies have awakened also interest in the role of epigenetic factors in microspore de-differentiation and reprogramming. Therefore, demethylating agent (2.5–10 μM 5-azacytidine, AC) together with low temperature (3 weeks at 4 °C) were used as ME-inducing tiller treatment in two doubled haploid (DH) lines of triticale and its effect was analyzed in respect of anther protein profiles, expression of selected genes (TAPETUM DETERMINANT1 (TaTPD1-like), SOMATIC EMBRYOGENESIS RECEPTOR KINASE 2 (SERK2) and GLUTATHIONE S-TRANSFERASE (GSTF2)) and ME efficiency. Tiller treatment with 5.0 µM AC was the most effective in ME induction; it was associated with (1) suppression of intensive anabolic processes-mainly photosynthesis and light-dependent reactions, (2) transition to effective catabolism and mobilization of carbohydrate reserve to meet the high energy demand of cells during microspore reprograming and (3) effective defense against stress-inducing treatment, i.e. protection of proper folding during protein biosynthesis and effective degradation of dysfunctional or damaged proteins. Additionally, 5.0 µM AC enhanced the expression of all genes previously identified as being associated with embryogenic potential of microspores (TaTPD1-like, SERK and GSTF2).Monika KrzewskaEwa DubasGabriela GołębiowskaAnna NowickaAgnieszka JanasKamil ZielińskiEwa SurówkaPrzemysław KopećPrzemysław MielczarekIwona ŻurNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-18 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Monika Krzewska Ewa Dubas Gabriela Gołębiowska Anna Nowicka Agnieszka Janas Kamil Zieliński Ewa Surówka Przemysław Kopeć Przemysław Mielczarek Iwona Żur Comparative proteomic analysis provides new insights into regulation of microspore embryogenesis induction in winter triticale (× Triticosecale Wittm.) after 5-azacytidine treatment
description	Abstract Effective microspore embryogenesis (ME) requires substantial modifications in gene expression pattern, followed by changes in the cell proteome and its metabolism. Recent studies have awakened also interest in the role of epigenetic factors in microspore de-differentiation and reprogramming. Therefore, demethylating agent (2.5–10 μM 5-azacytidine, AC) together with low temperature (3 weeks at 4 °C) were used as ME-inducing tiller treatment in two doubled haploid (DH) lines of triticale and its effect was analyzed in respect of anther protein profiles, expression of selected genes (TAPETUM DETERMINANT1 (TaTPD1-like), SOMATIC EMBRYOGENESIS RECEPTOR KINASE 2 (SERK2) and GLUTATHIONE S-TRANSFERASE (GSTF2)) and ME efficiency. Tiller treatment with 5.0 µM AC was the most effective in ME induction; it was associated with (1) suppression of intensive anabolic processes-mainly photosynthesis and light-dependent reactions, (2) transition to effective catabolism and mobilization of carbohydrate reserve to meet the high energy demand of cells during microspore reprograming and (3) effective defense against stress-inducing treatment, i.e. protection of proper folding during protein biosynthesis and effective degradation of dysfunctional or damaged proteins. Additionally, 5.0 µM AC enhanced the expression of all genes previously identified as being associated with embryogenic potential of microspores (TaTPD1-like, SERK and GSTF2).
format	article
author	Monika Krzewska Ewa Dubas Gabriela Gołębiowska Anna Nowicka Agnieszka Janas Kamil Zieliński Ewa Surówka Przemysław Kopeć Przemysław Mielczarek Iwona Żur
author_facet	Monika Krzewska Ewa Dubas Gabriela Gołębiowska Anna Nowicka Agnieszka Janas Kamil Zieliński Ewa Surówka Przemysław Kopeć Przemysław Mielczarek Iwona Żur
author_sort	Monika Krzewska
title	Comparative proteomic analysis provides new insights into regulation of microspore embryogenesis induction in winter triticale (× Triticosecale Wittm.) after 5-azacytidine treatment
title_short	Comparative proteomic analysis provides new insights into regulation of microspore embryogenesis induction in winter triticale (× Triticosecale Wittm.) after 5-azacytidine treatment
title_full	Comparative proteomic analysis provides new insights into regulation of microspore embryogenesis induction in winter triticale (× Triticosecale Wittm.) after 5-azacytidine treatment
title_fullStr	Comparative proteomic analysis provides new insights into regulation of microspore embryogenesis induction in winter triticale (× Triticosecale Wittm.) after 5-azacytidine treatment
title_full_unstemmed	Comparative proteomic analysis provides new insights into regulation of microspore embryogenesis induction in winter triticale (× Triticosecale Wittm.) after 5-azacytidine treatment
title_sort	comparative proteomic analysis provides new insights into regulation of microspore embryogenesis induction in winter triticale (× triticosecale wittm.) after 5-azacytidine treatment
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/6342f00265144ec6a2503a3c11c73b2b
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Comparative proteomic analysis provides new insights into regulation of microspore embryogenesis induction in winter triticale (× Triticosecale Wittm.) after 5-azacytidine treatment

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