Nerve growth factor and glutamate increase the density and expression of substance P-containing nerve fibers in healthy human masseter muscles

Abstract Nocifensive behavior induced by injection of glutamate or nerve growth factor (NGF) into rats masseter muscle is mediated, in part, through the activation of peripheral NMDA receptors. However, information is lacking about the mechanism that contributes to pain and sensitization induced by...

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Autores principales:	Abdelrahman M. Alhilou, Akiko Shimada, Camilla I. Svensson, Peter Svensson, Malin Ernberg, Brian E. Cairns, Nikolaos Christidis
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/634c70c42366403d96bbc0c09dd108cc
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spelling	oai:doaj.org-article:634c70c42366403d96bbc0c09dd108cc2021-12-02T16:35:36ZNerve growth factor and glutamate increase the density and expression of substance P-containing nerve fibers in healthy human masseter muscles10.1038/s41598-021-95229-72045-2322https://doaj.org/article/634c70c42366403d96bbc0c09dd108cc2021-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-95229-7https://doaj.org/toc/2045-2322Abstract Nocifensive behavior induced by injection of glutamate or nerve growth factor (NGF) into rats masseter muscle is mediated, in part, through the activation of peripheral NMDA receptors. However, information is lacking about the mechanism that contributes to pain and sensitization induced by these substances in humans. Immunohistochemical analysis of microbiopsies obtained from human masseter muscle was used to investigate if injection of glutamate into the NGF-sensitized masseter muscle alters the density or expression of the NMDA receptor subtype 2B (NR2B) or NGF by putative sensory afferent (that express SP) fibers. The relationship between expression and pain characteristics was also examined. NGF and glutamate administration increased the density and expression of NR2B and NGF by muscle putative sensory afferent fibers (P < 0.050). This increase in expression was greater in women than in men (P < 0.050). Expression of NR2B receptors by putative sensory afferent fibers was positively correlated with pain characteristics. Results suggest that increased expression of peripheral NMDA receptors partly contributes to the increased pain and sensitivity induced by intramuscular injection of NGF and glutamate in healthy humans; a model of myofascial temporomandibular disorder (TMD) pain. Whether a similar increase in peripheral NMDA expression occurs in patients with painful TMDs warrants further investigation.Abdelrahman M. AlhilouAkiko ShimadaCamilla I. SvenssonPeter SvenssonMalin ErnbergBrian E. CairnsNikolaos ChristidisNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Abdelrahman M. Alhilou Akiko Shimada Camilla I. Svensson Peter Svensson Malin Ernberg Brian E. Cairns Nikolaos Christidis Nerve growth factor and glutamate increase the density and expression of substance P-containing nerve fibers in healthy human masseter muscles
description	Abstract Nocifensive behavior induced by injection of glutamate or nerve growth factor (NGF) into rats masseter muscle is mediated, in part, through the activation of peripheral NMDA receptors. However, information is lacking about the mechanism that contributes to pain and sensitization induced by these substances in humans. Immunohistochemical analysis of microbiopsies obtained from human masseter muscle was used to investigate if injection of glutamate into the NGF-sensitized masseter muscle alters the density or expression of the NMDA receptor subtype 2B (NR2B) or NGF by putative sensory afferent (that express SP) fibers. The relationship between expression and pain characteristics was also examined. NGF and glutamate administration increased the density and expression of NR2B and NGF by muscle putative sensory afferent fibers (P < 0.050). This increase in expression was greater in women than in men (P < 0.050). Expression of NR2B receptors by putative sensory afferent fibers was positively correlated with pain characteristics. Results suggest that increased expression of peripheral NMDA receptors partly contributes to the increased pain and sensitivity induced by intramuscular injection of NGF and glutamate in healthy humans; a model of myofascial temporomandibular disorder (TMD) pain. Whether a similar increase in peripheral NMDA expression occurs in patients with painful TMDs warrants further investigation.
format	article
author	Abdelrahman M. Alhilou Akiko Shimada Camilla I. Svensson Peter Svensson Malin Ernberg Brian E. Cairns Nikolaos Christidis
author_facet	Abdelrahman M. Alhilou Akiko Shimada Camilla I. Svensson Peter Svensson Malin Ernberg Brian E. Cairns Nikolaos Christidis
author_sort	Abdelrahman M. Alhilou
title	Nerve growth factor and glutamate increase the density and expression of substance P-containing nerve fibers in healthy human masseter muscles
title_short	Nerve growth factor and glutamate increase the density and expression of substance P-containing nerve fibers in healthy human masseter muscles
title_full	Nerve growth factor and glutamate increase the density and expression of substance P-containing nerve fibers in healthy human masseter muscles
title_fullStr	Nerve growth factor and glutamate increase the density and expression of substance P-containing nerve fibers in healthy human masseter muscles
title_full_unstemmed	Nerve growth factor and glutamate increase the density and expression of substance P-containing nerve fibers in healthy human masseter muscles
title_sort	nerve growth factor and glutamate increase the density and expression of substance p-containing nerve fibers in healthy human masseter muscles
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/634c70c42366403d96bbc0c09dd108cc
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Nerve growth factor and glutamate increase the density and expression of substance P-containing nerve fibers in healthy human masseter muscles

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