Heterochronic shift in Hox-mediated activation of sonic hedgehog leads to morphological changes during fin development.

We explored the molecular mechanisms of morphological transformations of vertebrate paired fin/limb evolution by comparative gene expression profiling and functional analyses. In this study, we focused on the temporal differences of the onset of Sonic hedgehog (Shh) expression in paired appendages a...

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Autores principales:	Koji Sakamoto, Koh Onimaru, Keijiro Munakata, Natsuno Suda, Mika Tamura, Haruki Ochi, Mikiko Tanaka
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2009
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/634f361556e242b28f07d9b20ba75109
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spelling	oai:doaj.org-article:634f361556e242b28f07d9b20ba751092021-11-25T06:16:10ZHeterochronic shift in Hox-mediated activation of sonic hedgehog leads to morphological changes during fin development.1932-620310.1371/journal.pone.0005121https://doaj.org/article/634f361556e242b28f07d9b20ba751092009-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/19365553/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203We explored the molecular mechanisms of morphological transformations of vertebrate paired fin/limb evolution by comparative gene expression profiling and functional analyses. In this study, we focused on the temporal differences of the onset of Sonic hedgehog (Shh) expression in paired appendages among different vertebrates. In limb buds of chick and mouse, Shh expression is activated as soon as there is a morphological bud, concomitant with Hoxd10 expression. In dogfish (Scyliorhinus canicula), however, we found that Shh was transcribed late in fin development, concomitant with Hoxd13 expression. We utilized zebrafish as a model to determine whether quantitative changes in hox expression alter the timing of shh expression in pectoral fins of zebrafish embryos. We found that the temporal shift of Shh activity altered the size of endoskeletal elements in paired fins of zebrafish and dogfish. Thus, a threshold level of hox expression determines the onset of shh expression, and the subsequent heterochronic shift of Shh activity can affect the size of the fin endoskeleton. This process may have facilitated major morphological changes in paired appendages during vertebrate limb evolution.Koji SakamotoKoh OnimaruKeijiro MunakataNatsuno SudaMika TamuraHaruki OchiMikiko TanakaPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 4, Iss 4, p e5121 (2009)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Koji Sakamoto Koh Onimaru Keijiro Munakata Natsuno Suda Mika Tamura Haruki Ochi Mikiko Tanaka Heterochronic shift in Hox-mediated activation of sonic hedgehog leads to morphological changes during fin development.
description	We explored the molecular mechanisms of morphological transformations of vertebrate paired fin/limb evolution by comparative gene expression profiling and functional analyses. In this study, we focused on the temporal differences of the onset of Sonic hedgehog (Shh) expression in paired appendages among different vertebrates. In limb buds of chick and mouse, Shh expression is activated as soon as there is a morphological bud, concomitant with Hoxd10 expression. In dogfish (Scyliorhinus canicula), however, we found that Shh was transcribed late in fin development, concomitant with Hoxd13 expression. We utilized zebrafish as a model to determine whether quantitative changes in hox expression alter the timing of shh expression in pectoral fins of zebrafish embryos. We found that the temporal shift of Shh activity altered the size of endoskeletal elements in paired fins of zebrafish and dogfish. Thus, a threshold level of hox expression determines the onset of shh expression, and the subsequent heterochronic shift of Shh activity can affect the size of the fin endoskeleton. This process may have facilitated major morphological changes in paired appendages during vertebrate limb evolution.
format	article
author	Koji Sakamoto Koh Onimaru Keijiro Munakata Natsuno Suda Mika Tamura Haruki Ochi Mikiko Tanaka
author_facet	Koji Sakamoto Koh Onimaru Keijiro Munakata Natsuno Suda Mika Tamura Haruki Ochi Mikiko Tanaka
author_sort	Koji Sakamoto
title	Heterochronic shift in Hox-mediated activation of sonic hedgehog leads to morphological changes during fin development.
title_short	Heterochronic shift in Hox-mediated activation of sonic hedgehog leads to morphological changes during fin development.
title_full	Heterochronic shift in Hox-mediated activation of sonic hedgehog leads to morphological changes during fin development.
title_fullStr	Heterochronic shift in Hox-mediated activation of sonic hedgehog leads to morphological changes during fin development.
title_full_unstemmed	Heterochronic shift in Hox-mediated activation of sonic hedgehog leads to morphological changes during fin development.
title_sort	heterochronic shift in hox-mediated activation of sonic hedgehog leads to morphological changes during fin development.
publisher	Public Library of Science (PLoS)
publishDate	2009
url	https://doaj.org/article/634f361556e242b28f07d9b20ba75109
work_keys_str_mv	AT kojisakamoto heterochronicshiftinhoxmediatedactivationofsonichedgehogleadstomorphologicalchangesduringfindevelopment AT kohonimaru heterochronicshiftinhoxmediatedactivationofsonichedgehogleadstomorphologicalchangesduringfindevelopment AT keijiromunakata heterochronicshiftinhoxmediatedactivationofsonichedgehogleadstomorphologicalchangesduringfindevelopment AT natsunosuda heterochronicshiftinhoxmediatedactivationofsonichedgehogleadstomorphologicalchangesduringfindevelopment AT mikatamura heterochronicshiftinhoxmediatedactivationofsonichedgehogleadstomorphologicalchangesduringfindevelopment AT harukiochi heterochronicshiftinhoxmediatedactivationofsonichedgehogleadstomorphologicalchangesduringfindevelopment AT mikikotanaka heterochronicshiftinhoxmediatedactivationofsonichedgehogleadstomorphologicalchangesduringfindevelopment
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Heterochronic shift in Hox-mediated activation of sonic hedgehog leads to morphological changes during fin development.

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