Endophytic bacteria in toxic South African plants: identification, phylogeny and possible involvement in gousiekte.

<h4>Background</h4>South African plant species of the genera Fadogia, Pavetta and Vangueria (all belonging to Rubiaceae) are known to cause gousiekte (literally 'quick disease'), a fatal cardiotoxicosis of ruminants characterised by acute heart failure four to eight weeks after...

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Autores principales:	Brecht Verstraete, Daan Van Elst, Hester Steyn, Braam Van Wyk, Benny Lemaire, Erik Smets, Steven Dessein
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/635245ae8b4e4bb29aeaec2766f07188
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spelling	oai:doaj.org-article:635245ae8b4e4bb29aeaec2766f071882021-11-18T06:55:13ZEndophytic bacteria in toxic South African plants: identification, phylogeny and possible involvement in gousiekte.1932-620310.1371/journal.pone.0019265https://doaj.org/article/635245ae8b4e4bb29aeaec2766f071882011-04-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21541284/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>South African plant species of the genera Fadogia, Pavetta and Vangueria (all belonging to Rubiaceae) are known to cause gousiekte (literally 'quick disease'), a fatal cardiotoxicosis of ruminants characterised by acute heart failure four to eight weeks after ingestion. Noteworthy is that all these plants harbour endophytes in their leaves: nodulating bacteria in specialized nodules in Pavetta and non-nodulating bacteria in the intercellular spaces between mesophyll cells in Fadogia and Vangueria.<h4>Principal findings</h4>Isolation and analyses of these endophytes reveal the presence of Burkholderia bacteria in all the plant species implicated in gousiekte. Although the nodulating and non-nodulating bacteria belong to the same genus, they are phylogenetically not closely related and even fall in different bacterial clades. Pavetta harborii and Pavetta schumanniana have their own specific endophyte--Candidatus Burkholderia harborii and Candidatus Burkholderia schumanniana--while the non-nodulating bacteria found in the other gousiekte-inducing plants show high similarity to Burkholderia caledonica. In this group, the bacteria are host specific at population level. Investigation of gousiekte-inducing plants from other African countries resulted in the discovery of the same endophytes. Several other plants of the genera Afrocanthium, Canthium, Keetia, Psydrax, Pygmaeothamnus and Pyrostria were tested and were found to lack bacterial endophytes.<h4>Conclusions</h4>The discovery and identification of Burkholderia bacteria in gousiekte-inducing plants open new perspectives and opportunities for research not only into the cause of this economically important disease, but also into the evolution and functional significance of bacterial endosymbiosis in Rubiaceae. Other South African Rubiaceae that grow in the same area as the gousiekte-inducing plants were found to lack bacterial endophytes which suggests a link between bacteria and gousiekte. The same bacteria are consistently found in gousiekte-inducing plants from different regions indicating that these plants will also be toxic to ruminants in other African countries.Brecht VerstraeteDaan Van ElstHester SteynBraam Van WykBenny LemaireErik SmetsSteven DesseinPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 4, p e19265 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Brecht Verstraete Daan Van Elst Hester Steyn Braam Van Wyk Benny Lemaire Erik Smets Steven Dessein Endophytic bacteria in toxic South African plants: identification, phylogeny and possible involvement in gousiekte.
description	<h4>Background</h4>South African plant species of the genera Fadogia, Pavetta and Vangueria (all belonging to Rubiaceae) are known to cause gousiekte (literally 'quick disease'), a fatal cardiotoxicosis of ruminants characterised by acute heart failure four to eight weeks after ingestion. Noteworthy is that all these plants harbour endophytes in their leaves: nodulating bacteria in specialized nodules in Pavetta and non-nodulating bacteria in the intercellular spaces between mesophyll cells in Fadogia and Vangueria.<h4>Principal findings</h4>Isolation and analyses of these endophytes reveal the presence of Burkholderia bacteria in all the plant species implicated in gousiekte. Although the nodulating and non-nodulating bacteria belong to the same genus, they are phylogenetically not closely related and even fall in different bacterial clades. Pavetta harborii and Pavetta schumanniana have their own specific endophyte--Candidatus Burkholderia harborii and Candidatus Burkholderia schumanniana--while the non-nodulating bacteria found in the other gousiekte-inducing plants show high similarity to Burkholderia caledonica. In this group, the bacteria are host specific at population level. Investigation of gousiekte-inducing plants from other African countries resulted in the discovery of the same endophytes. Several other plants of the genera Afrocanthium, Canthium, Keetia, Psydrax, Pygmaeothamnus and Pyrostria were tested and were found to lack bacterial endophytes.<h4>Conclusions</h4>The discovery and identification of Burkholderia bacteria in gousiekte-inducing plants open new perspectives and opportunities for research not only into the cause of this economically important disease, but also into the evolution and functional significance of bacterial endosymbiosis in Rubiaceae. Other South African Rubiaceae that grow in the same area as the gousiekte-inducing plants were found to lack bacterial endophytes which suggests a link between bacteria and gousiekte. The same bacteria are consistently found in gousiekte-inducing plants from different regions indicating that these plants will also be toxic to ruminants in other African countries.
format	article
author	Brecht Verstraete Daan Van Elst Hester Steyn Braam Van Wyk Benny Lemaire Erik Smets Steven Dessein
author_facet	Brecht Verstraete Daan Van Elst Hester Steyn Braam Van Wyk Benny Lemaire Erik Smets Steven Dessein
author_sort	Brecht Verstraete
title	Endophytic bacteria in toxic South African plants: identification, phylogeny and possible involvement in gousiekte.
title_short	Endophytic bacteria in toxic South African plants: identification, phylogeny and possible involvement in gousiekte.
title_full	Endophytic bacteria in toxic South African plants: identification, phylogeny and possible involvement in gousiekte.
title_fullStr	Endophytic bacteria in toxic South African plants: identification, phylogeny and possible involvement in gousiekte.
title_full_unstemmed	Endophytic bacteria in toxic South African plants: identification, phylogeny and possible involvement in gousiekte.
title_sort	endophytic bacteria in toxic south african plants: identification, phylogeny and possible involvement in gousiekte.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/635245ae8b4e4bb29aeaec2766f07188
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Endophytic bacteria in toxic South African plants: identification, phylogeny and possible involvement in gousiekte.

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