Structural and quantitative alterations of gut microbiota in experimental small bowel obstruction.

<h4>Objective</h4>To investigate structural and quantitative alterations of gut microbiota in an experimental model of small bowel obstruction.<h4>Method</h4>A rat model of small bowel obstruction was established by using a polyvinyl chloride ring surgically placed surroundin...

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Autores principales:	Jiali Mo, Lei Gao, Nan Zhang, Jiliang Xie, Donghua Li, Tao Shan, Liuyang Fan
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/6352e10239d345e89ee9b1d204f79b75
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spelling	oai:doaj.org-article:6352e10239d345e89ee9b1d204f79b752021-12-02T20:15:16ZStructural and quantitative alterations of gut microbiota in experimental small bowel obstruction.1932-620310.1371/journal.pone.0255651https://doaj.org/article/6352e10239d345e89ee9b1d204f79b752021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0255651https://doaj.org/toc/1932-6203<h4>Objective</h4>To investigate structural and quantitative alterations of gut microbiota in an experimental model of small bowel obstruction.<h4>Method</h4>A rat model of small bowel obstruction was established by using a polyvinyl chloride ring surgically placed surrounding the terminal ileum. The alterations of gut microbiota were studied after intestinal obstruction. Intraluminal fecal samples proximal to the obstruction were collected at different time points (24, 48 and 72 hours after obstruction) and analyzed by 16s rDNA high-throughput sequencing technology and quantitative PCR (qPCR) for target bacterial groups. Furthermore, intestinal claudin-1 mRNA expression was examined by real-time polymerase chain reaction analysis, and serum sIgA, IFABP and TFF3 levels were determined by enzyme-linked immunosorbent assay.<h4>Results</h4>Small bowel obstruction led to significant bacterial overgrowth and profound alterations in gut microbiota composition and diversity. At the phylum level, the 16S rDNA sequences showed a marked decrease in the relative abundance of Firmicutes and increased abundance of Proteobacteria, Verrucomicrobia and Bacteroidetes. The qPCR analysis showed the absolute quantity of total bacteria increased significantly within 24 hours but did not change distinctly from 24 to 72 hours. Further indicators of intestinal mucosa damage and were observed as claudin-1 gene expression, sIgA and TFF3 levels decreased and IFABP level increased with prolonged obstruction.<h4>Conclusion</h4>Small bowel obstruction can cause significant structural and quantitative alterations of gut microbiota and induce disruption of gut mucosa barrier.Jiali MoLei GaoNan ZhangJiliang XieDonghua LiTao ShanLiuyang FanPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 8, p e0255651 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jiali Mo Lei Gao Nan Zhang Jiliang Xie Donghua Li Tao Shan Liuyang Fan Structural and quantitative alterations of gut microbiota in experimental small bowel obstruction.
description	<h4>Objective</h4>To investigate structural and quantitative alterations of gut microbiota in an experimental model of small bowel obstruction.<h4>Method</h4>A rat model of small bowel obstruction was established by using a polyvinyl chloride ring surgically placed surrounding the terminal ileum. The alterations of gut microbiota were studied after intestinal obstruction. Intraluminal fecal samples proximal to the obstruction were collected at different time points (24, 48 and 72 hours after obstruction) and analyzed by 16s rDNA high-throughput sequencing technology and quantitative PCR (qPCR) for target bacterial groups. Furthermore, intestinal claudin-1 mRNA expression was examined by real-time polymerase chain reaction analysis, and serum sIgA, IFABP and TFF3 levels were determined by enzyme-linked immunosorbent assay.<h4>Results</h4>Small bowel obstruction led to significant bacterial overgrowth and profound alterations in gut microbiota composition and diversity. At the phylum level, the 16S rDNA sequences showed a marked decrease in the relative abundance of Firmicutes and increased abundance of Proteobacteria, Verrucomicrobia and Bacteroidetes. The qPCR analysis showed the absolute quantity of total bacteria increased significantly within 24 hours but did not change distinctly from 24 to 72 hours. Further indicators of intestinal mucosa damage and were observed as claudin-1 gene expression, sIgA and TFF3 levels decreased and IFABP level increased with prolonged obstruction.<h4>Conclusion</h4>Small bowel obstruction can cause significant structural and quantitative alterations of gut microbiota and induce disruption of gut mucosa barrier.
format	article
author	Jiali Mo Lei Gao Nan Zhang Jiliang Xie Donghua Li Tao Shan Liuyang Fan
author_facet	Jiali Mo Lei Gao Nan Zhang Jiliang Xie Donghua Li Tao Shan Liuyang Fan
author_sort	Jiali Mo
title	Structural and quantitative alterations of gut microbiota in experimental small bowel obstruction.
title_short	Structural and quantitative alterations of gut microbiota in experimental small bowel obstruction.
title_full	Structural and quantitative alterations of gut microbiota in experimental small bowel obstruction.
title_fullStr	Structural and quantitative alterations of gut microbiota in experimental small bowel obstruction.
title_full_unstemmed	Structural and quantitative alterations of gut microbiota in experimental small bowel obstruction.
title_sort	structural and quantitative alterations of gut microbiota in experimental small bowel obstruction.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/6352e10239d345e89ee9b1d204f79b75
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Structural and quantitative alterations of gut microbiota in experimental small bowel obstruction.

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