A cancer stem cell model as the point of origin of cancer-associated fibroblasts in tumor microenvironment

Abstract Cancer-associated fibroblasts (CAFs) are one of the most prominent cell types in the stromal compartment of the tumor microenvironment. CAFs support multiple aspects of cancer progression, including tumor initiation, invasion, and metastasis. The heterogeneous nature of the stromal microenv...

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Autores principales:	Neha Nair, Anna Sanchez Calle, Maram Hussein Zahra, Marta Prieto-Vila, Aung Ko Ko Oo, Laura Hurley, Arun Vaidyanath, Akimasa Seno, Junko Masuda, Yoshiaki Iwasaki, Hiromi Tanaka, Tomonari Kasai, Masaharu Seno
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/63542ceb2b9e4c0181ab0651968d562c
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spelling	oai:doaj.org-article:63542ceb2b9e4c0181ab0651968d562c2021-12-02T15:05:33ZA cancer stem cell model as the point of origin of cancer-associated fibroblasts in tumor microenvironment10.1038/s41598-017-07144-52045-2322https://doaj.org/article/63542ceb2b9e4c0181ab0651968d562c2017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-07144-5https://doaj.org/toc/2045-2322Abstract Cancer-associated fibroblasts (CAFs) are one of the most prominent cell types in the stromal compartment of the tumor microenvironment. CAFs support multiple aspects of cancer progression, including tumor initiation, invasion, and metastasis. The heterogeneous nature of the stromal microenvironment is attributed to the multiple sources from which the cells in this compartment originate. The present study provides the first evidence that cancer stem cells (CSCs) are one of the key sources of CAFs in the tumor niche. We generated CSC-like cells by treating mouse induced pluripotent stem cells with conditioned medium from breast cancer cell lines. The resulting cell population expressed both CSC and pluripotency markers, and the sphere-forming CSC-like cells formed subcutaneous tumors in nude mice. Intriguingly, these CSC-like cells always formed heterogeneous populations surrounded by myofibroblast-like cells. Based on this observation, we hypothesized that CSCs could be the source of the CAFs that support tumor maintenance and survival. To address this hypothesis, we induced the differentiation of spheres and purified the myofibroblast-like cells. The resulting cells exhibited a CAF-like phenotype, suggesting that they had differentiated into the subpopulations of cells that support CSC self-renewal. These findings provide novel insights into the dynamic interplay between various microenvironmental factors and CAFs in the CSC niche.Neha NairAnna Sanchez CalleMaram Hussein ZahraMarta Prieto-VilaAung Ko Ko OoLaura HurleyArun VaidyanathAkimasa SenoJunko MasudaYoshiaki IwasakiHiromi TanakaTomonari KasaiMasaharu SenoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-13 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Neha Nair Anna Sanchez Calle Maram Hussein Zahra Marta Prieto-Vila Aung Ko Ko Oo Laura Hurley Arun Vaidyanath Akimasa Seno Junko Masuda Yoshiaki Iwasaki Hiromi Tanaka Tomonari Kasai Masaharu Seno A cancer stem cell model as the point of origin of cancer-associated fibroblasts in tumor microenvironment
description	Abstract Cancer-associated fibroblasts (CAFs) are one of the most prominent cell types in the stromal compartment of the tumor microenvironment. CAFs support multiple aspects of cancer progression, including tumor initiation, invasion, and metastasis. The heterogeneous nature of the stromal microenvironment is attributed to the multiple sources from which the cells in this compartment originate. The present study provides the first evidence that cancer stem cells (CSCs) are one of the key sources of CAFs in the tumor niche. We generated CSC-like cells by treating mouse induced pluripotent stem cells with conditioned medium from breast cancer cell lines. The resulting cell population expressed both CSC and pluripotency markers, and the sphere-forming CSC-like cells formed subcutaneous tumors in nude mice. Intriguingly, these CSC-like cells always formed heterogeneous populations surrounded by myofibroblast-like cells. Based on this observation, we hypothesized that CSCs could be the source of the CAFs that support tumor maintenance and survival. To address this hypothesis, we induced the differentiation of spheres and purified the myofibroblast-like cells. The resulting cells exhibited a CAF-like phenotype, suggesting that they had differentiated into the subpopulations of cells that support CSC self-renewal. These findings provide novel insights into the dynamic interplay between various microenvironmental factors and CAFs in the CSC niche.
format	article
author	Neha Nair Anna Sanchez Calle Maram Hussein Zahra Marta Prieto-Vila Aung Ko Ko Oo Laura Hurley Arun Vaidyanath Akimasa Seno Junko Masuda Yoshiaki Iwasaki Hiromi Tanaka Tomonari Kasai Masaharu Seno
author_facet	Neha Nair Anna Sanchez Calle Maram Hussein Zahra Marta Prieto-Vila Aung Ko Ko Oo Laura Hurley Arun Vaidyanath Akimasa Seno Junko Masuda Yoshiaki Iwasaki Hiromi Tanaka Tomonari Kasai Masaharu Seno
author_sort	Neha Nair
title	A cancer stem cell model as the point of origin of cancer-associated fibroblasts in tumor microenvironment
title_short	A cancer stem cell model as the point of origin of cancer-associated fibroblasts in tumor microenvironment
title_full	A cancer stem cell model as the point of origin of cancer-associated fibroblasts in tumor microenvironment
title_fullStr	A cancer stem cell model as the point of origin of cancer-associated fibroblasts in tumor microenvironment
title_full_unstemmed	A cancer stem cell model as the point of origin of cancer-associated fibroblasts in tumor microenvironment
title_sort	cancer stem cell model as the point of origin of cancer-associated fibroblasts in tumor microenvironment
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/63542ceb2b9e4c0181ab0651968d562c
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A cancer stem cell model as the point of origin of cancer-associated fibroblasts in tumor microenvironment

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