CRISPR-dCas9 and sgRNA scaffolds enable dual-colour live imaging of satellite sequences and repeat-enriched individual loci

The ability to target Cas9 to specific genomic loci offers the potential for applications beyond genome editing. Here the authors use deactivated Cas9 and chimeric guide RNAs to visualise individual repetitive loci in living cells.

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Autores principales:	Yi Fu, Pedro P. Rocha, Vincent M. Luo, Ramya Raviram, Yan Deng, Esteban O. Mazzoni, Jane A. Skok
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2016
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/635bafc37e314ac384163cddf0d14810
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spelling	oai:doaj.org-article:635bafc37e314ac384163cddf0d148102021-12-02T14:39:29ZCRISPR-dCas9 and sgRNA scaffolds enable dual-colour live imaging of satellite sequences and repeat-enriched individual loci10.1038/ncomms117072041-1723https://doaj.org/article/635bafc37e314ac384163cddf0d148102016-05-01T00:00:00Zhttps://doi.org/10.1038/ncomms11707https://doaj.org/toc/2041-1723The ability to target Cas9 to specific genomic loci offers the potential for applications beyond genome editing. Here the authors use deactivated Cas9 and chimeric guide RNAs to visualise individual repetitive loci in living cells.Yi FuPedro P. RochaVincent M. LuoRamya RaviramYan DengEsteban O. MazzoniJane A. SkokNature PortfolioarticleScienceQENNature Communications, Vol 7, Iss 1, Pp 1-8 (2016)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Yi Fu Pedro P. Rocha Vincent M. Luo Ramya Raviram Yan Deng Esteban O. Mazzoni Jane A. Skok CRISPR-dCas9 and sgRNA scaffolds enable dual-colour live imaging of satellite sequences and repeat-enriched individual loci
description	The ability to target Cas9 to specific genomic loci offers the potential for applications beyond genome editing. Here the authors use deactivated Cas9 and chimeric guide RNAs to visualise individual repetitive loci in living cells.
format	article
author	Yi Fu Pedro P. Rocha Vincent M. Luo Ramya Raviram Yan Deng Esteban O. Mazzoni Jane A. Skok
author_facet	Yi Fu Pedro P. Rocha Vincent M. Luo Ramya Raviram Yan Deng Esteban O. Mazzoni Jane A. Skok
author_sort	Yi Fu
title	CRISPR-dCas9 and sgRNA scaffolds enable dual-colour live imaging of satellite sequences and repeat-enriched individual loci
title_short	CRISPR-dCas9 and sgRNA scaffolds enable dual-colour live imaging of satellite sequences and repeat-enriched individual loci
title_full	CRISPR-dCas9 and sgRNA scaffolds enable dual-colour live imaging of satellite sequences and repeat-enriched individual loci
title_fullStr	CRISPR-dCas9 and sgRNA scaffolds enable dual-colour live imaging of satellite sequences and repeat-enriched individual loci
title_full_unstemmed	CRISPR-dCas9 and sgRNA scaffolds enable dual-colour live imaging of satellite sequences and repeat-enriched individual loci
title_sort	crispr-dcas9 and sgrna scaffolds enable dual-colour live imaging of satellite sequences and repeat-enriched individual loci
publisher	Nature Portfolio
publishDate	2016
url	https://doaj.org/article/635bafc37e314ac384163cddf0d14810
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CRISPR-dCas9 and sgRNA scaffolds enable dual-colour live imaging of satellite sequences and repeat-enriched individual loci

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