Development of a Novel Zebrafish Model for Type 2 Diabetes Mellitus

Abstract Obesity is a major cause of type 2 diabetes mellitus (T2DM) in mammals. We have previously established a zebrafish model of diet-induced obesity (DIO zebrafish) by overfeeding Artemia. Here we created DIO zebrafish using a different method to induce T2DM. Zebrafish were overfed a commercial...

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Autores principales:	Liqing Zang, Yasuhito Shimada, Norihiro Nishimura
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/6363adec1a0e48979f37fee6163fc4fa
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spelling	oai:doaj.org-article:6363adec1a0e48979f37fee6163fc4fa2021-12-02T15:05:32ZDevelopment of a Novel Zebrafish Model for Type 2 Diabetes Mellitus10.1038/s41598-017-01432-w2045-2322https://doaj.org/article/6363adec1a0e48979f37fee6163fc4fa2017-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-01432-whttps://doaj.org/toc/2045-2322Abstract Obesity is a major cause of type 2 diabetes mellitus (T2DM) in mammals. We have previously established a zebrafish model of diet-induced obesity (DIO zebrafish) by overfeeding Artemia. Here we created DIO zebrafish using a different method to induce T2DM. Zebrafish were overfed a commercially available fish food using an automated feeding system. We monitored the fasting blood glucose levels in the normal-fed group (one feed/day) and overfed group (six feeds/day) over an 8-week period. The fasting blood glucose level was significantly increased in DIO zebrafish compared with that of normal-fed zebrafish. Intraperitoneal and oral glucose tolerance tests showed impaired glucose tolerance by overfeeding. Insulin production, which was determined indirectly by measuring the EGFP signal strength in overfed Tg(−1.0ins:EGFP) sc1 zebrafish, was increased in DIO zebrafish. The anti-diabetic drugs metformin and glimepiride ameliorated hyperglycaemia in the overfed group, suggesting that this zebrafish can be used as a model of human T2DM. Finally, we conducted RNA deep sequencing and found that the gene expression profiling of liver-pancreas revealed pathways common to human T2DM. In summary, we developed a zebrafish model of T2DM that shows promise as a platform for mechanistic and therapeutic studies of diet-induced glucose intolerance and insulin resistance.Liqing ZangYasuhito ShimadaNorihiro NishimuraNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Liqing Zang Yasuhito Shimada Norihiro Nishimura Development of a Novel Zebrafish Model for Type 2 Diabetes Mellitus
description	Abstract Obesity is a major cause of type 2 diabetes mellitus (T2DM) in mammals. We have previously established a zebrafish model of diet-induced obesity (DIO zebrafish) by overfeeding Artemia. Here we created DIO zebrafish using a different method to induce T2DM. Zebrafish were overfed a commercially available fish food using an automated feeding system. We monitored the fasting blood glucose levels in the normal-fed group (one feed/day) and overfed group (six feeds/day) over an 8-week period. The fasting blood glucose level was significantly increased in DIO zebrafish compared with that of normal-fed zebrafish. Intraperitoneal and oral glucose tolerance tests showed impaired glucose tolerance by overfeeding. Insulin production, which was determined indirectly by measuring the EGFP signal strength in overfed Tg(−1.0ins:EGFP) sc1 zebrafish, was increased in DIO zebrafish. The anti-diabetic drugs metformin and glimepiride ameliorated hyperglycaemia in the overfed group, suggesting that this zebrafish can be used as a model of human T2DM. Finally, we conducted RNA deep sequencing and found that the gene expression profiling of liver-pancreas revealed pathways common to human T2DM. In summary, we developed a zebrafish model of T2DM that shows promise as a platform for mechanistic and therapeutic studies of diet-induced glucose intolerance and insulin resistance.
format	article
author	Liqing Zang Yasuhito Shimada Norihiro Nishimura
author_facet	Liqing Zang Yasuhito Shimada Norihiro Nishimura
author_sort	Liqing Zang
title	Development of a Novel Zebrafish Model for Type 2 Diabetes Mellitus
title_short	Development of a Novel Zebrafish Model for Type 2 Diabetes Mellitus
title_full	Development of a Novel Zebrafish Model for Type 2 Diabetes Mellitus
title_fullStr	Development of a Novel Zebrafish Model for Type 2 Diabetes Mellitus
title_full_unstemmed	Development of a Novel Zebrafish Model for Type 2 Diabetes Mellitus
title_sort	development of a novel zebrafish model for type 2 diabetes mellitus
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/6363adec1a0e48979f37fee6163fc4fa
work_keys_str_mv	AT liqingzang developmentofanovelzebrafishmodelfortype2diabetesmellitus AT yasuhitoshimada developmentofanovelzebrafishmodelfortype2diabetesmellitus AT norihironishimura developmentofanovelzebrafishmodelfortype2diabetesmellitus
_version_	1718388767852593152

Development of a Novel Zebrafish Model for Type 2 Diabetes Mellitus

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