Preparation of an anti-4,4′-dinitrocarbanilide monoclonal antibody and its application in an immunochromatographic assay for anticoccidial drugs
This study outlines the successful development and testing of an immunochromatographic test strip for the detection of the anticoccidial drug, nicarbazin in milk. The preparation and characterization of an anti-4,4′-dinitrocarbanilide monoclonal antibody was based on hapten re-4,4′-dinitrocarbanilid...
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Taylor & Francis Group
2018
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oai:doaj.org-article:6369a04bf7eb48c3a4334f5ebc2c89332021-11-26T11:19:47ZPreparation of an anti-4,4′-dinitrocarbanilide monoclonal antibody and its application in an immunochromatographic assay for anticoccidial drugs0954-01051465-344310.1080/09540105.2018.1523372https://doaj.org/article/6369a04bf7eb48c3a4334f5ebc2c89332018-01-01T00:00:00Zhttp://dx.doi.org/10.1080/09540105.2018.1523372https://doaj.org/toc/0954-0105https://doaj.org/toc/1465-3443This study outlines the successful development and testing of an immunochromatographic test strip for the detection of the anticoccidial drug, nicarbazin in milk. The preparation and characterization of an anti-4,4′-dinitrocarbanilide monoclonal antibody was based on hapten re-4,4′-dinitrocarbanilide. The monoclonal antibody was highly specific for 4, 4′-dinitrocarbanilide, with a half-life inhibitory concentration (IC50) of 3.46 ng/mL. The detection limit was 0.86 ng/mL. The linear range was 1.71–7 ng/mL. The cut-off values of colloidal gold-based immunochromatographic test paper in phosphate buffered saline (PBS) and in milk were 10 ng/mL, 100 ng/mL, respectively. These results were in good agreement with ELISA analyses and suggest that immunochromatographic test strips are a fast and sensitive method for the semi-quantitative and qualitative analysis of nicarbazin in milk.Aihong WuXiaoling WuQiankun ZhengLiguang XuHua KuangTaylor & Francis Grouparticlenicarbazinantibodyimmunochromatography assaymilkanticoccidial drugsAgriculture (General)S1-972Immunologic diseases. AllergyRC581-607ENFood and Agricultural Immunology, Vol 29, Iss 1, Pp 1162-1172 (2018) |
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DOAJ |
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nicarbazin antibody immunochromatography assay milk anticoccidial drugs Agriculture (General) S1-972 Immunologic diseases. Allergy RC581-607 |
| spellingShingle |
nicarbazin antibody immunochromatography assay milk anticoccidial drugs Agriculture (General) S1-972 Immunologic diseases. Allergy RC581-607 Aihong Wu Xiaoling Wu Qiankun Zheng Liguang Xu Hua Kuang Preparation of an anti-4,4′-dinitrocarbanilide monoclonal antibody and its application in an immunochromatographic assay for anticoccidial drugs |
| description |
This study outlines the successful development and testing of an immunochromatographic test strip for the detection of the anticoccidial drug, nicarbazin in milk. The preparation and characterization of an anti-4,4′-dinitrocarbanilide monoclonal antibody was based on hapten re-4,4′-dinitrocarbanilide. The monoclonal antibody was highly specific for 4, 4′-dinitrocarbanilide, with a half-life inhibitory concentration (IC50) of 3.46 ng/mL. The detection limit was 0.86 ng/mL. The linear range was 1.71–7 ng/mL. The cut-off values of colloidal gold-based immunochromatographic test paper in phosphate buffered saline (PBS) and in milk were 10 ng/mL, 100 ng/mL, respectively. These results were in good agreement with ELISA analyses and suggest that immunochromatographic test strips are a fast and sensitive method for the semi-quantitative and qualitative analysis of nicarbazin in milk. |
| format |
article |
| author |
Aihong Wu Xiaoling Wu Qiankun Zheng Liguang Xu Hua Kuang |
| author_facet |
Aihong Wu Xiaoling Wu Qiankun Zheng Liguang Xu Hua Kuang |
| author_sort |
Aihong Wu |
| title |
Preparation of an anti-4,4′-dinitrocarbanilide monoclonal antibody and its application in an immunochromatographic assay for anticoccidial drugs |
| title_short |
Preparation of an anti-4,4′-dinitrocarbanilide monoclonal antibody and its application in an immunochromatographic assay for anticoccidial drugs |
| title_full |
Preparation of an anti-4,4′-dinitrocarbanilide monoclonal antibody and its application in an immunochromatographic assay for anticoccidial drugs |
| title_fullStr |
Preparation of an anti-4,4′-dinitrocarbanilide monoclonal antibody and its application in an immunochromatographic assay for anticoccidial drugs |
| title_full_unstemmed |
Preparation of an anti-4,4′-dinitrocarbanilide monoclonal antibody and its application in an immunochromatographic assay for anticoccidial drugs |
| title_sort |
preparation of an anti-4,4′-dinitrocarbanilide monoclonal antibody and its application in an immunochromatographic assay for anticoccidial drugs |
| publisher |
Taylor & Francis Group |
| publishDate |
2018 |
| url |
https://doaj.org/article/6369a04bf7eb48c3a4334f5ebc2c8933 |
| work_keys_str_mv |
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