Lentinan from shiitake selectively attenuates AIM2 and non-canonical inflammasome activation while inducing pro-inflammatory cytokine production

Abstract Lentinan extracted from shiitake (Lentinula edodes) is a β-glucan that has been reported as an intravenous anti-tumor polysaccharide via enhancement of the host immune system. In this study, we determined the effect of lentinan on inflammasome activation, a multi-protein platform, in myeloi...

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Autores principales:	Huijeong Ahn, Eunsaem Jeon, Jin-Chul Kim, Seung Goo Kang, Sung-il Yoon, Hyun-Jeong Ko, Pyeung-Hyeun Kim, Geun-Shik Lee
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/636dd45c6b704796b8c914b24c25d3aa
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spelling	oai:doaj.org-article:636dd45c6b704796b8c914b24c25d3aa2021-12-02T12:32:07ZLentinan from shiitake selectively attenuates AIM2 and non-canonical inflammasome activation while inducing pro-inflammatory cytokine production10.1038/s41598-017-01462-42045-2322https://doaj.org/article/636dd45c6b704796b8c914b24c25d3aa2017-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-01462-4https://doaj.org/toc/2045-2322Abstract Lentinan extracted from shiitake (Lentinula edodes) is a β-glucan that has been reported as an intravenous anti-tumor polysaccharide via enhancement of the host immune system. In this study, we determined the effect of lentinan on inflammasome activation, a multi-protein platform, in myeloid cells. Mouse bone marrow-derived macrophages were treated with lentinan with/without inflammasome triggers, and maturation of interleukin (IL)-1β, IL-18, or caspase-1 was measured as a readout of inflammasome activation. As a result, lentinan selectively inhibited absent in melanoma 2 (AIM2) inflammasome activation. In addition, lentinan up-regulated pro-inflammatory cytokines and induced expression of inflammasome-related genes through toll-like receptor 4 signaling. Furthermore, we assessed the effect of lentinan on mice treated with Listeria monocytogenes or lipopolysaccharide as an AIM2 or non-canonical inflammasome-mediated model. Lentinan attenuated IL-1β secretion resulting from Listeria-mediated AIM2 inflammasome activation and reduced endotoxin lethality via inhibition of non-canonical inflammasome activation. Thus, lentinan is suggested as an anti-AIM2 and anti-non-canonical inflammasome candidate despite its up-regulation of cytokine expression.Huijeong AhnEunsaem JeonJin-Chul KimSeung Goo KangSung-il YoonHyun-Jeong KoPyeung-Hyeun KimGeun-Shik LeeNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-12 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Huijeong Ahn Eunsaem Jeon Jin-Chul Kim Seung Goo Kang Sung-il Yoon Hyun-Jeong Ko Pyeung-Hyeun Kim Geun-Shik Lee Lentinan from shiitake selectively attenuates AIM2 and non-canonical inflammasome activation while inducing pro-inflammatory cytokine production
description	Abstract Lentinan extracted from shiitake (Lentinula edodes) is a β-glucan that has been reported as an intravenous anti-tumor polysaccharide via enhancement of the host immune system. In this study, we determined the effect of lentinan on inflammasome activation, a multi-protein platform, in myeloid cells. Mouse bone marrow-derived macrophages were treated with lentinan with/without inflammasome triggers, and maturation of interleukin (IL)-1β, IL-18, or caspase-1 was measured as a readout of inflammasome activation. As a result, lentinan selectively inhibited absent in melanoma 2 (AIM2) inflammasome activation. In addition, lentinan up-regulated pro-inflammatory cytokines and induced expression of inflammasome-related genes through toll-like receptor 4 signaling. Furthermore, we assessed the effect of lentinan on mice treated with Listeria monocytogenes or lipopolysaccharide as an AIM2 or non-canonical inflammasome-mediated model. Lentinan attenuated IL-1β secretion resulting from Listeria-mediated AIM2 inflammasome activation and reduced endotoxin lethality via inhibition of non-canonical inflammasome activation. Thus, lentinan is suggested as an anti-AIM2 and anti-non-canonical inflammasome candidate despite its up-regulation of cytokine expression.
format	article
author	Huijeong Ahn Eunsaem Jeon Jin-Chul Kim Seung Goo Kang Sung-il Yoon Hyun-Jeong Ko Pyeung-Hyeun Kim Geun-Shik Lee
author_facet	Huijeong Ahn Eunsaem Jeon Jin-Chul Kim Seung Goo Kang Sung-il Yoon Hyun-Jeong Ko Pyeung-Hyeun Kim Geun-Shik Lee
author_sort	Huijeong Ahn
title	Lentinan from shiitake selectively attenuates AIM2 and non-canonical inflammasome activation while inducing pro-inflammatory cytokine production
title_short	Lentinan from shiitake selectively attenuates AIM2 and non-canonical inflammasome activation while inducing pro-inflammatory cytokine production
title_full	Lentinan from shiitake selectively attenuates AIM2 and non-canonical inflammasome activation while inducing pro-inflammatory cytokine production
title_fullStr	Lentinan from shiitake selectively attenuates AIM2 and non-canonical inflammasome activation while inducing pro-inflammatory cytokine production
title_full_unstemmed	Lentinan from shiitake selectively attenuates AIM2 and non-canonical inflammasome activation while inducing pro-inflammatory cytokine production
title_sort	lentinan from shiitake selectively attenuates aim2 and non-canonical inflammasome activation while inducing pro-inflammatory cytokine production
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/636dd45c6b704796b8c914b24c25d3aa
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Lentinan from shiitake selectively attenuates AIM2 and non-canonical inflammasome activation while inducing pro-inflammatory cytokine production

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