Extraction of short chain chitooligosaccharides from fungal biomass and their use as promoters of arbuscular mycorrhizal symbiosis

Abstract Short chain chitooligosaccharides (COs) are chitin derivative molecules involved in plant-fungus signaling during arbuscular mycorrhizal (AM) interactions. In host plants, COs activate a symbiotic signalling pathway that regulates AM-related gene expression. Furthermore, exogenous CO applic...

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Autores principales:	Andrea Crosino, Elisa Moscato, Marco Blangetti, Gennaro Carotenuto, Federica Spina, Simone Bordignon, Virginie Puech-Pagès, Laura Anfossi, Veronica Volpe, Cristina Prandi, Roberto Gobetto, Giovanna Cristina Varese, Andrea Genre
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/63707d0e0aaf4fc990651ac837c8d6ae
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spelling	oai:doaj.org-article:63707d0e0aaf4fc990651ac837c8d6ae2021-12-02T12:11:52ZExtraction of short chain chitooligosaccharides from fungal biomass and their use as promoters of arbuscular mycorrhizal symbiosis10.1038/s41598-021-83299-62045-2322https://doaj.org/article/63707d0e0aaf4fc990651ac837c8d6ae2021-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-83299-6https://doaj.org/toc/2045-2322Abstract Short chain chitooligosaccharides (COs) are chitin derivative molecules involved in plant-fungus signaling during arbuscular mycorrhizal (AM) interactions. In host plants, COs activate a symbiotic signalling pathway that regulates AM-related gene expression. Furthermore, exogenous CO application was shown to promote AM establishment, with a major interest for agricultural applications of AM fungi as biofertilizers. Currently, the main source of commercial COs is from the shrimp processing industry, but purification costs and environmental concerns limit the convenience of this approach. In an attempt to find a low cost and low impact alternative, this work aimed to isolate, characterize and test the bioactivity of COs from selected strains of phylogenetically distant filamentous fungi: Pleurotus ostreatus, Cunninghamella bertholletiae and Trichoderma viride. Our optimized protocol successfully isolated short chain COs from lyophilized fungal biomass. Fungal COs were more acetylated and displayed a higher biological activity compared to shrimp-derived COs, a feature that—alongside low production costs—opens promising perspectives for the large scale use of COs in agriculture.Andrea CrosinoElisa MoscatoMarco BlangettiGennaro CarotenutoFederica SpinaSimone BordignonVirginie Puech-PagèsLaura AnfossiVeronica VolpeCristina PrandiRoberto GobettoGiovanna Cristina VareseAndrea GenreNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Andrea Crosino Elisa Moscato Marco Blangetti Gennaro Carotenuto Federica Spina Simone Bordignon Virginie Puech-Pagès Laura Anfossi Veronica Volpe Cristina Prandi Roberto Gobetto Giovanna Cristina Varese Andrea Genre Extraction of short chain chitooligosaccharides from fungal biomass and their use as promoters of arbuscular mycorrhizal symbiosis
description	Abstract Short chain chitooligosaccharides (COs) are chitin derivative molecules involved in plant-fungus signaling during arbuscular mycorrhizal (AM) interactions. In host plants, COs activate a symbiotic signalling pathway that regulates AM-related gene expression. Furthermore, exogenous CO application was shown to promote AM establishment, with a major interest for agricultural applications of AM fungi as biofertilizers. Currently, the main source of commercial COs is from the shrimp processing industry, but purification costs and environmental concerns limit the convenience of this approach. In an attempt to find a low cost and low impact alternative, this work aimed to isolate, characterize and test the bioactivity of COs from selected strains of phylogenetically distant filamentous fungi: Pleurotus ostreatus, Cunninghamella bertholletiae and Trichoderma viride. Our optimized protocol successfully isolated short chain COs from lyophilized fungal biomass. Fungal COs were more acetylated and displayed a higher biological activity compared to shrimp-derived COs, a feature that—alongside low production costs—opens promising perspectives for the large scale use of COs in agriculture.
format	article
author	Andrea Crosino Elisa Moscato Marco Blangetti Gennaro Carotenuto Federica Spina Simone Bordignon Virginie Puech-Pagès Laura Anfossi Veronica Volpe Cristina Prandi Roberto Gobetto Giovanna Cristina Varese Andrea Genre
author_facet	Andrea Crosino Elisa Moscato Marco Blangetti Gennaro Carotenuto Federica Spina Simone Bordignon Virginie Puech-Pagès Laura Anfossi Veronica Volpe Cristina Prandi Roberto Gobetto Giovanna Cristina Varese Andrea Genre
author_sort	Andrea Crosino
title	Extraction of short chain chitooligosaccharides from fungal biomass and their use as promoters of arbuscular mycorrhizal symbiosis
title_short	Extraction of short chain chitooligosaccharides from fungal biomass and their use as promoters of arbuscular mycorrhizal symbiosis
title_full	Extraction of short chain chitooligosaccharides from fungal biomass and their use as promoters of arbuscular mycorrhizal symbiosis
title_fullStr	Extraction of short chain chitooligosaccharides from fungal biomass and their use as promoters of arbuscular mycorrhizal symbiosis
title_full_unstemmed	Extraction of short chain chitooligosaccharides from fungal biomass and their use as promoters of arbuscular mycorrhizal symbiosis
title_sort	extraction of short chain chitooligosaccharides from fungal biomass and their use as promoters of arbuscular mycorrhizal symbiosis
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/63707d0e0aaf4fc990651ac837c8d6ae
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Extraction of short chain chitooligosaccharides from fungal biomass and their use as promoters of arbuscular mycorrhizal symbiosis

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