Detection of SARS-CoV-2 by real-time PCR under challenging pre-analytical conditions reveals independence of swab media and cooling chain

Abstract With global demand for SARS-CoV-2 testing ever rising, shortages in commercially available viral transport media pose a serious problem for laboratories and health care providers. For reliable diagnosis of SARS-CoV-2 and other respiratory viruses, executed by Real-time PCR, the quality of r...

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Autores principales:	Sabrina Summer, Ralf Schmidt, Anna Nele Herdina, Isabella Krickl, Julia Madner, Georg Greiner, Florian J. Mayer, Nicole Perkmann-Nagele, Robert Strassl
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/6370a6edb7c14d6bac684370f738323a
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spelling	oai:doaj.org-article:6370a6edb7c14d6bac684370f738323a2021-12-02T14:33:57ZDetection of SARS-CoV-2 by real-time PCR under challenging pre-analytical conditions reveals independence of swab media and cooling chain10.1038/s41598-021-93028-82045-2322https://doaj.org/article/6370a6edb7c14d6bac684370f738323a2021-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-93028-8https://doaj.org/toc/2045-2322Abstract With global demand for SARS-CoV-2 testing ever rising, shortages in commercially available viral transport media pose a serious problem for laboratories and health care providers. For reliable diagnosis of SARS-CoV-2 and other respiratory viruses, executed by Real-time PCR, the quality of respiratory specimens, predominantly determined by transport and storage conditions, is crucial. Therefore, our aim was to explore the reliability of minimal transport media, comprising saline or the CDC recommended Viral Transport Media (HBSS VTM), for the diagnosis of SARS-CoV-2 and other respiratory viruses (influenza A, respiratory syncytial virus, adenovirus, rhinovirus and human metapneumovirus) compared to commercial products, such as the Universal Transport Media (UTM). We question the assumptions, that the choice of medium and temperature for storage and transport affect the accuracy of viral detection by RT-PCR. Both alternatives to the commercial transport medium (UTM), HBSS VTM or saline, allow adequate detection of SARS-CoV-2 and other respiratory viruses, regardless of storage temperatures up to 28 °C and storage times up to 28 days. Our study revealed the high resilience of SARS-CoV-2 and other respiratory viruses, enabling proper detection in clinical specimens even after long-time storage at high temperatures, independent of the transport medium’s composition.Sabrina SummerRalf SchmidtAnna Nele HerdinaIsabella KricklJulia MadnerGeorg GreinerFlorian J. MayerNicole Perkmann-NageleRobert StrasslNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Sabrina Summer Ralf Schmidt Anna Nele Herdina Isabella Krickl Julia Madner Georg Greiner Florian J. Mayer Nicole Perkmann-Nagele Robert Strassl Detection of SARS-CoV-2 by real-time PCR under challenging pre-analytical conditions reveals independence of swab media and cooling chain
description	Abstract With global demand for SARS-CoV-2 testing ever rising, shortages in commercially available viral transport media pose a serious problem for laboratories and health care providers. For reliable diagnosis of SARS-CoV-2 and other respiratory viruses, executed by Real-time PCR, the quality of respiratory specimens, predominantly determined by transport and storage conditions, is crucial. Therefore, our aim was to explore the reliability of minimal transport media, comprising saline or the CDC recommended Viral Transport Media (HBSS VTM), for the diagnosis of SARS-CoV-2 and other respiratory viruses (influenza A, respiratory syncytial virus, adenovirus, rhinovirus and human metapneumovirus) compared to commercial products, such as the Universal Transport Media (UTM). We question the assumptions, that the choice of medium and temperature for storage and transport affect the accuracy of viral detection by RT-PCR. Both alternatives to the commercial transport medium (UTM), HBSS VTM or saline, allow adequate detection of SARS-CoV-2 and other respiratory viruses, regardless of storage temperatures up to 28 °C and storage times up to 28 days. Our study revealed the high resilience of SARS-CoV-2 and other respiratory viruses, enabling proper detection in clinical specimens even after long-time storage at high temperatures, independent of the transport medium’s composition.
format	article
author	Sabrina Summer Ralf Schmidt Anna Nele Herdina Isabella Krickl Julia Madner Georg Greiner Florian J. Mayer Nicole Perkmann-Nagele Robert Strassl
author_facet	Sabrina Summer Ralf Schmidt Anna Nele Herdina Isabella Krickl Julia Madner Georg Greiner Florian J. Mayer Nicole Perkmann-Nagele Robert Strassl
author_sort	Sabrina Summer
title	Detection of SARS-CoV-2 by real-time PCR under challenging pre-analytical conditions reveals independence of swab media and cooling chain
title_short	Detection of SARS-CoV-2 by real-time PCR under challenging pre-analytical conditions reveals independence of swab media and cooling chain
title_full	Detection of SARS-CoV-2 by real-time PCR under challenging pre-analytical conditions reveals independence of swab media and cooling chain
title_fullStr	Detection of SARS-CoV-2 by real-time PCR under challenging pre-analytical conditions reveals independence of swab media and cooling chain
title_full_unstemmed	Detection of SARS-CoV-2 by real-time PCR under challenging pre-analytical conditions reveals independence of swab media and cooling chain
title_sort	detection of sars-cov-2 by real-time pcr under challenging pre-analytical conditions reveals independence of swab media and cooling chain
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/6370a6edb7c14d6bac684370f738323a
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Detection of SARS-CoV-2 by real-time PCR under challenging pre-analytical conditions reveals independence of swab media and cooling chain

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