Metabolomic analysis of Trichophyton rubrum and Microsporum canis during keratin degradation

Abstract Keratin is important and needed for the growth of dermatophytes in the host tissue. In turn, the ability to invade keratinised tissues is defined as a pivotal virulence attribute of this group of medically important fungi. The host–dermatophyte interaction is accompanied by an adaptation of...

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Autores principales:	Anita Ciesielska, Anna Kawa, Katarzyna Kanarek, Adrian Soboń, Rafał Szewczyk
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/6371ab0b28dc4c8cbe05f95dcba4c63c
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spelling	oai:doaj.org-article:6371ab0b28dc4c8cbe05f95dcba4c63c2021-12-02T12:11:12ZMetabolomic analysis of Trichophyton rubrum and Microsporum canis during keratin degradation10.1038/s41598-021-83632-z2045-2322https://doaj.org/article/6371ab0b28dc4c8cbe05f95dcba4c63c2021-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-83632-zhttps://doaj.org/toc/2045-2322Abstract Keratin is important and needed for the growth of dermatophytes in the host tissue. In turn, the ability to invade keratinised tissues is defined as a pivotal virulence attribute of this group of medically important fungi. The host–dermatophyte interaction is accompanied by an adaptation of fungal metabolism that allows them to adhere to the host tissue as well as utilize the available nutrients necessary for their survival and growth. Dermatophyte infections pose a significant epidemiological and clinical problem. Trichophyton rubrum is the most common anthropophilic dermatophyte worldwide and its typical infection areas include skin of hands or feet and nail plate. In turn, Microsporum canis is a zoophilic pathogen, and mostly well known for ringworm in pets, it is also known to infect humans. The aim of the study was to compare the intracellular metabolite content in the T. rubrum and M. canis during keratin degradation using liquid chromatography system coupled with tandem mass spectrometer (LC-MS/MS). The metabolite “fingerprints” revealed compounds associated with amino acids metabolism, carbohydrate metabolism related to the glycolysis and the tricarboxylic acid cycle (TCA), as well as nucleotide and energy metabolism. The metabolites such as kynurenic acid, l-alanine and cysteine in case of T. rubrum as well as cysteine and riboflavin in case of M. canis were detected only during keratin degradation what may suggest that these compounds may play a key role in the interactions of T. rubrum and M. canis with the host tissue. The metabolomic results were completed by qPCR gene expression assay. Our findings suggest that metabolomic analysis of T. rubrum and M. canis growing in culture media that mimic the dermatophyte infection could allow the understanding of processes involved in the pathogenesis of dermatophytes.Anita CiesielskaAnna KawaKatarzyna KanarekAdrian SobońRafał SzewczykNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Anita Ciesielska Anna Kawa Katarzyna Kanarek Adrian Soboń Rafał Szewczyk Metabolomic analysis of Trichophyton rubrum and Microsporum canis during keratin degradation
description	Abstract Keratin is important and needed for the growth of dermatophytes in the host tissue. In turn, the ability to invade keratinised tissues is defined as a pivotal virulence attribute of this group of medically important fungi. The host–dermatophyte interaction is accompanied by an adaptation of fungal metabolism that allows them to adhere to the host tissue as well as utilize the available nutrients necessary for their survival and growth. Dermatophyte infections pose a significant epidemiological and clinical problem. Trichophyton rubrum is the most common anthropophilic dermatophyte worldwide and its typical infection areas include skin of hands or feet and nail plate. In turn, Microsporum canis is a zoophilic pathogen, and mostly well known for ringworm in pets, it is also known to infect humans. The aim of the study was to compare the intracellular metabolite content in the T. rubrum and M. canis during keratin degradation using liquid chromatography system coupled with tandem mass spectrometer (LC-MS/MS). The metabolite “fingerprints” revealed compounds associated with amino acids metabolism, carbohydrate metabolism related to the glycolysis and the tricarboxylic acid cycle (TCA), as well as nucleotide and energy metabolism. The metabolites such as kynurenic acid, l-alanine and cysteine in case of T. rubrum as well as cysteine and riboflavin in case of M. canis were detected only during keratin degradation what may suggest that these compounds may play a key role in the interactions of T. rubrum and M. canis with the host tissue. The metabolomic results were completed by qPCR gene expression assay. Our findings suggest that metabolomic analysis of T. rubrum and M. canis growing in culture media that mimic the dermatophyte infection could allow the understanding of processes involved in the pathogenesis of dermatophytes.
format	article
author	Anita Ciesielska Anna Kawa Katarzyna Kanarek Adrian Soboń Rafał Szewczyk
author_facet	Anita Ciesielska Anna Kawa Katarzyna Kanarek Adrian Soboń Rafał Szewczyk
author_sort	Anita Ciesielska
title	Metabolomic analysis of Trichophyton rubrum and Microsporum canis during keratin degradation
title_short	Metabolomic analysis of Trichophyton rubrum and Microsporum canis during keratin degradation
title_full	Metabolomic analysis of Trichophyton rubrum and Microsporum canis during keratin degradation
title_fullStr	Metabolomic analysis of Trichophyton rubrum and Microsporum canis during keratin degradation
title_full_unstemmed	Metabolomic analysis of Trichophyton rubrum and Microsporum canis during keratin degradation
title_sort	metabolomic analysis of trichophyton rubrum and microsporum canis during keratin degradation
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/6371ab0b28dc4c8cbe05f95dcba4c63c
work_keys_str_mv	AT anitaciesielska metabolomicanalysisoftrichophytonrubrumandmicrosporumcanisduringkeratindegradation AT annakawa metabolomicanalysisoftrichophytonrubrumandmicrosporumcanisduringkeratindegradation AT katarzynakanarek metabolomicanalysisoftrichophytonrubrumandmicrosporumcanisduringkeratindegradation AT adriansobon metabolomicanalysisoftrichophytonrubrumandmicrosporumcanisduringkeratindegradation AT rafałszewczyk metabolomicanalysisoftrichophytonrubrumandmicrosporumcanisduringkeratindegradation
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Metabolomic analysis of Trichophyton rubrum and Microsporum canis during keratin degradation

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